Fine structure of the lymphatic capillary and the adjoining connective tissue area

Leak, Lee V.; Jf, Burke

doi:10.1002/aja.1001180308

Cited by 243 publications

(121 citation statements)

References 46 publications

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“…1, 2, 3, and 4, respectively. In all instances the intima consisted of an unbroken endothelium displaying ultrastructural features now recognized as characteristic oflymphatic endothelial cells (1,2,20,37,39), including an attenuated basal lamina.…”

Section: Integrity Of Perfused Lymphaticsmentioning

confidence: 99%

“…Regardless of its relative importance in lymph formation, a question subject to much debate, intercellular transport in vivo is heavily dependent upon adluminally directed convective forces (11,20,21). The ultrastructural analyses of the present study showed no essential differences in any intercellular contact-related variable among the groups.…”

Section: Interendothelial Contactsmentioning

confidence: 99%

“…INTER ENDOTHELIAL CONTACTS : The frequency of the three forms of intercellular contacts-end-to-end, overlapping, and interdigitating-as well as the incidence of so-called "open" (>30 nm wide) junctions (11,20,21) was manually recorded as the perimeter of each vessel was scanned with the electron microscope. Each contact was further characterized by whether it revealed a specialized form of junction .…”

Section: Morphometrymentioning

confidence: 99%

“…However, the pathways taken and the forces that control this fluid movement are poorly understood and therefore have become the subjects of considerable controversy. According to one theory the major pathway lies between adjacent endothelial cells (11,20,21), and hydrostatic (16) or osmotic pressure (11, 12) provides the necessary force for fluid transport. Integral to this theory are the cyclic changes that occur because lymphatic vessels are alternately compressed and then relaxed within the tissues.…”

mentioning

confidence: 99%

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Temperature dependence of protein transport across lymphatic endothelium in vitro.

O’Morchoe¹,

Jones²,

Jarosz³

et al. 1984

The Journal of Cell Biology

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The purpose of the work was to develop an in vitro model for the study of lymphatic endothelium and to determine, using this model, whether or not a cytoplasmic process may be involved in transendothelial transport . Segments of canine renal hilar lymphatics were dissected clean, cannulated at both ends, and transferred to a perfusion chamber for measurement of transendothelial protein transport and for ultrastructural tracer studies . The segments were subsequently processed for light and electron microscopy. By both structural and functional criteria the lymphatics were judged to have retained their integrity. At 37°C, 36 lymphatics showed a mean rate of protein transport of 3 .51 ± 0 .45 (SEM) ug/min per cm' of lymphatic endothelium . The rate was influenced by the temperature of the system, being significantly reduced by 49% ± 4.8, 31% ± 5.3, and 29% ± 3 .9 when the temperature was lowered to 4°, 24°, and 30°C, respectively . When the temperature was raised to 40°C, the rate was significantly increased by 48% ± 12.2. The vesicular system and the intercellular regions in vessels with increased or reduced rates of transport were analyzed quantitatively to ascertain whether the rate changes could be correlated with ultrastructurally demonstrable changes in either of these postulated pathways . No significant changes in junctional or vesicular parameters were found between the control lymphatics and those perfused at 24°, 30°, and 40°C . At 4°C, the temperature at which the rate of protein transport was maximally reduced, vesicular size decreased, and the number of free cytoplasmic vesicles increased, whereas the number associated with the abluminal and luminal surfaces decreased . We concluded that isolated perfused lymphatic segments transport protein at a relatively constant rate under control conditions, and that this transendothelial transport comprises both temperaturedependent and temperature-independent mechanisms . The findings were considered in terms of the different theories of lymph formation and were interpreted as providing support for the vesicular theory .Lymph, by definition, is formed from that component of the interstitial fluid that moves across the endothelium of lymphatic vessels. However, the pathways taken and the forces that control this fluid movement are poorly understood and therefore have become the subjects of considerable controversy. According to one theory the major pathway lies between adjacent endothelial cells (11,20,21), and hydrostatic (16) or osmotic pressure (11, 12) provides the necessary force for fluid transport. Integral to this theory are the cyclic changes that occur because lymphatic vessels are alternately compressed and then relaxed within the tissues. For instance, if hydrostatic pressure is the major force, intraluminal pressure THE JOURNAL OF CELL BIOLOGY -VOLUME 98 FEBRUARY 1984 629-640 0 The Rockefeller University Press -0021-9525/84/02/0629/12 $1 .00 must be greater than atmospheric attimes, thus causing lymph to flow downstream, and less than atmo...

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Section: Integrity Of Perfused Lymphaticsmentioning

confidence: 99%

Section: Interendothelial Contactsmentioning

confidence: 99%

Section: Morphometrymentioning

confidence: 99%

mentioning

confidence: 99%

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Temperature dependence of protein transport across lymphatic endothelium in vitro.

O’Morchoe¹,

Jones²,

Jarosz³

et al. 1984

The Journal of Cell Biology

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“…The latter is also due to the contraction of surrounding skeletal muscles, as well as arterial pulsations. Like veins, the larger lymphatic vessels contain one-way intraluminal valves that aid in lymph propulsion by preventing backXow (Leak and Burke 1966;von der Weid and Zawieja 2004). Compared to blood vessels, the lymphatic vasculature is a low Xow and low pressure system.…”

Section: Introductionmentioning

confidence: 99%

Developmental and pathological lymphangiogenesis: from models to human disease

Hajjami

Petrova

2008

Histochem Cell Biol

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The lymphatic vascular system, the body's second vascular system present in vertebrates, has emerged in recent years as a crucial player in normal and pathological processes. It participates in the maintenance of normal tissue Xuid balance, the immune functions of cellular and antigen traYcking and absorption of fatty acids and lipidsoluble vitamins in the gut. Recent scientiWc discoveries have highlighted the role of lymphatic system in a number of pathologic conditions, including lymphedema, inXammatory diseases, and tumor metastasis. Development of genetically modiWed animal models, identiWcation of lymphatic endothelial speciWc markers and regulators coupled with technological advances such as high-resolution imaging and genome-wide approaches have been instrumental in understanding the major steps controling growth and remodeling of lymphatic vessels. This review highlights the recent insights and developments in the Weld of lymphatic vascular biology.

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Lymphatics of the Atrioventricular Valves

Bradham¹

1973

Arch Surg

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Fine structure of the lymphatic capillary and the adjoining connective tissue area

Cited by 243 publications

References 46 publications

Temperature dependence of protein transport across lymphatic endothelium in vitro.

Temperature dependence of protein transport across lymphatic endothelium in vitro.

Developmental and pathological lymphangiogenesis: from models to human disease

Lymphatics of the Atrioventricular Valves

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