Fine Needle Aspiration Cytology of Hodgkin’s Lymphoma

Jogai, Sanjay; Al-Jassar, Aisha; Dey, Pranab; Adesina, Aaron O.; Amanguno, Henney G.; Francis, Isaam M.

doi:10.1159/000326036

Cited by 11 publications

(3 citation statements)

References 15 publications

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“…CD15 and CD30 are recognized as markers for the Reed-Sternberg cells of classical HL. 13,30,31 Another application of immunocytochemistry in our study could support the cytologic interpretation of clinically suspected MCs in 100%. CD56 and TTF-1 were used in three cases of a neuroendocrine tumor.…”

Section: Discussionmentioning

confidence: 60%

Immunocytochemical typing of primary tumors on fine‐needle aspiration cytologies of lymph nodes

Onofre

Pomjanski

Buckstegge

et al. 2008

Diagnostic Cytopathology

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The aim of this study was to analyze the role of immunocytochemistry as an ancillary method on routine FNACs of enlarged lymph nodes, using different markers. In a validating cohort study all patients had confirmatory histological and/or clinical follow-up. 10 FNACs were analyzed for the differentiation of Non-Hodgkin Lymphoma (NHL) from metastatic carcinoma (MC), 30 cases to identify the sites of metastatic unknown primary tumors and 16 cases were checked to confirm clinical suspicion of a specific MC. Accuracy to differentiate NHL from MC was 100%, 92.3% to identify a primary tumor site of MC, and 100% to confirm a clinical suspicion of a specific MC. In 7 cases, the site of the primary tumor remained clinically unknown. Application of immunocytochemical markers on the same slide used for microscopic diagnosis is a useful tool in the routine assessment of FNACs of lymph nodes.

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Section: Discussionmentioning

confidence: 60%

Immunocytochemical typing of primary tumors on fine‐needle aspiration cytologies of lymph nodes

Onofre

Pomjanski

Buckstegge

et al. 2008

Diagnostic Cytopathology

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“…The second large cell population seen on cytology may be mononuclear forms of Reed‐Sternberg cells. In a recent study, an overall accuracy of 91.3% was found for the diagnosis of Hodgkin's lymphoma via fine needle aspiration in human patients 19 . Although histopathology and immunohistochemistry are necessary for a definitive diagnosis, the identification of Reed–Sternberg‐like cells in cytologic samples from lymph nodes from a cat, in addition to a clinical suspicion of neoplasia, should prompt the clinical pathologist to consider Hodgkin's‐like lymphoma as a differential diagnosis.…”

Section: Discussionmentioning

confidence: 99%

What is your diagnosis? Cervical mass in a cat

Steinberg¹,

Keating²

2008

Veterinary Clinical Pathol

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An 8-year-old female spayed domestic shorthair cat was presented for several months of weight loss, decreased appetite, and 2 bilateral, ventral cervical masses. Initial cytologic samples were interpreted as reactive lymphoid hyperplasia. Evaluation of subsequent mass aspirates revealed small numbers of large binucleated and multinucleated cells resembling Reed-Sternberg cells admixed with more numerous small and intermediate-sized lymphocytes. In histopathologic sections, the normal architecture of the lymph node was largely effaced by a slightly heterogeneous mass composed of round cells arranged in densely cellular sheets with a minor population of large (25-microm diameter) mononuclear cells and a few very large (30-40-microm diameter) binucleated or multinucleated cells interpreted as Reed-Sternberg-like cells. Immunohistochemically, the large neoplastic (Reed-Sternberg-like) cells were negative for CD18, CD3, CD20, and CD79a while the background population consisted of about 70% T cells and 30% B cells. This pattern of immunohistochemical staining along with cytologic and histopathologic findings supported a diagnosis of Hodgkin's-like lymphoma, specifically, the lymphocyte-rich subtype. Hodgkin's-like lymphoma has been reported previously in cats and should be suspected when Reed-Sternberg-like cells are observed in cytologic preparations of lymph node aspirates. Histopathology and immunohistochemistry are necessary for a definitive diagnosis.

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“…Aside from mimicking non-lymphoid malignancies, some haematolymphoid malignancies also endeavour to confuse by mimicking each other. A prime example is HL which may mimic a spectrum of NHL including ALCL, DLBCL, THRLBCL, AITL, PTCL, and primary mediastinal large B-cell lymphoma [6,8,10,72,[75][76][77]. Although accuracy rates exceeding 90% have been documented from cyto-histologic correlation series, errors in diagnosis have also been well documented [8,10,78].…”

Section: Lymphomas Mimicking Each Othermentioning

confidence: 99%

Pitfalls in Lymph Node Fine Needle Aspiration Cytology

Nga

2023

Acta Cytologica

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Background Fine needle aspiration cytology (FNAC) is an accurate, minimally invasive and cost-effective biopsy method for enlarged lymph nodes. While the role of lymph node FNAC in the diagnosis of infectious or reactive conditions and metastatic malignancy is unquestioned, differing views still exist on its role in the diagnosis of lymphoma. Nevertheless, regardless of the practice setting, pitfalls and potential for error exist, and it is incumbent upon the pathologist to be aware of these pitfalls; as this is the first line of defence against errors. Summary This discussion will focus on potential interpretational errors, specifically highlighting scenarios leading to false negative and false positive diagnosis and errors in tumour classification, with an emphasis on cytomorphology. Potential entities that may fly below the radar of the pathologist – so-called “off radar entities” are also discussed, as a reminder to consider broad differentials in cases with unusual morphologic features. Some reasons for false negative diagnoses include low grade lymphomas that mimic a mixed, polymorphous reactive lymphoid population; or aspirates with a paucity of lesional cells, either through sampling error or the intrinsic nature of the entity e.g. nodular lymphocyte predominant Hodgkin lymphoma. Some of the potential causes of false positive diagnoses that are discussed include viral-associated lymphadenopathy, Kikuchi-Fujimoto lymphadenitis or benign adnexal lesions mimicking metastatic malignancy. Errors in tumour classification covered include metastatic carcinoma, sarcoma, melanoma and lymphoma mimicking each other, and Hodgkin lymphoma and its mimics. Finally, less common entities such as follicular dendritic cell sarcoma and others are briefly mentioned, to remind us of conditions that may slip under our diagnostic radar. Key Messages A systematic review of diagnostic pitfalls and traps are elucidated here, with some tips to avoid these traps. The triple approach to the diagnostic workup is emphasised; which includes rigorous clinicopathologic correlation, attention to cytomorphology and judicious application of ancillary tests.

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Fine Needle Aspiration Cytology of Hodgkin’s Lymphoma

Cited by 11 publications

References 15 publications

Immunocytochemical typing of primary tumors on fine‐needle aspiration cytologies of lymph nodes

Immunocytochemical typing of primary tumors on fine‐needle aspiration cytologies of lymph nodes

What is your diagnosis? Cervical mass in a cat

Pitfalls in Lymph Node Fine Needle Aspiration Cytology

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