Fingerprints from fingerprints

Balogh, Márta; Bürger, Joachim; Bender, Klaus; Schneider, P.M.; Alt, Kurt W.

doi:10.1016/s0531-5131(02)00230-3

Cited by 34 publications

(22 citation statements)

References 6 publications

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“…Secondary stain transfer could be demonstrated, but only very weak patterns were detected after 34 PCR cycles [6]. Additionally, it has to be kept in mind that secondary transfer can be obtained if forensically relevant crime scene items were sampled without changing gloves [5].…”

Section: Discussionmentioning

confidence: 99%

“…The increasing sensitivity of short tandem repeat (STR)-based stain analysis during the last years has enabled typing of very weak biological stains [1][2][3][4][5][6][7]. However, this improvement may jeopardise the reliability of DNA typing as epithelial cells or, more likely, blood or saliva could have been secondarily transferred from stains to skin areas of persons and from there to other items without notice of the recipient [3].…”

Section: Introductionmentioning

confidence: 99%

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Transfer of biological stains from different surfaces

Wiegand

Heimbold²,

Klein³

et al. 2010

Int J Legal Med

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Highly sensitive short tandem repeat-based stain analysis of weak biological stains has been improved during the last years. The risk of transfer of cellular material from handled items contaminated with biological stains such as blood and saliva is of forensic relevance. Although the policemen working with crime scene items are very careful, there exists a potential risk of transfer contamination. To obtain estimates for the risk of stain transfer by handling, we have carried out an experimental study on 288 dried blood and saliva stains in two laboratories. DNA quantification showed only small amounts of DNA that could be transferred, especially from stains on paper and cotton. The saliva and especially the blood stains from plastic surface resulted in higher amounts of transferred DNA, depending on the relation of blood volume to included area: Of 192 direct transfers, 17% gave extracts above 10 pg DNA/μl, and only 3% of 96 secondary transfers resulted in amounts above 10 pg DNA/μl.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transfer of biological stains from different surfaces

Wiegand

Heimbold²,

Klein³

et al. 2010

Int J Legal Med

View full text Add to dashboard Cite

show abstract

“…For comparison purposes, further extraction kits were carried out such as (ii) ChargeSwitch ® Forensic DNA Purifaction kit (Invitrogen) according to the manufacturer's instructions, (iii) Invisorb ® Spin Forensic kit (Invitek) as described in ref. [28] and a simple (iv) GEN-IAL extraction. At the end, DNA was precipitated and the pellet was dissolved in 30 mL of sterilized water.…”

Section: Methodsmentioning

confidence: 99%

Improved STR typing of telogen hair root and hair shaft DNA

et al. 2007

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Today the STR typing of telogen hair and hair shafts is regarded as a challenge. The small DNA quantity in the hair is highly degraded. Another problem are PCR inhibitors in the hair. In particular hair pigments, the melanins, are known to inhibit PCR. Hairs are exposed to sunlight and partly to chemical oxidation processes, which make them even more difficult to analyze. To increase the chances of a correct typing of hair, the small amount of DNA must be successfully isolated and the inhibitors have to be removed or neutralized. Furthermore, miniSTR typing improves the analysis of stains with degraded DNA like it is the case with hair. We introduce a nonorganic extraction method and in addition a miniSTR concept which is promising in typing stains with little and degraded DNA, especially hairs. The miniSTR concept including five database STRs (SE33, VWA, TH01, FGA, D3S1358) and the gender typing system Amelogenin was optimized for the amplification of hair DNA. Compared to commercial STR kits, this approach resulted in considerably higher success rates.

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“…This is especially true for minimal stains such as epidermal abrasions or handprints on different surfaces. Nevertheless, there are samples, especially epidermal abrasions, which sometimes are easy to analyse and sometimes still show poor results in DNA analysis [1,6,7]. Here, it might be difficult to explain to the police or at court why there are sometimes significant differences regarding genetic typing results from apparently similar traces.…”

Section: Introductionmentioning

confidence: 99%

Good shedder or bad shedder—the influence of skin diseases on forensic DNA analysis from epithelial abrasions

Kamphausen¹,

Schadendorf

Wurmb‐Schwark

et al. 2011

Int J Legal Med

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The successful analysis of weak biological stains by means of highly sensitive short tandem repeat (STR) amplification has been increased significantly over the recent years. Nevertheless, the percentage of reliably analysable samples varies considerably between different crime scene investigations even if the nature of the stains appears to be the same. It has been proposed that the amount and quality of DNA left at a crime scene may be due to individual skin conditions (among other factors). Therefore, we investigated DNA from handprints from 30 patients acutely suffering from skin diseases like atopic dermatitis, psoriasis or skin ulcer before and after therapy by STR amplification using the new and highly sensitive Powerplex® ESX17 kit in comparison to 22 healthy controls. Handprints from atopic dermatitis patients showed a correct and reliable DNA profile in 90% and 40% of patients before and after therapy, respectively. Regarding psoriasis patients, we detected full DNA profiles in only 64% and 55% of handprints before and after therapy. In contrast, in ulcus patients and controls, full DNA profiles were obtained in much lower numbers. We conclude that active skin diseases like atopic dermatitis or psoriasis have a considerable impact on the amplificable DNA left by skin contact with surfaces. Since up to 7% of adults in European countries suffer from one of these diseases, this could explain at least partially the varying quality of DNA from weak stains.

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Fingerprints from fingerprints

Cited by 34 publications

References 6 publications

Transfer of biological stains from different surfaces

Transfer of biological stains from different surfaces

Improved STR typing of telogen hair root and hair shaft DNA

Good shedder or bad shedder—the influence of skin diseases on forensic DNA analysis from epithelial abrasions

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