Fine-Needle Aspiration of Thyroid Nodules: Proteomic Analysis To Identify Cancer Biomarkers

Giusti, Laura; Iacconi, Pietro; Ciregia, Federica; Giannaccini, Gino; Donatini, Gian Luca; Basolo, Fulvio; Miccoli, Paolo; Pinchera, Aldo; Lucacchini, Antonio

doi:10.1021/pr8000404

Cited by 99 publications

(88 citation statements)

References 54 publications

(114 reference statements)

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“…It has been suggested by Miyagi et al that a decreased expression of PA28 in colon cancer cells reduces HLA 1 protein expression, helping cancer cells avoid effects of the immune system (78). Giusti et al observed, through a proteomic method, that PA28 significantly increased in papillary carcinoma of the thyroid gland compared to normal tissue (79). In our study, PA28 was distinctly increased in resected colorectal carcinoma than in non-tumor mucosa.…”

Section: Tumor Stage ------------------------------------------------supporting

confidence: 55%

Proteomic analysis reveals overexpression of moesin and cytokeratin 17 proteins in colorectal carcinoma

Kim

Jung²,

Lee³

et al. 2011

Oncol Rep

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Abstract. The study of tumor biomarkers was gradually facilitated by the adoption of proteomic strategies due to less invasiveness and higher sensitivity. Colorectal cancer is one of the most commonly occurring cancers worldwide and its incidence has markedly increased in Korea. While the adoption of proteomic strategies facilitated the study of tumor biomarkers, to date, no common agreement has been derived from proteomic investigations regarding tumor markers of colorectal cancer. This study was designed to find molecules differentially expressed in colorectal cancer compared to non-tumor mucosa. Four colorectal adenocarcinoma and corresponding non-tumor tissue samples were analyzed to find previously unknown proteins via two-dimensional electrophoresis and MALDI-TOF/MS spectrometry. Western blot assays and tissue microarray (TMA) immunohistochemistry were performed to validate the identified proteins. Among the twelve up-regulated and one down-regulated proteins identified, moesin, cytokeratin (KRT) 17 and carbonic anhydrase I were validated by Western blot analysis and/or immunohistochemistry. On immunohistochemistry, both moesin and KRT17 demonstrated a tendency of increased expression as pT stage advanced. Both moesin and KRT17 were not expressed in normal colorectal epithelium. These two proteins may play a role in cancer invasion and/or metastasis in colorectal carcinoma, and could be candidate biomarkers for the diagnosis and prognosis of colorectal cancer.

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Section: Tumor Stage ------------------------------------------------supporting

confidence: 55%

Proteomic analysis reveals overexpression of moesin and cytokeratin 17 proteins in colorectal carcinoma

Kim

Jung²,

Lee³

et al. 2011

Oncol Rep

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“…In particular, studies of the thyrocyte and thyroid cancer proteomics, both ex vivo [26][27][28][29] and of cell lines [25] are in a minority compared with studies of the genome and transcriptome [30,31].…”

Section: Discussionmentioning

confidence: 99%

Differential proteomic and phenotypic behaviour of papillary and anaplastic thyroid cell lines

Musso

Cara

Albanese

et al. 2013

Journal of Proteomics

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Thyroid carcinomas account for a minority of all malignant tumours but, after those of the gonads, they represent the most common forms of endocrine cancers. They include several types, among which the papillary thyroid cancer (PTC) and the anaplastic thyroid cancer (ATC) are the best known. The two hystotypes display significant biological and clinical differences: PTC is a well differentiated form of tumour with a high incidence and a good prognosis, while the ATC is less frequent but represents one of the most aggressive endocrine tumours with morphological features of an undifferentiated type. To date, as far as we know, no conclusive studies, useful to design arrays of molecular markers, have been published illustrating the phenotypic and proteomic differences between these two tumours. The aim of this work was to perform a comparative analysis of two thyroid cancer cell lines, derived respectively from papillary (BCPAP) and anaplastic (8505C) thyroid carcinomas. The comparative analysis included cell behaviour assays and proteomic analysis by 2D-PAGE and mass spectrometry. IntroductionThe human thyroid gland is composed of a basic structural unit, the follicle, consisting of a monolayer of well polarized cells, the thyrocytes, responsible for the T3/T4 hormone secretion, and of other peripheral cells, the parafollicular C cells, responsible for the secretion of calcitonin. The presence within the follicle of stem cells, or remnants of embryonic cells, has been hypothesized as the target cells for tumour initiation. A thin extracellular matrix, which includes occasional fibroblasts and inflammatory cells, is peripheral to the follicle structure. Thyroid carcinomas account for 1-2% of all malignant tumours and, after those of the gonads, they represent the most common tumours of the endocrine system. The thyroid

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“…Changes in protein expression were identified using two-dimensional electrophoresis (2DE) and peptide mass finger printing via MALDI-TOF mass spectrometry (MS), as well as Western blot analysis. A significant statistical up-regulation of 17 protein spots including DJ-1 was observed in classical PTC and/or tall cell variant PTC with respect to controls (Giusti et al, 2008).…”

Section: Thyroid Cancermentioning

confidence: 88%

“…A comparative proteome analysis was performed by (Giusti et al, 2008) in order to examine the global changes of fine needle aspiration fluid protein patterns of two variants of malignant papillary thyroid cancer PTC (classical variant and tall cell variant) respect to the controls. Changes in protein expression were identified using two-dimensional electrophoresis (2DE) and peptide mass finger printing via MALDI-TOF mass spectrometry (MS), as well as Western blot analysis.…”

Section: Thyroid Cancermentioning

confidence: 99%

PARK7 (Parkinsonism associated deglycase)

Cognata¹,

Cavallaro²

2018

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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PARK7 (also called DJ-1 or Parkinsonism associated deglycase) is a pleiotropic protein belonging to the peptidase C56 family. It acts as positive regulator of androgen receptor-dependent transcription, redox-sensitive chaperone, sensor for oxidative stress, and apparently protects neurons against oxidative stress and cell death. Dysfunctions in PARK7 are related to autosomal recessive earlyonset Parkinson disease 7 and cancer forms. Here, we review some major data on PARK7, concerning the genetic structure, the transcription regulation, the encoded protein and functions, and its implication in human diseases.

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Fine-Needle Aspiration of Thyroid Nodules: Proteomic Analysis To Identify Cancer Biomarkers

Cited by 99 publications

References 54 publications

Proteomic analysis reveals overexpression of moesin and cytokeratin 17 proteins in colorectal carcinoma

Proteomic analysis reveals overexpression of moesin and cytokeratin 17 proteins in colorectal carcinoma

Differential proteomic and phenotypic behaviour of papillary and anaplastic thyroid cell lines

PARK7 (Parkinsonism associated deglycase)

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