Fine structure of vascular endothelium in culture

Haudenschild, Christian C.; Cotran, Ramzi S.; Gimbrone, Michael A.; Folkman, Judah

doi:10.1016/s0022-5320(75)90004-0

Cited by 174 publications

(48 citation statements)

References 23 publications

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“…The cells cultured from human umbilical cords were examined by light and electron microscopy. Their morphology was similar to that described by others for cultured endothelium (17)(18)(19)33). The starting cultures were compact colonies of hexagonal cells that spread into monolayers at a relatively slow rate.…”

Section: Resultssupporting

confidence: 70%

Metabolism of vasoactive peptides by human endothelial cells in culture. Angiotensin I converting enzyme (kininase II) and angiotensinase.

Johnson¹,

Erdös²

1977

J. Clin. Invest.

189

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Section: Resultssupporting

confidence: 70%

Metabolism of vasoactive peptides by human endothelial cells in culture. Angiotensin I converting enzyme (kininase II) and angiotensinase.

Johnson¹,

Erdös²

1977

J. Clin. Invest.

189

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“…The cultures, however, were not pure, as the occasional smooth muscle cell was seen, and a proportion (15-20%) of the cells may have been endothelial in origin (Jaffe et at., 1973;Kawamura et al, 1974;Haudenschild et al, 1975), though the characteristic rod-shaped cytoplasmic inclusions first described by Weibel and Palade (1964) were not seen.…”

Section: Identity Of Cellsmentioning

confidence: 96%

Effects of dexamethasone and betamethasone on in vitro cultures from human astrocytoma

Guner¹,

Freshney²,

Morgan³

et al. 1977

Br J Cancer

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Summary.-Cultures of human astrocytoma have been derived by collagenase digestion and are presumed, from their aneuploid karyotypes, to be predominantly neoplastic. Early passage cultures in proliferative phase have been cloned in the presence of dexamethasone and betamethasone, both commonly used in management of patients with brain tumours. These steroids raise both the cloning efficiency and the proliferative capacity of cells within each clone. Inhibition was detected only in very high steroid concentrations (25-50 ,ug/ml). Since these concentrations are unlikely to be attained in vivo it is concluded that anticipated physiological levels of these steroids enhance cell survival at low densities in culture. The significance of this in vivo is discussed.

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“…580000 cells/well) were used for the experiments described below. The cells were identified äs endothelial cells by the typical cobblestone, contact-inhibited morphology (13) and by factor VIII (14) staining. · ' >…”

Section: Cell Culturementioning

confidence: 99%

In Vitro Effects of Hypoxia and Reoxygenation on Human Umbilical Endothelial Cells

Windischbauer

Griesmacher²,

Müller³

1994

CCLM

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Summary:We investigated metabolic changes in human umbilical venous endothelial cells, when these were incubated under hypoxic followed by hyperoxic conditions, thus simulating hypoxia and reoxygenation..The human umbilical venous endothelial cells were incubated with a degassed buffer (oxygen content: 0-0.5%) for either 3 h or 24h, followed by a 60min incubation with oxygen-perfused buffer (oxygen content: 100%). Three hours of hypoxia led to a slight decrease in the ATP and creatine phosphate content (-16% ± 5%), while a pronounced decrease of high energy phosphates (-54% ± 4%) was observed after 24 h of hypoxia. Reoxygenating the cells after 3 h of hypoxia led to restoration of the content of high energy phosphates, while reoxygenation after 24 h resulted in a strong decrease (-66% ± 4%). The prostaglandin I 2 release during the first 3 h of hypoxia exceeded the release in the following 21 h. In all cases, reoxygenation increased the prostaglandin I 2 release. Under normoxic conditions the ratio between oxidised glutathione and reduced glutathione shifted from l : 100 to l : 4.5 after 3 h of hypoxia. The content of lipid peroxidation products was almost unaffected during hypoxia, whereas reoxygenation resulted in a pronounced increase (+ 380% ± 60%). The results of this in vitro study suggest that relatively long periods of hypoxia lead to a deficiency of high energy phosphates in the cell. Reoxygenation leads to the formation of oxygen-derived radicals, irrespectively of a prior hypoxia.

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Fine structure of vascular endothelium in culture

Cited by 174 publications

References 23 publications

Metabolism of vasoactive peptides by human endothelial cells in culture. Angiotensin I converting enzyme (kininase II) and angiotensinase.

Metabolism of vasoactive peptides by human endothelial cells in culture. Angiotensin I converting enzyme (kininase II) and angiotensinase.

Effects of dexamethasone and betamethasone on in vitro cultures from human astrocytoma

In Vitro Effects of Hypoxia and Reoxygenation on Human Umbilical Endothelial Cells

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