Fine specificity and idiotype expression of antiphosphorylcholine IgE and IgG antibodies

Weber, Ernst; Heusser, Christoph; Blaser, Kurt

doi:10.1002/eji.1830160808

Cited by 10 publications

(11 citation statements)

References 29 publications

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“…Both p and y3 isotypes are regulated mainly by T-independent mechanisms. In contrast, e is regu lated exclusively in a T-dependent way and formation of this isotype was not observed in old mice [7] and not with pneumococci [4], The change in isotype ex pression in the course of an anti-PC-KLH response was accompanied by a reduction in the T15-Id, which is the dominant Id in the primary response and among IgM and IgG3 antibodies in the memory re sponse [3]. Thus, the clonal origin and relationship of anti-PC antibodies change with progress of immuni zation, but not with age.…”

Section: Discussionmentioning

confidence: 95%

“…The Th 2 cells produce among other factors 11-4, which is responsible for development of IgE and IgGl. Anti-PC antibodies of these classes display group II characteristics in the memory re sponse [3].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the clonal origin and relationship of anti-PC antibodies change with progress of immuni zation, but not with age. Recently, 2 populations of anti-PC antibodies have been distinguished by analy sis of the fine specificity [3,4,9]. Group I antibodies, mostly T15-Id+, are directed to the PC moiety of the hapten and pneumococci.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, it was demonstrated that in a memory response anti-PC IgM, IgG3 and IgA possess another fine specificity than IgE, IgGl, IgG2a and IgG2b. El icitation of anti-PC IgE was not successful with pneu mococci and T15-Id+ IgE was not found in serum [3,4], The T 15-Id is represented by the TEPC-15 plasmocytoma.…”

Section: Introductionmentioning

confidence: 99%

“…However, BALB/c mice respond to phosphorylcholine (PC), a major antigenic determi nant of Diplococcus pneumoniae R36A, predomi nantly with antibodies bearing the T15 idiotype (T15-Id) [1], despite the fact that the repertoire at the precursor level is more diverse [2], PC presented by the natural antigen behaves as a thymus (T)-independent antigen and generates mainly IgM anti bodies. However, if coupled to a T-dependent carrier, such as keyhole-limpet hemocyanin (KLH), anti bodies of other classes, including IgE, are elicited [3,4]. Moreover, it was demonstrated that in a memory response anti-PC IgM, IgG3 and IgA possess another fine specificity than IgE, IgGl, IgG2a and IgG2b.…”

Section: Introductionmentioning

confidence: 99%

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Age-Dependent Changes of Isotype and Idiotype Expression in the Antibody Response to the Phosphorylcholine Hapten in BALB/c Mice

Gerber¹,

Ponnappan

Heusser

et al. 1989

Int Arch Allergy Immunol

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The distribution of antibodies among different isotypes in an immune response to a given antigen reflects the immunoregulatory linkage of T and B cell compartments, the genetic background of an individual and the functional state of its immune system. In addition, idiotypes are markers for the clonal origin of antibodies and their genetic relationship. Therefore, we have analyzed the isotype patterns and development of the major idiotype (T15) in BALB/c mice of different ages, immunized with a T-cell-dependent phosphorylcholine conjugate. The response was dominated by µ, γ3 and γ1 isotypes. The proportion of these antibodies changed in the progress of immunization but not with age in the primary response. An age-dependent change of the isotype distribution was observed only in the memory response. The T15 idiotype was dominantly expressed in the primary response and decreased in the memory response by 80–95% independently of the age of the mice. The results demonstrate that 2 populations of anti-phosphorylcholine antibodies which both prefer particular isotypes are expressed according to the functional state of the T and B cell compartments with age.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Age-Dependent Changes of Isotype and Idiotype Expression in the Antibody Response to the Phosphorylcholine Hapten in BALB/c Mice

Gerber¹,

Ponnappan

Heusser

et al. 1989

Int Arch Allergy Immunol

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Suppression of the anti‐hapten IgE antibody response with hapten‐modified spleen cells

Weber

Blaser

1986

Eur J Immunol

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Spleen cells of BALB/c mice were chemically modified with phosphorylcholine or benzylpenicilloyl hapten. The i.v. administration of such cells into syngeneic animals suppressed the formation of specific IgE antibodies against the respective hapten. The IgE antibody response against ovalbumin, which was used as an immunogenic carrier for the haptens, was not affected and the anti-hapten IgG or IgG1 response remained at the levels of the controls. The suppression could be transferred into X-irradiated mice by T cells from tolerized animals. Moreover, it was demonstrated that not only the induction of IgE, but also an established anti-hapten IgE antibody response is accessible to suppression by treatment with hapten-modified spleen cells from syngeneic animals. The results indicate that the i.v. administration of antigen coupled to syngeneic spleen cells induces T cells which suppress the formation of specific IgE antibodies in the primary and the secondary response without significantly affecting the formation of IgG antibodies.

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Fine specificity and VJ usage of light chains in antibodies to the phosphorylcholine hapten

Lötscher¹,

Heusser

Amstutz

et al. 1993

Eur J Immunol

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In the memory response to the phosphorylcholine hapten (PC) two major groups of anti-PC antibodies with different fine specificities are elicited. Group I antibodies are mainly PC specific, whereas Group II antibodies are comprised of two specificities directed against the phenyl-PC and the phenyl moiety of the PC hapten. The VL gene usage of 17 monoclonal memory anti-PC antibodies were investigated by Southern blot analysis and nucleotide sequencing. Six out of eight Group I memory PC-specific antibodies used the same VK22-JK5 rearrangement as the major T15 primary response idiotype. One expressed a mutated JK1 and one employed another VK22 gene family member. A shift in specificity from PC (Group I) towards phenyl-PC (Group II) was accompanied with the usage of either VK1C-JK1 or VK1A-JK5 rearrangements. The phenyl-specific Group II antibodies expressed the V lambda 1-J lambda 1 L chain rearrangement in combination with VH M141 expressing H chains. In this specific segment of Group II antibodies most mutations were found. Thus four different VL genes were found to contribute to the fine specificity of memory response antibodies to the PC hapten in a clear structure-function relationship. The diversified fine specificity in the memory response derives mainly from the usage of different L chains with particular VJ rearrangements in combination with VH of the dominant initial response clonotype and is not primarily due to mutational events.

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Fine specificity and idiotype expression of antiphosphorylcholine IgE and IgG antibodies

Cited by 10 publications

References 29 publications

Age-Dependent Changes of Isotype and Idiotype Expression in the Antibody Response to the Phosphorylcholine Hapten in BALB/c Mice

Age-Dependent Changes of Isotype and Idiotype Expression in the Antibody Response to the Phosphorylcholine Hapten in BALB/c Mice

Suppression of the anti‐hapten IgE antibody response with hapten‐modified spleen cells

Fine specificity and VJ usage of light chains in antibodies to the phosphorylcholine hapten

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