Fine Structure of the Developing Avian Corneal Stroma as Revealed by Quick-freeze, Deep-etch Electron Microscopy

Hirsch, M.; Noske, W; Prenant, G.; Renard, G

doi:10.1006/exer.1999.0695

Cited by 28 publications

(15 citation statements)

References 41 publications

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“…diameter) of corneal collagen fibrils in chicks during morphogenesis [39,40], and our electronmicroscopic findings concur. This suggests that changes in sulphated PGs have no appreciable effect on the ultimate size to which collagen fibrils grow in the developing chick cornea.…”

Section: Discussionsupporting

confidence: 76%

Proteoglycan Alterations and Collagen Reorganisation in the Secondary Avian Cornea during Development

Connon

Siegler²,

Meek³

et al. 2003

Ophthalmic Res

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Purpose: It is thought that proteoglycan (PG) alterations, collagen matrix reorganisation and the onset of corneal transparency in the developing avian cornea might be related events. The current histochemical study was designed to establish the character and distribution of corneal PG filaments in relation to collagen organisation during tissue morphogenesis. Methods: Corneas from days 13–18 developing chicken embryos were treated with cuprolinic blue (CuB) to examine sulphated PGs by transmission electron microscopy and quantitative image analysis. Results: On developmental day 13, corneas contained poorly defined lamellae and a large number of both small and large CuB-stained PG filaments, randomly distributed and often in collagen-free regions. By day 14 and after, the large CuB-stained PG filaments were much less abundant. At this time, too, collagen fibrils displayed an axial alignment and an occasional periodic arrangement of small CuB-stained PG filaments along their axes. By developmental day 15, lamellae were well formed and continued to increase in number and size thereafter. Between developmental days 16 and 17, there was a significant increase (p < 0.001) in the number of small, collagen-associated PG filaments. This increase persisted into day 18. Conclusions: The size, number and distribution of sulphated, CuB-stained PG filaments in the developing avian cornea change over time. This is particularly true between developmental days 13 and 14 and between days 16 and 17, concurrent with previously documented structural changes.

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Section: Discussionsupporting

confidence: 76%

Proteoglycan Alterations and Collagen Reorganisation in the Secondary Avian Cornea during Development

Connon

Siegler²,

Meek³

et al. 2003

Ophthalmic Res

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“…The established model for the study of corneal development is the chicken eye, in which corneal morphogenesis is dominated by well documented structural transformations that govern tissue form and function (Linsenmayer et al, 1998). Light and electron microscopy have revealed that from embryonic day 12 to hatch at day 21 a condensation takes place during which time the tissue thins and the collagen fibrillar array becomes remodelled (Birk & Trelstad, 1984;Linsenmayer et al, 1990;Hirsch et al, 1999). Also, around this time the cornea becomes transparent, with early spectrophotometry measurements showing that at developmental day 14 only about 40% of incident white light is transmitted, whereas by day 18 transmission is over 90%, close to adult levels (Coulombre & Coulombre, 1958).…”

Section: Developmental Eventsmentioning

confidence: 99%

Small-angle fibre diffraction studies of corneal matrix structure: a depth-profiled investigation of the human eye-bank cornea

et al. 2007

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In the cornea of the eye light transmission is facilitated by the regular arrangement and uniform diameter of collagen fibrils that constitute the bulk of the extracellular corneal matrix. Matrix architecture, in turn, is believed to be governed by interactions between collagen fibrils and proteoglycan molecules modified with sulfated glycosaminoglycan side chains. Here, we outline the contribution made by small‐angle X‐ray scattering studies of the cornea in understanding the role of sulfated glycosaminoglycans in the control of collagen architecture in cornea, and present new depth‐profiled microbeam data from swollen human eye‐bank corneas that indicate no significant change in collagen fibril diameter throughout the tissue, but a lower collagen interfibrillar spacing in the anterior‐most stromal regions compared with the ultrastructure of the deeper cornea.

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“…Equivalent structures have recently been revealed in interfacial regions and in close association with stromal cells in avian cornea (Hirsch et al, 1999a). That suggests a role in matrix±matrix and cell±matrix interactions.…”

Section: Discussionmentioning

confidence: 98%

“…All these samples were mounted posterior side up on gold specimen holders (Balzers, Liechtenstein) using 2 % gelatin as adhesive, blotted with ®lter paper to remove excess¯uid and processed for ultrarapid-freezing as previously described (Hirsch et al, 1999a). Brie¯y, mounted samples were ultrarapidly-frozen by slamming and pressing them against an ultrapure copper block cooled to À2608C with liquid helium in a Cryobloc apparatus (Reichert±Jung, France) or to À1758C with liquid nitrogen in a LifeCell CF 100 2 quickfreezing machine (LifeCell Corporation, The Woodlands, TX, U.S.A.).…”

Section: Ultrarapid-freezingmentioning

confidence: 99%

“…Its application to the corneal stroma ECM is itself a recent development (Hirsch et al, 1989(Hirsch et al, , 1992(Hirsch et al, , 1999bYamabayashi et al, 1991;Meller, Spitznas and Meller, 1997). In our most recent study, we used this method to analyse the ®ne structure of avian corneal stromal ECM during morphogenesis (Hirsch et al, 1999a). We were able to distinguish different ultrastructural pro®les and motifs, which we tentatively identi®ed as ECM components transiently or continually expressed in this tissue.…”

Section: Introductionmentioning

confidence: 97%

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Three-dimensional Supramolecular Organization of the Extracellular Matrix in Human and Rabbit Corneal Stroma, as Revealed by Ultrarapid-freezing and Deep-etching Methods

Hirsch

Prenant

Renard

2001

Experimental Eye Research

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Fine Structure of the Developing Avian Corneal Stroma as Revealed by Quick-freeze, Deep-etch Electron Microscopy

Cited by 28 publications

References 41 publications

Proteoglycan Alterations and Collagen Reorganisation in the Secondary Avian Cornea during Development

Proteoglycan Alterations and Collagen Reorganisation in the Secondary Avian Cornea during Development

Small-angle fibre diffraction studies of corneal matrix structure: a depth-profiled investigation of the human eye-bank cornea

Three-dimensional Supramolecular Organization of the Extracellular Matrix in Human and Rabbit Corneal Stroma, as Revealed by Ultrarapid-freezing and Deep-etching Methods

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