Fingerprints and DNA: STR Typing of DNA Extracted from Adhesive Tape after Processing for Fingerprints

Zamir, Ashira; Springer, Eliot; Glattstein, Baruch

doi:10.1520/jfs14749j

Cited by 54 publications

(26 citation statements)

References 2 publications

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“…No interference, but may depend on thickness of deposition and DNA extraction method Stein et al 1996;Zamir et al 2000b;von Wurmb et al 2001;Grubwieser et al 2003;Bille et al 2009 Chemical methods…”

Section: Evidence Collection and Preservation At The Crime Scene And mentioning

confidence: 99%

Forensic DNA Analysis

Prinz¹,

Lessig²

2014

Handbook of Forensic Medicine

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Section: Evidence Collection and Preservation At The Crime Scene And mentioning

confidence: 99%

Forensic DNA Analysis

Prinz¹,

Lessig²

2014

Handbook of Forensic Medicine

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“…It has previously been reported that fingerprinting techniques (including those using powder) do not hinder the obtaining of DNA profiles from treated samples (6,(11)(12)(13)(14)(15). If, however, fingerprinting powder were to remain in the DNA sample after extraction (as has on occasion been noted when performing Chelex extractions followed by using Centricons R and/or Microcons R to concentrate the sample) and inadvertently added to the PCR mix, would it inhibit the amplification?…”

Section: Experiments 3: Inhibitionmentioning

confidence: 99%

Beware of the Possibility of Fingerprinting Techniques Transferring DNA

Oorschot

Treadwell

Beaurepaire

et al. 2005

Journal of Forensic Sciences

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Fingerprinting brushes have the potential to collect and transfer DNA during powdering. Squirrel-hair fingerprint brushes exposed to specific sets of saliva stains and brushes used in routine casework were tested for their ability to collect and transfer DNA containing material using standard DNA extraction procedures and AmpFlSTR R Profiler Plus TM amplification and typing procedures. The tests found that the risk of transferring DNA during powdering and having a detrimental impact on the analysis increases if the examiner powders over either biological stains (such as blood or saliva) or very fresh prints and uses more sensitive PCR amplification and typing procedures. We advocate caution when powdering prints from which DNA may also be collected and provide options for consideration to limit the risk of transferred DNA contamination while fingerprinting.

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“…The standard procedure for fiber investigations was, and still is, to tape corpses, items of clothing or any other items of evidential value with a special kind of adhesive tape and to microscopically and painstakingly search these tapings step-by-step noting the position of any fibers suspected of having been transferred from one item to the other [1]. Fiber investigations nowadays play a subordinate role to DNA in forensic investigations, although they have not been completely discarded and can sometimes be of vital importance.…”

Section: Introductionmentioning

confidence: 99%

Hot flakes in cold cases

et al. 2011

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In the past, it was almost impossible for forensic scientists to separate DNA from an undefined number of different individuals in mixed stains where, for example, two or more suspects had handled the same weapon. Such samples often contain complex mixtures with the consequence of ambiguous or inconclusive mixed DNA profiles. Using the method described of comprehensive and/or targeted screening of shed cells adhering to tapings of garments or objects enables such stains to be individualized. To evaluate the method, 500 microscopically selected single skin flakes were analyzed using two different commercial STR kits to compare the success rates for each PCR typing system. The method has been validated for use in routine casework and has been shown to be rapid, sensitive, and reproducible. It can be predicted that many cases in the archives with body tapings, which have not yet been examined will benefit from this new or perhaps more appropriate, reanimated, technical development, and of particular importance are serious crimes, the so-called cold cases. The remarkable forensic value of this simple but time-consuming technique is exemplified by 2 out of approximately 100 cases already successfully solved using this approach.

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Fingerprints and DNA: STR Typing of DNA Extracted from Adhesive Tape after Processing for Fingerprints

Cited by 54 publications

References 2 publications

Forensic DNA Analysis

Forensic DNA Analysis

Beware of the Possibility of Fingerprinting Techniques Transferring DNA

Hot flakes in cold cases

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