C, O and Sr isotope geochemistry of high-grade marbles from the Lützow-Holm Complex, East Antarctica, has given clues on the depositional ages and post-depositional alterations. Dolomitic and calcitic marbles occur as thin layers with varying thickness (up to 100 m) in several outcrops in eastern Dronning Maud Land, most of which underwent postdepositional geochemical alterations. In particular, the Sr and O isotope alterations are extensive, with 87 Sr/ 86 Sr (550 Ma) ratios as high as 0.758 and d 18 O values as low as 25‰. These data suggest that multiple stages of fluid-rock interaction processes during diagenesis, prograde to peak and retrograde metamorphic events have altered the depositional isotopic signatures. However, some of the marble layers, exceptionally, preserve pre-metamorphic geochemical characteristics, such as low Sr isotope ratios, high d 18 O and d 13 C values, and well-equilibrated unaltered trace and rare earth element patterns. Lowest 87 Sr/ 86 Sr isotopic ratios of 0.7066 and 0.7053 with high d 13 C and d 18 O values suggest an apparent age of deposition around 730-830 Ma, although total geochemical resetting of carbonates by seawater of this age cannot be ruled out. The apparent depositional ages are consistent with carbonate deposition in the 'Mozambique Ocean' that separated East and West Gondwana.