Fine Needle Aspiration Biopsy with Adjunct Immunohistochemistry in Intraocular Tumor Management

Faulkner-Jones, Beverly E.; Foster, William; Harbour, J. William; Smith, Morton E.; Dávila, Rosa M.

doi:10.1159/000326153

Cited by 62 publications

(36 citation statements)

References 39 publications

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“…Fine needle biopsies were performed using a 25-gauge needle on untreated uveal melanomas, as previously described. 5,10 For each FNAB, the first sample was used for cytologic examination and the second for RNA analysis. Each needle pass was directed into the same location, toward the center of the tumor.…”

Section: Preparation Of Rna Samplesmentioning

confidence: 99%

An Accurate, Clinically Feasible Multi-Gene Expression Assay for Predicting Metastasis in Uveal Melanoma

Onken

Worley

Tuscan

et al. 2010

The Journal of Molecular Diagnostics

304

275

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Uveal (ocular) melanoma is an aggressive cancer that often forms undetectable micrometastases before diagnosis of the primary tumor. These micrometastases later multiply to generate metastatic tumors that are resistant to therapy and are uniformly fatal. We have previously identified a gene expression profile derived from the primary tumor that is extremely accurate for identifying patients at high risk of metastatic disease. Development of a practical clinically feasible platform for analyzing this expression profile would benefit high-risk patients through intensified metastatic surveillance , earlier intervention for metastasis , and stratification for entry into clinical trials of adjuvant therapy. Here , we migrate the expression profile from a hybridization-based microarray platform to a robust , clinically practical , PCR-based 15-gene assay comprising 12 discriminating genes and three endogenous control genes. We analyze the technical performance of the assay in a prospective study of 609 tumor samples , including 421 samples sent from distant locations. We show that the assay can be performed accurately on fine needle aspirate biopsy samples , even when the quantity of RNA is below detectable limits. Preliminary outcome data from the prospective study affirm the prognostic accuracy of the assay. This prognostic assay provides an important addition to the armamentarium for managing patients with uveal melanoma , and it provides a proof of principle for the development of similar assays for other cancers.

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Section: Preparation Of Rna Samplesmentioning

confidence: 99%

An Accurate, Clinically Feasible Multi-Gene Expression Assay for Predicting Metastasis in Uveal Melanoma

Onken

Worley

Tuscan

et al. 2010

The Journal of Molecular Diagnostics

304

275

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“…In most of these early cases, the objective of the FNAB was to establish, confirm, or refute the clinical diagnosis of the evaluated intraocular tumor and direct baseline systemic evaluation of the patient, treatment of the primary intraocular tumor, and post-treatment follow-up (1,4,5,9,(13)(14)(15) . Over the years, various authors have described and illustrated the cytomorphological and immunocytochemical features of melanocytic uveal tumor cells obtained by FNAB (1,13,(15)(16)(17)(18)(19)(20)(21) and the relative frequencies of spindle, epithelioid, mixed and necrotic tumor cells in the obtained specimens (13,15,17) . A few groups of investigators have described the correlation between cytopathological and histopathological classification of the uveal me lanoma cells in cases treated by enucleation or transcleral tumor resection, (4,10,15,17,22) and some investigators have also reported the frequency of insufficient aspirates for cytopathological diagnosis in their series and factors associated with this result (3,8,10,(21)(22)(23)(24)(25)(26)(27) .…”

Section: Introductionmentioning

confidence: 99%

Prognostic implications of cytopathologic classification of melanocytic uveal tumors evaluated by fine-needle aspiration biopsy

Augsburger¹,

Corrêa²,

Trichopoulos³

2013

Arq. Bras. Oftalmol.

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Purpose: Determine whether cytopathologic classification of melanocytic uveal tumors evaluated by fine-needle aspiration biopsy (FNAB) is a significant prognostic factor for death from metastasis. Methods: Retrospective analysis of cases of clinically diagnosed uveal melanoma evaluated by fine-needle aspiration biopsy from 1980 to 2006. Main outcome evaluated was death from metastasis. Associations between baseline clinical variables and cytopathologic classification were evaluated using cross-tabulation. Prognostic significance of cytopathologic classification was evaluated by Ka planMeier and Cox proportional hazards analysis. Results: Of 302 studied biopsies, 260 (86.1%) yielded sufficient cells for cytopathologic classification. Eighty of the 260 patients who had a sufficient specimen have already died (P=0.021), 69 from metastatic uveal melanoma. Cell type assigned by cytopathology was strongly associated with metastasis/metastatic death in this series (P=0.0048). Multivariate analysis showed cytopathologic classification to be an independently significant prognostic factor for metastatic death (P=0.0006). None of the 42 patients whose tumor yielded insufficient aspirates (sampled in at least two sites) have developed metastasis or died of metastasis thus far. Conclusion: In this series, cytopathology of fine-needle aspiration biopsy samples obtained from uveal melanomas was strongly prognostic of death from metastasis. Insufficiently aspirates (2 or more sites sampled) proved to be prognostic of a favorable outcome (i.e., not developing metastasis). Keywords

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“…Thicker gauge improves the size of the sample compared to a thinner gauge needles. FNAB complications are localized transient vitreous haemorrhage (46%) [10], retinal detachment (3.7%) [4], only two endophthalmitis has been reported (Cohen, Faulkner-Jones) [11,12], and malignant cells seeding. There is special concern when retinoblastoma (Rb) is suspected; although there are no reports of extraocular growth of Rb, despite punctures with 25-gauge or smaller needles [13,14].…”

Section: Discussionmentioning

confidence: 99%

Current experience in intraocular fine needle aspiration biopsy in Mexican-Mestizo population

Moreno-Páramo¹,

Castro²,

Pérez-Pérez³

et al. 2017

Integr Cancer Sci Therap.

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Fine Needle Aspiration Biopsy with Adjunct Immunohistochemistry in Intraocular Tumor Management

Cited by 62 publications

References 39 publications

An Accurate, Clinically Feasible Multi-Gene Expression Assay for Predicting Metastasis in Uveal Melanoma

An Accurate, Clinically Feasible Multi-Gene Expression Assay for Predicting Metastasis in Uveal Melanoma

Prognostic implications of cytopathologic classification of melanocytic uveal tumors evaluated by fine-needle aspiration biopsy

Current experience in intraocular fine needle aspiration biopsy in Mexican-Mestizo population

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