Fine-tuned dehydration by trehalose enables the cryopreservation of RBCs with unusually low concentrations of glycerol

Shen, Lingxiao; Guo, Xiaojie; Ouyang, Xilin; Huang, Yu Chuen; Gao, Dayong; Zhao, Gang

doi:10.1039/d0tb02426k

Cited by 27 publications

(54 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the RBCs’ recovery in the glycerol group was only 74.9 ± 2.6%, significantly lower than that in any other group ( p < 0.001). The result is consistent with the conclusion that high concentrations of glycerol easily cause hemolysis [ 15 ]. HES is often used as a plasma substitute, indicating its good safety for blood [ 27 ].…”

Section: Resultssupporting

confidence: 92%

“…Glycerol is the only CPA that has been approved for the clinical cryopreservation of RBCs [ 14 ]. However, to obtain satisfactory cell recovery, very high concentrations of glycerol must be added (20–40% w / v ) [ 15 ]. Such high concentrations of glycerol induce the hemolysis of RBCs [ 16 ], and the procedure of deglycerolization after cryopreservation can be very complex [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tricine as a Novel Cryoprotectant with Osmotic Regulation, Ice Recrystallization Inhibition and Antioxidant Properties for Cryopreservation of Red Blood Cells

Liu

Zhang

et al. 2022

IJMS

View full text Add to dashboard Cite

The cryopreservation of red blood cells (RBCs) plays a key role in blood transfusion therapy. Traditional cryoprotectants (CPAs) are mostly organic solvents and may cause side effects to RBCs, such as hemolysis and membrane damage. Therefore, it is necessary to find CPAs with a better performance and lower toxicity. Herein, we report for the first time that N-[Tri(hydroxymethyl)methyl]glycine (tricine) showed a great potential in the cryopreservation of sheep RBCs. The addition of tricine significantly increased the thawed RBCs’ recovery from 19.5 ± 1.8% to 81.2 ± 8.5%. The properties of thawed RBCs were also maintained normally. Through mathematical modeling analysis, tricine showed a great efficiency in cryopreservation. We found that tricine had a good osmotic regulation capacity, which could mitigate the dehydration of RBCs during cryopreservation. In addition, tricine inhibited ice recrystallization, thereby decreasing the mechanical damage from ice. Tricine could also reduce oxidative damage during freezing and thawing by scavenging reactive oxygen species (ROS) and maintaining the activities of endogenous antioxidant enzymes. This work is expected to open up a new path for the study of novel CPAs and promote the development of cryopreservation of RBCs.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Tricine as a Novel Cryoprotectant with Osmotic Regulation, Ice Recrystallization Inhibition and Antioxidant Properties for Cryopreservation of Red Blood Cells

Liu

Zhang

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“… 19 Yet, due to this noncell penetrating property, trehalose is highly tolerated by humans and can be administered without being removed after thawing. 20 …”

Section: Introductionmentioning

confidence: 99%

Sugar-Assisted Cryopreservation of Stem Cell-Laden Gellan Gum–Collagen Interpenetrating Network Hydrogels

Tan

2022

Biomacromolecules

View full text Add to dashboard Cite

Tissue engineering involves the transplantation of stem cell-laden hydrogels as synthetic constructs to replace damaged tissues. However, their time-consuming fabrication procedures are hurdles to widespread application in clinics. Fortunately, similar to cell banking, synthetic tissues could be cryopreserved for subsequent central distribution. Here, we report the use of trehalose and gellan gum as biomacromolecules to form a cryopreservable yet directly implantable hydrogel system for adipose-derived stem cell (ADSC) delivery. Through a modified cell encapsulation method and a preincubation step, adequate cryoprotection was afforded at 0.75 M trehalose to the encapsulated ADSCs. At this concentration, trehalose demonstrated lower propensity to induce apoptosis than 10% DMSO, the current gold standard cryoprotectant. Moreover, when cultured along with trehalose after thawing, the encapsulated ADSCs retained their stem cell-like phenotype and osteogenic differentiation capacity. Taken together, this study demonstrates the feasibility of an “off-the-shelf” biomacromolecule-based synthetic tissue to be applied in widespread tissue engineering applications.

show abstract

“…Recently, the cryopreservation of blood has become an urgent need because of the sizable demand and the short survival time (42 days at most) without cryopreservation and in complex isotonic solutions. − According to previous reports, the extracellular ice formation (ice recrystallization) during the thawing process is one of the major reasons causing cell death. In order to solve this problem, a lot of synthetic materials such as glycopeptides, peptides, polymers, , hydrogel microfiber, and some other nano materials − with specific ice recrystallization inhibition (IRI) activity and a good cryoprotective effect have been reported. However, the toxicity and biodegradability of these synthetic materials remain to be investigated.…”

Section: Introductionmentioning

confidence: 99%

Nonionic and Water-Soluble Poly(d/l-serine) as a Promising Biomedical Polymer for Cryopreservation

Sun

Liu

et al. 2021

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Water-soluble, biodegradable, nonionic, and biocompatible polymers with multiple functional groups are highly desired for biomedical applications. Here, we report that water-soluble nonionic poly(d/l-serine) is chirality-controllable, biodegradation-controllable, and non-cytotoxic. Hence, it can be a highly sought-after alternative to the widely used poly(ethylene glycol), with an additional advantage of having multiple hydroxyl groups for further functionalization. As one example of its biomedical applications, poly(d/l-serine) demonstrated an obvious cryoprotective effect on the red blood cells. The usage of poly(d/l-serine) in the cryopreservation field would be of great promise to resolve the difficulties in separating cryoprotectants due to toxicity.

show abstract

Fine-tuned dehydration by trehalose enables the cryopreservation of RBCs with unusually low concentrations of glycerol

Abstract: We regulated the amount of trehalose and combined it with glycerol to achieve unusually low glycerol concentrations in the cryopreservation of RBCs compared with traditional methods.

Cited by 27 publications

References 54 publications

Tricine as a Novel Cryoprotectant with Osmotic Regulation, Ice Recrystallization Inhibition and Antioxidant Properties for Cryopreservation of Red Blood Cells

Tricine as a Novel Cryoprotectant with Osmotic Regulation, Ice Recrystallization Inhibition and Antioxidant Properties for Cryopreservation of Red Blood Cells

Sugar-Assisted Cryopreservation of Stem Cell-Laden Gellan Gum–Collagen Interpenetrating Network Hydrogels

Nonionic and Water-Soluble Poly(d/l-serine) as a Promising Biomedical Polymer for Cryopreservation

Contact Info

Product

Resources

About