Fine-needle aspiration is superior to needle core biopsy as a sample acquisition method for flow cytometric analysis in suspected hematologic neoplasms

Boyd, J. J.; Smith, George D.; Hong, Hao; Mageau, Ronald; Juskevicius, Ridas

doi:10.1002/cyto.b.21183

Cited by 16 publications

(19 citation statements)

References 9 publications

(23 reference statements)

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“…Comparing to excisional biopsy, core needle biopsy is easy to perform, less traumatic, and does not require general anesthesia. However, the limited biopsy material and poor recovery of cells from core needle biopsies make it difficult triaging specimens for flow cytometry testing . Chi et al reported their experience in using “rinsing” technique of the core biopsy specimens with RPMI media .…”

Section: Flow Cytometry Studies On Hematolymphoid Neoplasmsmentioning

confidence: 99%

Issue Highlights—September 2018 (94B5)

Wang

2018

Cytometry Part B Clinical

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Section: Flow Cytometry Studies On Hematolymphoid Neoplasmsmentioning

confidence: 99%

Issue Highlights—September 2018 (94B5)

Wang

2018

Cytometry Part B Clinical

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“…29 Although excisional lymph node biopsy is the preferred method for the workup of lymphoma, the combination of both FNA and CNB may serve as the best option when a less invasive sampling is required; FNA obtains better material for flow cytometry whereas CNB preserves tissue architecture for histologic correlation. 30…”

Section: Salivary Gland Lesionsmentioning

confidence: 99%

“…In a large meta‐analysis of CNB and FNA for the subclassification of lymphoma, the median rate of nonactionable diagnoses for CNB was approximately 15%, whereas the nonactionable diagnostic rate was approximately 25% for FNA . Although excisional lymph node biopsy is the preferred method for the workup of lymphoma, the combination of both FNA and CNB may serve as the best option when a less invasive sampling is required; FNA obtains better material for flow cytometry whereas CNB preserves tissue architecture for histologic correlation …”

Section: Introductionmentioning

confidence: 99%

Fine‐needle aspiration and core needle biopsy: An update on 2 common minimally invasive tissue sampling modalities

VanderLaan

2016

Cancer Cytopathology

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Fine-needle aspiration (FNA) and core needle biopsy (CNB) represent 2 of the most common minimally invasive tissue sampling modalities. Although similar in many ways, there are significant differences in the collection, processing, interpretation, and suitability for ancillary testing that exist between FNA and CNB. This review provides a brief overview of the strengths and weaknesses of FNA compared with CNB, as well as an update regarding the landscape of recently published studies that investigate the organ-specific comparative performance metrics of FNA and CNB. A current understanding of the benefits and limitations of FNA and CNB will help the cytopathologist and the clinician alike to select the right procedure for the right patient at the right time. Cancer Cytopathol 2016;124:862-870. © 2016 American Cancer Society.

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“…Finally, in this issue, there are two articles reporting technical innovations for the clinical laboratory. This journal is an excellent forum to share experiences with various techniques associated with clinical flow cytometry, and it is hoped that the readership will benefit from the current submissions (21,22) as well as recently published technical reports (23,24). Vallangeon and coworkers (21) studied the effects of tissue disaggregation methods on the immunophenotypic detection of diffuse large B cell lymphomas.…”

Section: Technical Innovations For the Clinical Labmentioning

confidence: 99%

Issue Highlights – September 2016

McCoy¹

2016

Cytometry Part B Clinical

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This issue of Cytometry Part B: Clinical Cytometry contains nine articles, eight of which examine topics related to hematopoietic neoplasms. NON-NEOPLASTIC T CELLS IN LYMPHOMASAND LEUKEMIAS Three of these manuscripts do not directly study neoplastic cells in patient cohorts but rather present studies of non-neoplastic T cell populations found in patients with lymphoma or leukemia. In one study, Hunt and colleagues (1) describe the use of internal non-neoplastic T cells as controls in the evaluation of T cell neoplasms. The staining patterns of the normal T cells can function as a process control, assuring that all steps of sample preparation, staining, and analysis were performed correctly. Furthermore, with standardization of staining and analysis, these internal T cell can also be used as controls for the assessment of staining intensities in presumptively abnormal T cells. Caveats to this approach included the paucity of some T cell subpopulations in all patients and instrument to instrument variability in measuring staining intensities, highlighting the need for standardization in both staining and instrument set up.In a second study Gunduz et al (2) examined peripheral blood T regulatory (Treg) cells in newly diagnosed lymphoma patients (both Hodgkin and non-Hodgkin). Increasingly, immune regulators and responses in patients with neoplasms are of interest, as these may tell if and how the abnormal cells are escaping immune surveillance. Recent examples include a study of natural killer (NK) cells and NK-like T cell populations in acute myeloid leukemia (3) and a study of memory and na€ ıve T cells in chronic lymphocytic leukemia (4). Several additional studies have examined Treg cells in hematologic neoplasms (5,6). In the current study Gunduz and coworkers look at Tregs (CD41CD25highFoxP31) in the peripheral blood of cohorts of Hodgkin lymphoma (HL) patients, non-Hodgkin lymphoma (NHL) patients, and healthy subjects and attempt to correlate the number of Tregs with a variety of patient characteristics. While a number of correlations were observed with prognostic factors in both HL and NHL patients, the only correlation observed with survival was in NHL patients where the number of Tregs inversely correlated with overall survival. These results, while preliminary, suggest that immune parameters might be feasible for use as prognostic markers, although much work remains to be done to precise how and why this can be done.In related work, Wu and associates (7) queried whether reactive T cells in lymphocyte rich classical HL, nodular HL and T cell/histiocyte rich large B cell lymphoma could provide information of diagnostic utility. This study builds upon and extends the work of Seegmiller et al in which CD7 overexpressing T cells were identified in infiltrates of classical HL (8). In the current study a variety of phenotypes (T cells, CD4, CD8, B cell, NK cells dual positive CD41CD81, CD7 bright T cells) were examined in the infiltrates of these lymphomas and attempts were made to correlate these with the diag...

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Fine-needle aspiration is superior to needle core biopsy as a sample acquisition method for flow cytometric analysis in suspected hematologic neoplasms

Cited by 16 publications

References 9 publications

Issue Highlights—September 2018 (94B5)

Issue Highlights—September 2018 (94B5)

Fine‐needle aspiration and core needle biopsy: An update on 2 common minimally invasive tissue sampling modalities

Issue Highlights – September 2016

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