Fine Structure of Methane‐utilizing Bacteria

Proctor, Heather M.; Norris, J. R.; Ribbons, Douglas W.

doi:10.1111/j.1365-2672.1969.tb02196.x

Cited by 44 publications

(19 citation statements)

References 14 publications

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“…116 MICROBIAL METABOLISM OF METHANE 639 see whether the particulate nature of the enzyme system catalysing the condensation offormaldehyde with pentose phosphate in P. methanica and Mtl. cap8ulatu8, as found in the present study, can be correlated with the elaborate internal membrane system found in methane-utilizing bacteria (Whittenbury, 1969;Proctor, Norris & Ribbons, 1969 …”

Section: Discussionmentioning

confidence: 70%

Synthesis of cell constituents by methane-grown Methylococcus capsulatus and Methanomonas methanooxidans

Lawrence

Kemp

Quayle

1970

Biochemical Journal

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1. A study was made of the incorporation of carbon from [(14)C]methanol by cultures of Methylococcus capsulatus and Methanomonas methanooxidans growing on methane. 2. The distribution of radioactivity within the non-volatile constituents of the ethanol-soluble fractions of the cells, after incubation with labelled substrate for periods of up to 3min, was analysed by chromatography and radioautography. 3. Over 80% of the radioactivity fixed by Methylococcus capsulatus at 30 degrees C at the earliest times of sampling appeared in phosphorylated compounds, of which glucose phosphate constituted 60%. 4. Most of the radioactivity fixed by Methanomonas methanooxidans at 30 degrees C at the earliest times of sampling appeared in serine, malate, aspartate and an unknown compound(s) tentatively suggested to be folate derivative(s). At 16 degrees C, [(14)C]methanol was fixed predominantly into serine and the unknown compound(s). 5. Extracts of Methylococcus capsulatus contain an enzyme system that catalyses the condensation of formaldehyde and ribose 5-phosphate to give a mixture consisting mainly of fructose phosphate and allulose phosphate. No similar activity was detected in extracts of Methanomonas methanooxidans. A convenient method was developed for assay of this enzyme system. 6. The enzyme system catalysing the condensation of formaldehyde with ribose 5-phosphate is particle-bound in both Methylococcus capsulatus and Pseudomonas methanica and is unstable in the absence of Mg(2+). 7. Extracts of Methanomonas methanooxidans contain high activities of d-glycerate-NAD oxidoreductase, whereas extracts of Methylococcus capsulatus and Pseudomonas methanica contain negligible activities of this enzyme. 8. These results indicate that during growth of Methylococcus capsulatus on methane, as with Pseudomonas methanica, cell constituents are made by the ribose phosphate cycle of formaldehyde fixation. This contrasts with Methanomonas methanooxidans, whose assimilation pathway resembles in some features that of Pseudomonas AM1 growing on methanol.

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Section: Discussionmentioning

confidence: 70%

Synthesis of cell constituents by methane-grown Methylococcus capsulatus and Methanomonas methanooxidans

Lawrence

Kemp

Quayle

1970

Biochemical Journal

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“…The isolates of Whittenbury and colleagues, as well as the previous isolates, have all had an obligate requirement for methane, methanol, or dimethylether as the growth substrate. All of these methane-utilizing bacteria so far examined possess elaborate internal membrane structures when grown on methane (9,10,29,31,38,39,46). When methane utilizers are grown on methanol these internal membranes are also present, but to a lesser extent (10,38).…”

mentioning

confidence: 99%

Intracytoplasmic membrane, phospholipid, and sterol content of Methylobacterium organophilum cells grown under different conditions

Patt¹,

Hanson²

1978

J Bacteriol

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Intracytoplasmic membranes were present in Methylobacterium organophilum when cells were grown with methane, but not methanol or glucose, as the sole carbon and energy source. Cells grown with methane as the carbon and energy source and low levels of dissolved oxygen had the greatest amount of intracytoplasmic membrane. Cells grown with increased levels of dissolved oxygen had less intracytoplasmic membrane. The amount of total lipid correlated with the amount of membrane material observed in thin sections. The individual phospholipids varied in amount, but the same four were present in M. organophilum grown with different substrates and oxygen levels. Phosphatidyl choline was present as a major component of the phospholipids. Sterols were present, and they differed from those in the type I methylotroph Methylococcus capsulatus. The relative amounts of different sterols and squalene changed with the substrate provided for growth. The greatest amounts of sterols were found in methane-grown cells grown at low levels of dissolved oxygen. None of the unusual or usual membrane components assayed was uniquely present in the intracytoplasmic membranes.

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“…partikelgebundenes Enzym handelt. Bekannt war zu diesem Zeitpunkt schon, daB Methan utilisierende Bakterien ein besonderes internes Membransystem besitzen (PROCTOR et al 1969, DAVIES u. WHITTENBURY 1970). So ist es nicht verwunderlich, daB sie dieses Membransystem mit dem Enzym, das sich auBerdem im Zellhoniogenat als labil erwies, in Zusammenhang brachten.…”

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Kinetik der Hexulose‐6‐phosphat‐Synthase methylotropher Bakterien in vitro und in situ

Babel¹,

Müller²

1977

J of Basic Microbiology

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Fine Structure of Methane‐utilizing Bacteria

Cited by 44 publications

References 14 publications

Synthesis of cell constituents by methane-grown Methylococcus capsulatus and Methanomonas methanooxidans

Synthesis of cell constituents by methane-grown Methylococcus capsulatus and Methanomonas methanooxidans

Intracytoplasmic membrane, phospholipid, and sterol content of Methylobacterium organophilum cells grown under different conditions

Kinetik der Hexulose‐6‐phosphat‐Synthase methylotropher Bakterien in vitro und in situ

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