Fine Needle Aspiration Cytology Diagnosis of Cutaneous Leishmaniasis in a 6-Month-Old Child

Mallik, Mrinmay K.; Das, Dilip K.; Haji, Bahiyah E.

doi:10.1159/000328345

Cited by 10 publications

(6 citation statements)

References 5 publications

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“…There are several case reports emphasizing the role of FNAC in diagnosis of cutaneous leishmaniasis. [17][18][19] Cytology smears reveal granuloma with intracellular as well as extracellular amastigotes of leishmania. The following system of grading of parasites used for splenic punctures 20 can be used for cytology smears if required.…”

Section: Fine Needle Aspiration Cytology (Fnac)mentioning

confidence: 99%

Cutaneous leishmaniasis

Adhikari

Shah

2017

J Pathol Nep

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Leishmaniasis is considered to be zoonotic disease, caused by a protozoan parasite of the genus Leishmania, and transmitted by a bite of infected female sandfly. Primary cutaneous leishmaniasis is not common disease in Nepal, however, there were cases reported from Terai region of Nepal. The patients with cutaneous leishmaniasis present with a papule or nodule at the site of inoculation, followed by formation of crusts. Differential diagnoses of cutaneous leishmaniasis include variety of skin diseases, inflammatory like impetigo, eczema, or granulomatous like sarcoidosis, lupus vulgaris, to skin tumor like basal cell carcinoma & squamous cell carcinoma. There are various procedures and laboratory techniques used to diagnose leishmaniasis. Punch skin biopsy is widely used & popular technique to diagnose cutaneous leishmaniasis. Different drugs like sodium stibogluconate, sodium antimony gluconate, Amphotericin B and Miltefosine: are used for its treatment. No vaccines are available for prevention.

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Section: Fine Needle Aspiration Cytology (Fnac)mentioning

confidence: 99%

Cutaneous leishmaniasis

Adhikari

Shah

2017

J Pathol Nep

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“…In India studies have demonstrated amastigotes in smears taken from nodular lesions in 67–100% of cases, against 40% in biopsy materials . Although there are few studies discussing the utility of FNAC in CL, cytology has been rarely applied for confirmation of cutaneous lesions of PKDL . A recent study by Hosseinzadeh et al confirmed the advantages of FNAC over scrape smear in the diagnosis of CL .…”

Section: Discussionmentioning

confidence: 99%

“…Histology reveals the tissue architecture and associated pathological changes due to PKDL as obviously expected; however, LD bodies can be more readily demonstrated in Giemsa stained FNS smears as compared to the biopsy material. Biopsy is a more invasive procedure and due to shrinkage of the parasite after tissue processing, it may be difficult to find LD bodies in tissue sections …”

Section: Discussionmentioning

confidence: 99%

Fine‐needle sampling provides appreciable diagnostic yield in lesions of post kala azar dermal leishmaniasis: Analysis of four cases from North Eastern India

Bandyopadhyay

Majumdar

Gangopadhyay

et al. 2013

Diagnostic Cytopathology

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Post-kala-azar dermal leishmaniasis (PKDL) is a sequel of visceral leishmaniasis (VL), usually occurring 6 months to 3 years after VL. Spectrum of cutaneous lesions in PKDL can be hypopigmented macules, nodules, plaques, or erythema. It is usually diagnosed clinically, supplemented by ancillary techniques like skin smear examination, histopathology, polymerase chain reaction, and monoclonal antibody test. Literature on the role of cytology in the diagnosis of PKDL is extremely limited. Here we highlight the appreciable yield of fine-needle sampling in four cases of PKDL, which may be considered as a useful diagnostic aid.

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“…After 3 h, uninternalized parasites were removed by successive washes. Parasite’s infectivity was monitored every 48 h. The coverslips were collected and stained by rapid panoptic method 76 . Briefly, the coverslips containing adhered infected macrophages were immersed in fixative solution (fast green in methanol) for 10 s, followed by the same procedure using stain solution 1 (eosin G in phosphate buffer – red colour) and stain solution 2 (thiazine dye in phosphate buffer – blue color).…”

Section: Methodsmentioning

confidence: 99%

Growth arrested live-attenuated Leishmania infantum KHARON1 null mutants display cytokinesis defect and protective immunity in mice

Santi

Lanza

Tunes

et al. 2018

Sci Rep

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There is no safe and efficacious vaccine against human leishmaniasis available and live attenuated vaccines have been used as a prophylactic alternative against the disease. In order to obtain an attenuated Leishmania parasite for vaccine purposes, we generated L. infantum KHARON1 (KH1) null mutants (ΔLikh1). This gene was previously associated with growth defects in L. mexicana. ΔLikh1 was obtained and confirmed by PCR, qPCR and Southern blot. We also generate a KH1 complemented line with the introduction of episomal copies of KH1. Although ΔLikh1 promastigote forms exhibited a growth pattern similar to the wild-type line, they differ in morphology without affecting parasite viability. L. infantum KH1-deficient amastigotes were unable to sustain experimental infection in macrophages, forming multinucleate cells which was confirmed by in vivo attenuation phenotype. The cell cycle analysis of ΔLikh1 amastigotes showed arrested cells at G2/M phase. ΔLikh1-immunized mice presented reduced parasite burden upon challenging with virulent L. infantum, when compared to naïve mice. An effect associated with increased Li SLA-specific IgG serum levels and IL-17 production. Thus, ΔLikh1 parasites present an infective-attenuated phenotype due to a cytokinesis defect, whereas it induces immunity against visceral leishmaniasis in mouse model, being a candidate for antileishmanial vaccine purposes.

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Fine Needle Aspiration Cytology Diagnosis of Cutaneous Leishmaniasis in a 6-Month-Old Child

Cited by 10 publications

References 5 publications

Cutaneous leishmaniasis

Cutaneous leishmaniasis

Fine‐needle sampling provides appreciable diagnostic yield in lesions of post kala azar dermal leishmaniasis: Analysis of four cases from North Eastern India

Growth arrested live-attenuated Leishmania infantum KHARON1 null mutants display cytokinesis defect and protective immunity in mice

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