Fine structural detection of calcium ions by photoconversion

Poletto, Valentina; Galimberti, V.; Guerra, Germano; Rosti, Vittorio; Moccia, Francesco; Biggiogera, Marco

doi:10.4081/ejh.2016.2695

Cited by 8 publications

(7 citation statements)

References 23 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…117-120 Applications of electron microscopy analyses were used for tridimensional reconstruction of apoptotic nuclei 121 and for detecting metal contaminants. 122-124 Diaminobenzidine photo-oxidation by fluorescent probes was used for detecting at electron microscopy calcium ions, 125 for visualizing endocytotic pathways 126 and for tracking nanoparticles inside the cells. 127,128 These last articles demonstrate that new varied applications in nanomedicine may be envisaged for ultrastructural histochemistry.…”

Section: Methods and Techniquesmentioning

confidence: 99%

Is there still room for novelty, in histochemical papers?

Pellicciari

2016

Eur J Histochem

View full text Add to dashboard Cite

Histochemistry continues to be widely applied in biomedical research, being nowadays mostly addressed to detect and locate single molecules or molecular complexes inside cells and tissues, and to relate structural organization and function at the high resolution of the more advanced microscopical techniques. In the attempt to see whether histochemical novelties may be found in the recent literature, the articles published in the European Journal of Histochemistry in the period 2014-2016 have been reviewed. In the majority of the published papers, standardized methods have been preferred by scientists to make their results reliably comparable with the data in the literature, but several papers (approximately one fourth of the published articles) described novel histochemical methods and procedures. It is worth noting that there is a growing interest for minimally-invasive in vivo techniques (magnetic resonance imaging, autofluorescence spectroscopy), which may parallel conventional histochemical analyses to acquire evidence not only on the morphological features of living organs and tissues, but also on their functional, biophysical and molecular characteristics. Thanks to this unceasing methodological refinement, histochemistry will continue to provide innovative applications in the biomedical field.

show abstract

Section: Methods and Techniquesmentioning

confidence: 99%

Is there still room for novelty, in histochemical papers?

Pellicciari

2016

Eur J Histochem

View full text Add to dashboard Cite

show abstract

“…Due to the physical requirements of loading dyes, most of the characterization of synthetic calcium dyes has occurred in a variety of cell cultures or tissue slices, from mice [23,32,41], rat [42,43], Drosophila [44,45], Xenopus [46,47], and human lines [48][49][50]. In monitoring Ca 2+ dynamics during neuronal activity in brain tissue slices, tissues undergoing calcium AM staining exhibited comparable functional ranges of the dye, intracellular [Ca 2+ ], and viability of neurons even after incubation for >24 h within a carefully controlled recovery chamber [51].…”

Section: Intracellular Delivery Of Dyesmentioning

confidence: 99%

“…Poletto et al visualized Ca 2+ distribution in the ER of HeLa cells by photoconverting fluorochromes using diaminobenzidine photo-oxidation. This efficiently localizes Ca 2+ at the electron microscopy level, thus combining the specificity of available fluorophores such as Mag-Fura-2 to the high spatial resolution of transmission electron microscopy [48].…”

Section: Targeting For Subcellular Localizationmentioning

confidence: 99%

Optimizing Calcium Detection Methods in Animal Systems: A Sandbox for Synthetic Biology

Saha

2021

Biomolecules

View full text Add to dashboard Cite

Since the 1970s, the emergence and expansion of novel methods for calcium ion (Ca2+) detection have found diverse applications in vitro and in vivo across a series of model animal systems. Matched with advances in fluorescence imaging techniques, the improvements in the functional range and stability of various calcium indicators have significantly enhanced more accurate study of intracellular Ca2+ dynamics and its effects on cell signaling, growth, differentiation, and regulation. Nonetheless, the current limitations broadly presented by organic calcium dyes, genetically encoded calcium indicators, and calcium-responsive nanoparticles suggest a potential path toward more rapid optimization by taking advantage of a synthetic biology approach. This engineering-oriented discipline applies principles of modularity and standardization to redesign and interrogate endogenous biological systems. This review will elucidate how novel synthetic biology technologies constructed for eukaryotic systems can offer a promising toolkit for interfacing with calcium signaling and overcoming barriers in order to accelerate the process of Ca2+ detection optimization.

show abstract

“…A cytochemical approach using osmium ammine staining allowed to describe the DNA organization in the chromatin structure of mammalian nuclei. 86 In several papers, diaminobenzidine photo-oxidation was applied to visualize fluorescent probes at TEM: by this approach, calcium ions were detected and located in the endoplasmic reticulum after staining with Mag-Fura 2 dye, 87 the uptake and intracellular fate of different fluorescently-labelled nanoparticles was monitored, 88 , 89 and the different subcellular compartments involved in the endocytosis routes were precisely described after labelling the plasma membrane with the fluorescent dye, PKH26. 90 The possibility to combine diaminobenzidine photo-oxidation and gold immunolabelling was also demonstrated.…”

Section: May Electron Microscopy Still Make a Relevant Contribution Tmentioning

confidence: 99%

Ultrastructural histochemistry in biomedical research: Alive and kicking

Malatesta

2018

Eur J Histochem

View full text Add to dashboard Cite

The high-resolution images provided by the electron microscopy has constituted a limitless source of information in any research field of life and materials science since the early Thirties of the last century. Browsing the scientific literature, electron microscopy was especially popular from the 1970’s to 80’s, whereas during the 90’s, with the advent of innovative molecular techniques, electron microscopy seemed to be downgraded to a subordinate role, as a merely descriptive technique. Ultra -structural histochemistry was crucial to promote the Renaissance of electron microscopy, when it became evident that a precise localization of molecules in the biological environment was necessary to fully understand their functional role. Nowadays, electron microscopy is still irreplaceable for ultrastructural morphology in basic and applied biomedical research, while the application of correlative light and electron microscopy and of refined ultrastructural histochemical techniques gives electron microscopy a central role in functional cell and tissue biology, as a really unique tool for high-resolution molecular biology in situ.

show abstract

Fine structural detection of calcium ions by photoconversion

Cited by 8 publications

References 23 publications

Is there still room for novelty, in histochemical papers?

Is there still room for novelty, in histochemical papers?

Optimizing Calcium Detection Methods in Animal Systems: A Sandbox for Synthetic Biology

Ultrastructural histochemistry in biomedical research: Alive and kicking

Contact Info

Product

Resources

About