Finger Printing and Quantitative Analysis of <i>Cuscuta chinensis</i> Flavonoid Contents from Different Hosts by RP-HPLC

Abstract

Background: Cuscuta spp. known as dodders, have been used as traditional medicines in eastern and southern Asian countries for many disorders such as gastrointestinal, respiratory, endocrine, skin and neurological diseases (Drug of mania). Flavonoids are the main biologically active constituents in Cuscuta genus especially in C. chinensis. Our aim in this study was to identify and discriminate between C. chinensis samples which were collected from different hosts, by using the pattern recognition aided fingerprint analysis and comparison of the content of four major flavonoids (hyperoside, rutin, isorhamnetin, kaempferol). Material and methods: Samples were collected from nine different plants in the same time and place, dried, grinded and extracted with methanol (80%) by repeated maceration. Extractions were evaluated by high performance liquid chromatography analysis and fingerprinting. Results: Beside chromatographic fingerprint using similarity index, we compared the content of 4 major flavonoids (hyperoside, rutin, isorhamnetin, kaempferol) of C. chinensis samples on different hosts. Coclusion: The results showed that there were significant differences between the content of four major flavonoids of nine C. chinensis samples, but chromatographic fingerprint by similarity index of more than 0.88, showed that the sample consistency was similar. So, it was suggested that combination of chromatographic fingerprint and the content determination of major flavonoids could be used to evaluate the quality control of C. chinensis from different hosts.

Efficacy of Cuscuta campestris Yunck. (Solanales: Convolvulaceae) and Lupinus albus L. (Fabales: Fabaceae) crude extracts against nymphs and adults of Orosanga japonica (Melichar, 1898) (Hemiptera: Ricaniidae)

“…Depending on the results of the preliminary laboratory studies, seed (L. albus) and vegetative growing stage (C. campestris) of the plants to be studied were determined. This choice is supported by the literature (Shekarchi et al, 2014;Hassan et al, 2019). The specimen identification was performed by Prof. Vagif Atamov from the Faculty of Science and Arts, Recep Tayyip Erdogan University in Rize, Turkey.…”

“…have shown that phenolic and alkaloid contents of this plants are high. In addition, it has been observed that their potential to be natural alternative insecticides against many pests has been investigated (Thackray et al, 2000;Torres et al, 2009;Shekarchi et al, 2014;Selvi et al, 2017;Karamac et al, 2018;Hassan et al, 2019). However, no study has found effectiveness of the same plants against O. japonica.…”

Integrated Metabolites and Transcriptomics at Different Growth Stages Reveal Polysaccharide and Flavonoid Biosynthesis in Cynomorium songaricum

“…Although the optimum harvest time of the C. songaricum stem at EFS has been demonstrated in previous studies [12,13], the mechanism of polysaccharide and flavonoid biosynthesis during growth stages is still limited. In this study, the maximum values of stem fresh weight, soluble sugar and total flavonoids contents, and antioxidant capacity in C. songaricum were observed at EFS compared with GS, VGS, and FS; a total of 367 DEGs co-expressed at the GS, VGS, and EFS with 109 genes directly involved in polysaccharide and flavonoid biosynthesis as well as growth and development.…”

“…The types and contents of liposoluble components (e.g., β-sitosterol, hexadecanoic acid, and (Z)-9-octadecenoic acid) in C. songaricum were significantly different among different hosts [11]. For other parasitic plants, there were significant differences in contents of flavonoids (e.g., rutin, isorhamnetin, and kaempferol) in Cuscuta chinensis from different hosts [12]. Generally, the optimum harvest time of the C. songaricum stem is between the unearthed prophase (namely vegetative growth stage, VGS) and unearthed stage (namely early flowering stage, EFS), with greater contents of polysaccharides and protocatechuic acid compared with the flowering stage (FS) and fruiting stage [13,14].…”

Phytoconstituents From the Roots of Cuscuta Chinensis

“…Phytochemicals reported from the plant include 4-Hydroxy benzoic acid, paracoumaric acid, coumarin, vanillic acid, α-resorcylic acid, rutin, naringenin-7-rhamnoglucoside, marsileagenin A, 3-hydroxy triacontane-11-one, protocatechuic acid, caffeic acid, and ferulic acid. (Shekarchi et al, 2014) Keeping in view of its pharmacological properties, the authors have attempted to re-investigate the whole plant for its phytoconstituents and reported flavones salvigenin, chrisimaritin for the first time apart from the earlier reported quercetin and triterpenoid betulinic acid.…”