Fine structure of fibroblasts in the periodontal ligament of the rat incisor and their possible role in tooth eruption

Beertsen, W.; Everts, Vincent; Hooff, A. van den

doi:10.1016/0003-9969(74)90235-0

Cited by 146 publications

(50 citation statements)

References 35 publications

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“…The periostin peptide was uniformly localized in the lingual periodontal ligament, including both the tooth half and alveolar half of the ligament, which were termed the tooth-and alveolus-related parts, respectively ( Fig. 3E) (Beertsen et al, 1974). These immunoreactions exclusively appeared as the filamentous elements in the periodontal ligament.…”

Section: Periostin In Mandibles Of 7-day-old Micementioning

confidence: 95%

Immunohistochemical localization of periostin in tooth and its surrounding tissues in mouse mandibles during development

et al. 2004

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Previous reports have shown expression of immunoreactivity for periostin, originally identified as osteoblastspecific factor-2, in the periosteum and periodontal ligament. However, the developmental changes in its expression and the detailed immunolocalization have remained veiled. The present study was undertaken to examine the spatiotemporal expression of this protein in teeth and their associated tissues of mice during development at light and electron microscopic levels. In tooth germs at cap stage, periostin immunoreactivity was recognizable in the interface between inner enamel epithelium and preodontoblasts as well as in the mesenchymal tissues around cervical loop. Dental follicles around tooth germs at bell stage localized periostin immunopositivity in addition to the immunopositive areas observed in cap-staged tooth germs, although the functional significance of periostin has remained unclear in tooth development. Furthermore, periostin immunoreactivity was also found in the alveolar bone surface. In the incisors of both 7-and 21-day-old mice, immunoreaction for periostin was discernible in the lingual periodontal ligament and labial fibrous tissue adjacent to the papillary layer. After postnatal day 7, immunoreaction for periostin came to be restricted to the fibrous bundles in the periodontal ligament in accordance with the organization of the periodontal fibers, indicating its localization matched the morphogenesis of the periodontal ligament. Immunoelectron microscopic observation of the mature periodontal ligament verified the localization of periostin between the cytoplasmic processes of periodontal fibroblasts and cementoblasts and the adjacent collagen fibrils. Our findings suggest that periostin is involved at the sites of the cell-to-matrix interaction, serving as adhesive equipment for bearing mechanical forces, including occlusal force and tooth eruption.

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Section: Periostin In Mandibles Of 7-day-old Micementioning

confidence: 95%

Immunohistochemical localization of periostin in tooth and its surrounding tissues in mouse mandibles during development

et al. 2004

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“…Some of the flamelike macrophages might migrate toward the shear zone where they differentiate into Iaantigen-presenting flamelike cells (ACPase reactive flamelike cells). It should be noted that the toothrelated zone of the periodontal ligament is an avascular zone (BEERTSEN et al, 1974) where only dendritic cells (flamelike cells without ACPase activity) are located. It is assumed, therefore, that the dendritic cells in the periodontal ligament of rat incisors might be the derivative of the flamelike macrophages that obtained Ia antigens instead of a loss of ACPase by undefined mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Histochemical and Immunohistochemical Demonstration of Macrophages and Dendritic Cells in the Lingual Periodontal Ligament of Rat Incisors.

Kawahara

Takano

Sato

et al. 1992

Archives of Histology and Cytology

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Summary. The distribution of macrophages in the lingual periodontal ligament of rat incisors was surveyed by histochemical and immunohistochemical methods. Numerous macrophages showing intense ACPase reactions were located primarily in the shear zone of the periodontal ligament. Immunostaining with an ED1-monoclonal antibody that recognizes various subpopulations of macrophages revealed plentiful positive cells showing flamelike profiles throughout the periodontal ligament, in addition to regular macrophages associated with sinusoidal blood vessels. A similar distribution of flamelike cells expressing Ia antigens was demonstrable with immunostaining using an OX6-monoclonal antibody. A consecutive staining of sections for ACPase histochemistry followed by immunoreactions for Ia antigens revealed the presence of two types of the flamelike cells in the periodontal ligament: one with and the other without distinct ACPase activity, corresponding to the macrophage and the dendritic cell, respectively. Either type of flamelike cells was located in the bone-related and shear zones, whereas only dendritic cells without ACPase activity were restricted to the tooth-related zone. OX6-immunonegative cells showing ACPase reactions were also found in the periphery of the sinusoidal blood vessels.Our data are the first to demonstrate the abundance of macrophages and dendritic cells expressing Ia antigens throughout the lingual periodontal ligament of rat incisors. In addition to regular macrophages, an exclusive localization of macrophages with flamelike extensions has been demonstrated in the bone-related and shear zones of the ligament. The region-specific arrangement of macrophages and dendritic cells with various histochemical and immunological features suggests that the periodontal ligament of rat incisor is a useful model for analyzing the process of differentiation of antigen-presenting cells.

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“…Namely, collagen fi brils taken up by phagocytosis into the fibroblasts or macrophages from the extracellular matrix were accompa nied by rapid collagen remodeling on the uterus [Parakkal. 1969] and periodontal ligament [Beertsen et al. 1974;Garant.…”

Section: Introductionmentioning

confidence: 99%

Morphological Evidence of the Formation of Intracellular Collagen Fibrils in the Embryonic Mouse Molar Odontoblasts Induced by Colchicine Administration

Ishizeki¹,

Sakakura²,

Nawa³

et al. 1989

Cells Tissues Organs

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The effects of colchicine on collagen formation were examined ultrastructurally using secretory odontoblasts in mouse molar tooth germs isografted to the spleen for 1 week. Colchicine in concentrations of 0.025 or 0.05 mg/0.1 ml was injected intravenously 12–24 h prior to harvesting. Colchicine induced the disruption of the Golgi apparatus and caused the accumulation of various types of Golgi-associated vacuoles containing collagenous fibrillar structures. Many vacuoles containing fine particles, nonstriated parallel filaments, banding patterns with a periodicity of approximately 63-nm intervals, and occasionally segment-long-spacing-like assemblies were aggregated in the cytoplasm during the experimental period. These morphological changes in vacuole contents may reflect the initial steps for polymerization of the intracellular collagen fibrils.The majority of the aggregated vacuoles were degraded by fusion with lysosomes but banded filamentous material in some vacuoles appeared to polymerize into the collagen fibrils with native structures. These results suggested that in unsecreted vacuoles accumulated in the odontoblasts as a result of colchicine administration the polymerization of collagen fibrils with native structures can occur.

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Fine structure of fibroblasts in the periodontal ligament of the rat incisor and their possible role in tooth eruption

Cited by 146 publications

References 35 publications

Immunohistochemical localization of periostin in tooth and its surrounding tissues in mouse mandibles during development

Immunohistochemical localization of periostin in tooth and its surrounding tissues in mouse mandibles during development

Histochemical and Immunohistochemical Demonstration of Macrophages and Dendritic Cells in the Lingual Periodontal Ligament of Rat Incisors.

Morphological Evidence of the Formation of Intracellular Collagen Fibrils in the Embryonic Mouse Molar Odontoblasts Induced by Colchicine Administration

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