Fine mapping of the major QTLs for biochemical variation of sulforaphane in broccoli florets using a DH population

Abstract

Glucoraphanin is a major secondary metabolite found inBrassicaceaevegetables, especially broccoli, and its degradation product sulforaphane plays an essential role in anticancer. The fine mapping of sulforaphane metabolism quantitative trait loci (QTLs) in broccoli florets is necessary for future marker-assisted selection strategies. In this study, we utilized a doubled haploid population consisting of 176 lines derived from two inbred lines (86,101 and 90,196) with significant differences in sulforaphane content, coupled with extensive genotypic and phenotypic data from two independent environments. A linkage map consisting of 438 simple sequence repeats markers was constructed, covering a length of 1168.26 cM. A total of 18 QTLs for sulforaphane metabolism in broccoli florets were detected, 10 were detected in 2017, and the other 8 were detected in 2018. The LOD values of all QTLs ranged from 3.06 to 14.47, explaining 1.74–7.03% of the biochemical variation between two years. Finally, 6 QTLs (qSF-C3-1,qSF-C3-2,qSF-C3-3,qSF-C3-5,qSF-C3-6andqSF-C7) were stably detected in more than one environment, each accounting for 4.54–7.03% of the phenotypic variation explained (PVE) and a total of 30.88–34.86% of PVE. Our study provides new insights into sulforaphane metabolism in broccoli florets and marker-assisted selection breeding inBrassica oleraceacrops.

A highly efficient genetic transformation system for broccoli and subcellular localization

“…obviously observed on the cytoplasm and nucleus under laser confocal microscopy, as shown in Figure 7. Therefore, this result indicated that the genes Bol031350, which is related to glucosinolate metabolism, and CRa, which is related to resistance to clubroot, were both expressed in the cytoplasm and nucleus, which was consistent with previous studies (Li et al, 2021a;Abdel-Ghany et al, 2005;Bai et al, 2014).…”

Glucoraphanin Accumulation via Glucoraphanin Synthesis Promotion during Broccoli Germination

“…Thirdly, the side chain is modified in a process controlled by a flavin-containing monooxygenase (FMOGS-OX), which mediates the formation of different glucosinolate structures [ 18 ]. In addition, AOP3 (2-oxoglutarate-dependent dioxygenases) can catalyze the conversion of glucoraphanin to hydroxyalkyl glucosinolate, whereas MYR (myrosinase) can catalyze the degradation of glucoraphanin to sulforaphane in all Brassicaceae [ 19 , 20 ].…”

Establishment of a stable, effective and universal genetic transformation technique in the diverse species of Brassica oleracea

“…Since then, the research on important gene mining and their regulatory mechanism of B. oleracea crops has entered a period of rapid development. A series of genetic loci or genes controlling plant growth, resistance to biotic and abiotic stresses, quality composition and fertility, etc., have been excavated ( Li et al., 2021 ; Shaw et al., 2021 ; Zhang et al., 2021 ; Han et al., 2021 ; Ren et al., 2022 ; Alemán et al., 2022 ). Therefore, the effective transformation method established in this study will greatly accelerate the functional validation and regulatory mechanism study of the target genes controlling important agronomic traits of B. oleracea crops and promote rapid gene pyramiding to develop excellent target materials.…”