Fine-scale landscape genomics helps explain the slow spread of<i>Wolbachia</i>through the<i>Aedes aegypti</i>population in Cairns, Australia

Schmidt, Thomas L; Filipović, Igor; Hoffmann, Ary A.; Rašić, Gordana

doi:10.1101/103598

Cited by 8 publications

(25 citation statements)

References 85 publications

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“…Wolbachia displayed patchy and incomplete spread through Ae. aegypti populations in two suburbs of Cairns, Australia (25); however it displayed rapid wave-like spread through Drosophila simulans populations in California (46). The difference is thought to be due to shorter dispersal and a higher fitness cost in Ae.…”

Section: Conclusion and Future Outlooksmentioning

confidence: 99%

Can CRISPR-based gene drive be confined in the wild? A question for molecular and population biology

Marshall

Akbari

2017

Preprint

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Abstract:The recent discovery of CRISPR and its application as a gene editing tool has enabled a range of gene drive systems to be engineered with much greater ease. In order for the benefits of this technology to be realized, drive systems must be developed that are capable of both spreading into populations to achieve their desired impact, and being recalled in the event of unwanted consequences or public disfavor. We review the performance of three broad categories of drive systems at achieving these goals -threshold-dependent drives, homing-based drive and remediation systems, and temporally self-limiting systems such as daisy-chain drives.Introduction:

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Section: Conclusion and Future Outlooksmentioning

confidence: 99%

Can CRISPR-based gene drive be confined in the wild? A question for molecular and population biology

Marshall

Akbari

2017

Preprint

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“…albopictus samples within countries [17,18] and cities [26], structure at these scales is regularly observed in Ae. aegypti, the primary Dengue vector [27][28][29][30][31]. This seems incongruous given that the estimates of the flight range potential in Ae.…”

Section: Introductionmentioning

confidence: 99%

“…Analyses of Ae. aegypti at genome-wide single nucleotide polymorphisms (SNPs) discovered through double-digest restriction-site associated DNA sequencing (ddRADseq [39]) have provided a wealth of new ecological information at fine scales [29-31, 40, 41], including direct evidence of passive dispersal and spatial genetic structure at distances < 4km [31].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide SNPs Reveal the Drivers of Gene Flow In An Urban Population of the Asian Tiger Mosquito,Aedes albopictus

Schmidt

Rašić

Zhang³

et al. 2017

Preprint

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Aedes albopictus is a highly invasive disease vector with an expanding worldwide distribution. Genetic assays using low to medium resolution markers have found little evidence of spatial genetic structure even at broad geographic scales, suggesting frequent passive movement along human transportation networks. Here we analysed genetic structure of Ae. albopictus collected from 12 sample sites in Guangzhou, China, using thousands of genome-wide single nucleotide polymorphisms (SNPs). We found evidence for passive gene flow, with distance from shipping terminals being the strongest predictor of genetic distance among mosquitoes. As further evidence of passive dispersal, we found multiple pairs of full-siblings distributed between two sample sites 3.7 km apart. After accounting for geographical variability, we also found evidence for isolation by distance, previously undetectable in Ae. albopictus. These findings demonstrate how large SNP datasets and spatially-explicit hypothesis testing can be used to decipher processes at finer geographic scales than formerly possible. Our approach can be used to help predict new invasion pathways of Ae. albopictus and to refine strategies for vector control that involve the transformation or suppression of mosquito populations.. CC-BY-NC4.0 International license not peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint ( Author SummaryAedes albopictus, the Asian Tiger Mosquito, is a highly invasive disease vector with a growing global distribution. Designing strategies to prevent invasion and to control Ae. albopictus populations in invaded regions requires knowledge of how Ae. albopictus disperses. Studies comparing Ae. albopictus populations have found little evidence of genetic structure even between distant populations, suggesting that dispersal along human transportation networks is common. However, a more specific understanding of dispersal processes has been unavailable due to an absence of studies using high-resolution genetic markers. Here we present a study using high-resolution markers, which investigates genetic structure among 152 Ae. albopictus from Guangzhou, China. We found that human transportation networks, particularly shipping terminals, had an influence on genetic structure. We also found genetic distance was correlated with geographical distance, the first such observation in this species. This study demonstrates how high-resolution markers can be used to investigate ecological processes that may otherwise escape detection. We conclude that strategies for controlling Ae. albopictus will have to consider both passive reinvasion along human transportation networks and active reinvasion from neighbouring regions.

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“…and others[99] and Schmidt and others[100], but modified the protocol for pooled mosquitoes. DNA was extracted from four pools of 20 adult mosquito heads from each population, with two pools for each sex, using a Roche DNA Isolation Kit for Cells and Tissues (Roche, Pleasanton, CA, USA).…”

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A comprehensive assessment of inbreeding and laboratory adaptation inAedes aegyptimosquitoes

Ross

Endersby‐Harshman

Hoffmann

2017

Preprint

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Modified Aedes aegypti mosquitoes reared in laboratories are being released around the world to control wild mosquito populations and the diseases they transmit. Several efforts have failed due to poor competitiveness of the released mosquitoes. We hypothesized that colonized mosquito populations could suffer from inbreeding depression and adapt to laboratory conditions, reducing their performance in the field. We established replicate populations of Ae. aegypti mosquitoes collected from Queensland, Australia, and maintained them in the laboratory for twelve generations at different census sizes. Mosquito colonies maintained at small census sizes (≤100 individuals) suffered from inbreeding depression due to low effective population sizes which were only 25% of the census size as estimated by SNP markers. Populations that underwent full-sib mating for 9 consecutive generations had greatly reduced performance across all traits measured. We compared the established laboratory populations with their ancestral population resurrected from quiescent eggs for evidence of laboratory adaptation. The overall performance of laboratory populations maintained at a large census size (400 individuals) increased, potentially reflecting adaptation to artificial rearing conditions. However most individual traits were unaffected, and patterns of adaptation were not consistent across populations. Differences between replicate populations may indicate that founder effects and drift affect experimental outcomes. Though we find limited evidence of laboratory adaptation, mosquitoes maintained at low population sizes can clearly suffer fitness costs, compromising the success of "rear and release" strategies for arbovirus control.

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Fine-scale landscape genomics helps explain the slow spread ofWolbachiathrough theAedes aegyptipopulation in Cairns, Australia

Cited by 8 publications

References 85 publications

Can CRISPR-based gene drive be confined in the wild? A question for molecular and population biology

Can CRISPR-based gene drive be confined in the wild? A question for molecular and population biology

Genome-Wide SNPs Reveal the Drivers of Gene Flow In An Urban Population of the Asian Tiger Mosquito,Aedes albopictus

A comprehensive assessment of inbreeding and laboratory adaptation inAedes aegyptimosquitoes

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