Fine Structural Alterations of Interphase Nuclei of Lymphocytes Stimulated to Growth Activity in Vitro

Tokuyasu, K. T.; Madden, S. C.; Zeldis, L. J.

doi:10.1083/jcb.39.3.630

Cited by 101 publications

(31 citation statements)

References 42 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4) have the high nuclear :cytoplasmic ratio and indented nucleus which characterize the mammalian small lymphocyte . However, in contrast to their mammalian counterpart, the dogfish lymphocyte has relatively diffuse chromatin, resembling that seen in phytohemagglutinin-transformed human lymphocytes (17) . The expanded chromatin of the dogfish lymphocyte is probably a manifestation of the nucleic acid synthesis here described .…”

Section: Discussionmentioning

confidence: 99%

Macromolecular Synthesis in Dogfish Peripheral Blood Cells

Pederson

Gelfant

1970

The Journal of Cell Biology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Macromolecular Synthesis in Dogfish Peripheral Blood Cells

Pederson

Gelfant

1970

The Journal of Cell Biology

View full text Add to dashboard Cite

“…The leukocyte infiltration starts 30 min after PHA application (McCorkle, Olah & Glick 1980), that is, it is too rapid for lymphocyte mitosis to occur (Tokuyasu, Madden & Zeldis 1968). Alternatively, PHA could activate the few T cells present at the injection site prior to the treatment and these might in response induce inflammation.…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of the skin‐swelling response to phytohaemagglutinin

2010

View full text Add to dashboard Cite

Summary1. The phytohaemagglutinin (PHA) skin-swelling test is widely used in immunoecology and ecotoxicology to estimate cell-mediated immunity. Although often presumed, the involvement of T cells in generating an immune response to PHA in vivo remains unclear. 2. To investigate the mechanism triggering this response we have compared in zebra finch (Taeniopygia guttata) the immune responses to two PHA isolectins differing in their biological properties and one control protein.3. In Experiment I, we applied PHA-P (the commonly used L and E isolectin mixture) into one wing-web and bovine serum allbumin (BSA) into the other. The swelling response to PHA-P was significantly stronger than to BSA confirming that the reaction is governed by PHA-specific properties. 4. In Experiment II, purified PHA-L (the T-cell-stimulating isolectin) and PHA-E (the erythroagglutinating isolectin) were compared. Contrary to our expectations, PHA-E induced a significantly stronger swelling response than PHA-L. Histological analysis revealed significantly higher quantities of erythrocytes and thrombocytes in PHA-E-treated patagia. Nevertheless, there was a positive correlation between the magnitudes of these swellings. 5.In Experiment III, we tested whether the results obtained by the application of PHA-P and pure PHA-L differ. Here, we failed to find any significant difference between these two preparations in their immunostimulatory activity. Magnitudes of the PHA-L-and PHA-P-induced swellings were positively correlated. 6. To the best of our knowledge this is the first study to compare the biological activity of purified PHA fractions in vivo and also the first to show the importance of erythroagglutination in the development of an inflammatory response to PHA-P. 7. Our results indicate that the skin-swelling test using PHA-P reliably mirrors the individual general proinflammatory potential. However, the immunological background of the test is highly complex and the test results cannot be interpreted as measurements of the adaptive immunity or T-cell activity. This interpretational change importantly alters our view on the test results regarding the costs of the response or the evolutionary immunological adaptations.

show abstract

“…Since nuclear growth appears to be closely associated with the period of DNA synthesis [ 1,10,28], nuclear size profiles may be a reflection of the number of cells proliferating in vivo. For example, human peripheral blood lymphocytes had an almost eightfold increase in nuclear volume after exposure to the mitogen, Phytohemagglutinin [ 101.…”

Section: Discussionmentioning

confidence: 99%

Alterations of nuclear size profiles in AKR/J mice with spontaneous or transplanted leukemia

1983

View full text Add to dashboard Cite

Electronic sizing with a Coulter Counter was used to measure the frequency and number of cells with a large nuclear volume in tissues from AKR/J mice with spontaneous, long passaged, or first passage leukemia. The mean percentage of cells with a large nuclear volume was 1% in the blood, 4% in the spleen, 10% in the bone marrow, and 5% in the thymus of nonleukemic AKR/J mice. The percentage of cells with a large nucleus increased to 14-83% in all four tissues from AKR/J mice with spontaneous leukemia. In mice with a long passaged leukemia, a progressive increase in the percentage of cells with a large nucleus in the spleen and blood was associated with an increase in the number of cells/tissue with a large nucleus. However, in the bone marrow, an increase in percentage of cells with a large nuclear volume appeared to be the result of a decrease in the number of cells/humerus with a small nuclear volume. In the thymus, there was only a slight increase in the percentage of cells with a large nucleus. In mice with a first passage leukemia, there was a progressive increase, until death of the animals (21 +/- 1.9 days), in thymus weight and in the percentage of cells with a large nucleus. Also, the number of cells with a large nucleus increased in the spleen and bone marrow until day 12. Thereafter, there was a decrease in the total number of cells and number of cells with a large nucleus.

show abstract

Fine Structural Alterations of Interphase Nuclei of Lymphocytes Stimulated to Growth Activity in Vitro

Cited by 101 publications

References 42 publications

Macromolecular Synthesis in Dogfish Peripheral Blood Cells

Macromolecular Synthesis in Dogfish Peripheral Blood Cells

Functional analysis of the skin‐swelling response to phytohaemagglutinin

Alterations of nuclear size profiles in AKR/J mice with spontaneous or transplanted leukemia

Contact Info

Product

Resources

About