Fine Tuning Genetic Circuits via Host Context and RBS Modulation
Dennis Tin Chat Chan,
Lena Winter,
Johan Bjerg
et al.
Abstract:SUMMARYThe choice of organism to host a genetic circuit – the chassis – is often defaulted to model organisms due to their amenability. The chassis-design space has therefore remained underexplored as an engineering variable. In this work, we explored the design space of a genetic toggle switch through variations in nine ribosome binding sites compositions and three host contexts, creating 27 circuit variants. Characterization of performance metrics in terms of toggle switch output and host growth dynamics unv… Show more
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