Fine-tuning of LA-ICP-QMS conditions for elemental mapping

Abstract

For high-speed and high-resolution elemental LA-ICP-QMS mapping it is important to select the optimal operational conditions to avoid aliasing, minimize blur and maximize the signal-to-noise ratio. An earlier paper already...

An integrated mass spectrometry imaging and digital pathology workflow for objective detection of colorectal tumours by unique atomic signatures

“…On average, a 40 × 40 μm laser spot produces images of colorectal resections with approximately 200 000 pixels per measured m / z , with sufficient signal-to-noise ratios for up to eight mid-mass range elements with a scan cycle time of 0.25 s. We experimented with 6–8 element panels with m / z ranging from 13 to 66, and a conservative laser scan speed of 160 μm s −1 , and acknowledge that future clinical translation work will require the use of new hardware advances 38 that eliminate laser and ICP-MS syncing issues and aliasing effects for statistically robust high throughput imaging. 39 Pixels falling within these set x – y coordinates were extracted in Igor Pro (see Methods) using the annotated H&E for later statistical analyses and comparisons.…”

Atomic spectrometry update: review of advances in atomic spectrometry and related techniques

“…Fine-tuning of operating conditions was achieved using the principle of divisors. 59 This involved nding instrumental conditions to satisfy the antialiasing criterion, i.e., repetition rate x acquisition time ¼ Z + . The mathematics involved was developed as an app (https:// laicpms-apps.ki.si/webapps/home/).…”

Laser Ablation–Inductively Coupled Plasma–Mass Spectrometry Imaging in Biology

“…In a follow-up study, they discussed the fine-tuning of system-wide parameters for high-speed and high-resolution imaging and developed a mathematical model for selection of instrumental conditions to minimize image blur and aliasing while optimizing the signal-to-noise ratios. The model is integrated in an open-source web-app for newcomers in LA-ICP-MS imaging …”