Fine structure of zinnia leaf tissues infected with dahlia mosaic virus

Kitajima, E. W.; Lauritis, J. A.; Swift, Hewson

doi:10.1016/0042-6822(69)90044-0

Cited by 46 publications

(17 citation statements)

References 15 publications

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“…4F and G and Fig. 5E), as also reported earlier by others (35,36). This suggests that virions do not diffuse freely in the cytoplasm in the absence of stress.…”

Section: Discussionsupporting

confidence: 74%

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Virus Factories of Cauliflower Mosaic Virus Are Virion Reservoirs That Engage Actively in Vector Transmission

Bak

Gargani

Macia

et al. 2013

J Virol

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cCauliflower mosaic virus (CaMV) forms two types of inclusion bodies within infected plant cells: numerous virus factories, which are the sites for viral replication and virion assembly, and a single transmission body (TB), which is specialized for virus transmission by aphid vectors. The TB reacts within seconds to aphid feeding on the host plant by total disruption and redistribution of its principal component, the viral transmission helper protein P2, onto microtubules throughout the cell. At the same time, virions also associate with microtubules. This redistribution of P2 and virions facilitates transmission and is reversible; the TB reforms within minutes after vector departure. Although some virions are present in the TB before disruption, their subsequent massive accumulation on the microtubule network suggests that they also are released from virus factories. Using drug treatments, mutant viruses, and exogenous supply of viral components to infected protoplasts, we show that virions can rapidly exit virus factories and, once in the cytoplasm, accumulate together with the helper protein P2 on the microtubule network. Moreover, we show that during reversion of this phenomenon, virions from the microtubule network can either be incorporated into the reverted TB or return to the virus factories. Our results suggest that CaMV factories are dynamic structures that participate in vector transmission by controlled release and uptake of virions during TB reaction.

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“…4F and G and Fig. 5E), as also reported earlier by others (35,36). This suggests that virions do not diffuse freely in the cytoplasm in the absence of stress.…”

Section: Discussionsupporting

confidence: 74%

“…In contrast to TBs, which are dynamic structures throughout infection, mature VFs are believed to exchange none or little of their contents with the cytoplasm (35,36). We wanted to know whether this is true or whether mature VFs can release significant amounts of virions, at least transiently.…”

Section: Resultsmentioning

confidence: 99%

Virus Factories of Cauliflower Mosaic Virus Are Virion Reservoirs That Engage Actively in Vector Transmission

Bak

Gargani

Macia

et al. 2013

J Virol

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“…SPCV, however, failed to react with antisera to three commonly occurring caulimoviruses (cauliflower mosaic, dahlia mosaic and carnation etched ring viruses), and was not transmitted in the semi-persistent manner by aphids (Myzus persicae and Aphis gossypii); moreover, its particles occur abundantly in cytoplasm but not within inclusions which are structurally distinct from those of typical caulimoviruses (e.g. KITAJIMA et al 1969, BRUNT and KITAJIMA 1973, SHEPHERD and LAWSON 1981, HULL 1984. Because the affinities of SPCV are uncertain, the virus and its protein and nucleic acid components are now being further characterised.…”

Section: Resultsmentioning

confidence: 99%

Electron Microscopy of an Isometric Caulimo‐Like Virus from Sweet Potato (Ipomoea batatas)

Atkey¹,

Brunt²

1987

Journal of Phytopathology

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A newly recognised virus with isometric particles 50 nm in diameter was detected in Ipomoea batatas (sweet potato) by graft-transmission to /. setosa. Virus particles and intracellular inclusions, although not seen in infected /. batatus, were found in the cytoplasm of most cell types of /. setosa. The caulimo-Iike particles were most abundant with vesicles immediately adjacent to inclusions. The ovoid or spherical inclusions, which differ markedly from those of caulimoviruses, have a large central lacuna and usually several smaller peripheral lacunae. Infected vascular parenchyma cells sometimes protrude into, and occasionally completely occlude, adjacent xylem vessels, observations possibly explaining the sudden wilting and premature senescence of infected leaves.

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“…Although they could be a normal constituent, or a virus parasite, of P. brassicae cells, this seems unlikely since they do not occur in gall plasmodia (43). Another possibility is suggested by the resemblance of individual inclusion bodies to virions of brassica virus 3 and dahlia mosaic virus (27,38). However, since the unidentified inclusion bodies are not associated with viroplasm, often occur in paracrystalline array, and are sometimes found in nuclei, it seems unlikely that they are virions of a known cabbage virus.…”

Section: Discussionmentioning

confidence: 96%

The cytology and kinetics of cabbage root hair penetration by Plasmodiophora brassicae

Aist¹,

Williams²

1971

Can. J. Bot.

170

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1971. The cytology and kinetics of cabbage root hair penetration by Plasmodiophora brassicae. Can. J. Bot. 49: 2023-2034. After a primary zoospore of Plasnlodiophora brassicae attaches to a cabbage root hair wall, the flagella coil, and the axonemes are soon retracted. During the next 2 h a dense-staining bullet-like Stachel is formed within a tubular cavity in the cyst, the Rohr. Possibly because of pressure created within an expanding cyst vacuole, the Rohr is quickly evaginated to form a bulbous adhesorium. The Stachel punctures the host wall and then, in a period of about 1 s, the parasite is injected into the root hair. The total time required for adhesorium formation and host penetration is about 1 min. Secondary zoospores were found to be capable of carrying out a similar penetration process. After penetration a callose-rich papilla is soon formed between the host wall and plasma membrane at the penetration site. Young amoebae in root hairs have no lipid bodies and are surrounded by seven-layered envelopes. Unidentified spherical inclusion bodies were seen sporadically in primary zoospores and cysts, and in uninucleate amoebae.

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Fine structure of zinnia leaf tissues infected with dahlia mosaic virus

Cited by 46 publications

References 15 publications

Virus Factories of Cauliflower Mosaic Virus Are Virion Reservoirs That Engage Actively in Vector Transmission

Virus Factories of Cauliflower Mosaic Virus Are Virion Reservoirs That Engage Actively in Vector Transmission

Electron Microscopy of an Isometric Caulimo‐Like Virus from Sweet Potato (Ipomoea batatas)

The cytology and kinetics of cabbage root hair penetration by Plasmodiophora brassicae

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