Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis

Navalta, James W.; McFarlin, Brian K.; Simpson, Richard J.; Fedor, Elizabeth A.; Kell, Holly B.; Lyons, Scott; Arnett, Scott W.; Schafer, Mark A.

doi:10.3791/2595

Cited by 10 publications

(11 citation statements)

References 8 publications

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“…The lymphocyte subsets were CD4+, CD8+, and CD19+ with cell surface markers being annexin V and CX3CR1 (BioLegend, San Diego, CA, USA). The blood analysis protocol for the determination of lymphocyte subsets has been described elsewhere [ 17 ]. Whole blood (20 µL) was added to an appropriate antibody panel (250 µL) and incubated in a dark room for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Three Consecutive Days of Interval Runs to Exhaustion Affects Lymphocyte Subset Apoptosis and Migration

Navalta

Tibana

Fedor

et al. 2014

BioMed Research International

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This investigation assessed the lymphocyte subset response to three days of intermittent run exercise to exhaustion. Twelve healthy college-aged males (n = 8) and females (n = 4) (age = 26 ± 4 years; height = 170.2 ± 10 cm; body mass = 75 ± 18 kg) completed an exertion test (maximal running speed and VO2max) and later performed three consecutive days of an intermittent run protocol to exhaustion (30 sec at maximal running speed and 30 sec at half of the maximal running speed). Blood was collected before exercise (PRE) and immediately following the treadmill bout (POST) each day. When the absolute change from baseline was evaluated (i. e., Δ baseline), a significant change in CD4+ and CD8+ for CX3CR1 cells was observed by completion of the third day. Significant changes in both apoptosis and migration were observed following two consecutive days in CD19+ lymphocytes, and the influence of apoptosis persisted following the third day. Given these lymphocyte responses, it is recommended that a rest day be incorporated following two consecutive days of a high-intensity intermittent run program to minimize immune cell modulations and reduce potential susceptibility.

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Section: Methodsmentioning

confidence: 99%

Three Consecutive Days of Interval Runs to Exhaustion Affects Lymphocyte Subset Apoptosis and Migration

Navalta

Tibana

Fedor

et al. 2014

BioMed Research International

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“…1 . Before each RE session, subjects rested for 10 min, and a blood sample was obtained (PRE) by fi nger-prick procedures [ 6 ] . Subjects also had their blood collected immediately post-exercise (POST), 2 h and 24 h after exercise.…”

Section: Methodsmentioning

confidence: 99%

“…Finger-prick blood samples were obtained before familiarization, PRE, POST, 2 h and 24 h after the experimental sessions. The protocol of blood analysis for lymphocyte subsets has been described previously [ 6 ] . Whole blood (20 μL) was added to an appropriate antibody panel (250 μL) and incubated in a dark room for 30 min.…”

Section: Blood Analysismentioning

confidence: 99%

The Acute Response of Apoptosis and Migration to Resistance Exercise Is Protocol-Dependent

et al. 2014

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The aim of the present study was to compare the acute effects of resistance exercise (RE) designed for hypertrophy or local muscle endurance (LME) on CD4+ and CD8+ T cell apoptosis and migration. 14 untrained subjects (age 20.5±0.8 years, body mass 70.0±12.8 kg, body mass index 24.0±3.2 kg/m(2)), women (N=11) and men (N=3) completed 2 RE sessions (3 sets of 9 exercises) designed for hypertrophy at 10 repetitions maximum (RM) and LME at 60% of 10RM with 1-min rest-intervals between sets and exercises. The investigated lymphocytes were: CD4+, CD4+/CD69RA+, CD8+ and CD8+/CD69RA+ with cell surface markers annexin V and CX3CR1 analyzed by flow cytometry. Percentage of CD4+ positive for annexin V+ were higher immediately following and 24 h after the hypertrophy protocol as compared with LME, while CD4+ positive for CX3CR1 were higher immediately after and lower at the 24 h time point after LME as compared with the hypertrophy session. CD8+ lymphocytes responded similarly to the hypertrophy and LME protocols with elevations in both cellular migration and cell death immediately following and 24 h after the bouts (p≤0.05). Considering that the acute response of CD4+ lymphocytes to RE is protocol-dependent, a gradual adaptation to a hypertrophy program could minimize the effect on CD4+ lymphocytes and reduce the potential susceptibility to antigens during this timeframe. This would also be interesting for a RE program designed for LME based on the observed CD8+ lymphocyte response.

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“…Blood was sampled at rest, following the 76 % and 87 % runs, immediately after exercise (i. e., 100 % VO 2max ), and 1-h post exercise. Whole blood was obtained via fi nger-stick into heparanized capillary tubes (Globe Scientifi c Inc., Paramus, NJ) and processed as described previously [ 13 ] . Briefl y, whole blood was added to panels containing staining buff er and tittered concentrations of antibodies including CD3, CD4, CD8, CD19, annexin V and CX 3 CR1 (BioLegend, San Diego, CA) and incubated for 30 min in the dark at room temperature.…”

Section: Blood Processingmentioning

confidence: 99%

Exercise Intensity and Lymphocyte Subset Apoptosis

et al. 2012

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This investigation assessed the lymphocyte subset response to increasing intensity. Participants completed an exertion test (VO(2max)), and later performed a 10-min run at 76% VO(2max), 5-min at 87%, and run to exhaustion at 100% intensity. Blood was sampled at rest, following each intensity, and 1-h post. Cell concentration, apoptosis (annexin V) and migration (CX₃CR1) were evaluated in CD4+, CD8+, and CD19+ subsets. Relative data were analyzed using 1-way ANOVA with significance at P≤0.05. Absolute changes from rest (Δ baseline) were calculated for exercise conditions. CX₃CR1 displayed relative changes 1-h post, (CD8+ Pre=58%, Post=68%, 1 h-Post=37%, P=0.04) (CD19+ Pre=1.9%, Post=3.2%, 1 h-Post=5.2%, P=0.02). No relative changes were noted for subsets and annexin V. Absolute changes revealed that CD4+/annexin V+ and CD8+/annexin V+ significantly increased at 76%,(P<0.01). Significant absolute increases were observed in CD4+/CX₃CR1 at 87% VO2max, and at 87% and 100% VO2max in CD8+/CX₃CR1 (P<0.01). Subsets respond differently with intensity with respect to cell count, and markers of apoptosis and cell migration. CD4+ and CD8+ appear to be prone to apoptosis with moderate exercise, but significant increases in migration at higher intensities suggests movement of these cells from the vasculature in postexercise measurements.

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Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis

Cited by 10 publications

References 8 publications

Three Consecutive Days of Interval Runs to Exhaustion Affects Lymphocyte Subset Apoptosis and Migration

Three Consecutive Days of Interval Runs to Exhaustion Affects Lymphocyte Subset Apoptosis and Migration

The Acute Response of Apoptosis and Migration to Resistance Exercise Is Protocol-Dependent

Exercise Intensity and Lymphocyte Subset Apoptosis

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