Fine mapping of qBK1, a major QTL for bakanae disease resistance in rice

Lee, Sais-Beul; Kim, Namgyu; Hur, Yeon-Jae; Cho, Su-Min; Kim, Tae-Heon; Lee, Ji-youn; Cho, Jun-Hyeon; Lee, Jonghee; Song, You-Chun; Seo, Young-Su; Ko, Jong-Min; Park, Dong-Soo

doi:10.1186/s12284-019-0295-9

Cited by 27 publications

(28 citation statements)

References 21 publications

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“…Research groups in India (Fiyaz et al 2014 ), Korea (Kim et al 2014 ), and Taiwan (Chen et al 2019 ) used high-throughput inoculation methods to screen for resistant materials from 92, 500, and 231 diverse rice varieties, respectively. A total of 28 QTLs for bakanae resistance were mapped on rice chromosomes 1, 3, 4, 6, 8, 9, 10 and 11 (Volante et al 2017 ; Fiyaz et al 2016 ; Lee et al 2018 ; Cheon et al 2019 ; Ji et al 2018a ; Hur et al 2015 ; Lee et al 2019 ; Kang et al 2019 ; Yang et al 2006 ; Chen et al 2019 ). Chromosome 1, where 13 QTLs are located, appears to be particularly important.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Early Defenses in Rice against Fusarium fujikuroi

Cheng

Chen

Lai

et al. 2020

Rice

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Background Bakanae is a seedborne disease caused by Fusarium fujikuroi. Rice seedlings emerging from infected seeds can show diverse symptoms such as elongated and slender stem and leaves, pale coloring, a large leaf angle, stunted growth and even death. Little is known about rice defense mechanisms at early stages of disease development. Results This study focused on investigating early defenses against F. fujikuroi in a susceptible cultivar, Zerawchanica karatals (ZK), and a resistant cultivar, Tainung 67 (TNG67). Quantitative PCR revealed that F. fujikuroi colonizes the root and stem but not leaf tissues. Illumina sequencing was conducted to analyze the stem transcriptomes of F. fujikuroi-inoculated and mock-inoculated ZK and TNG67 plants collected at 7 days post inoculation (dpi). More differentially expressed genes (DEGs) were identified in ZK (n = 169) than TNG67 (n = 118), and gene ontology terms related to transcription factor activity and phosphorylation were specifically enriched in ZK DEGs. Among the complex phytohormone biosynthesis and signaling pathways, only DEGs involved in the jasmonic acid (JA) signaling pathway were identified. Fourteen DEGs encoding pattern-recognition receptors, transcription factors, and JA signaling pathway components were validated by performing quantitative reverse transcription PCR analysis of individual plants. Significant repression of jasmonate ZIM-domain (JAZ) genes (OsJAZ9, OsJAZ10, and OsJAZ13) at 3 dpi and 7 dpi in both cultivars, indicated the activation of JA signaling during early interactions between rice and F. fujikuroi. Differential expression was not detected for salicylic acid marker genes encoding phenylalanine ammonia-lyase 1 and non-expressor of pathogenesis-related genes 1. Moreover, while MeJA did not affect the viability of F. fujikuroi, MeJA treatment of rice seeds (prior to or after inoculation) alleviated and delayed bakanae disease development in susceptible ZK. Conclusions Different from previous transcriptome studies, which analyzed the leaves of infected plants, this study provides insights into defense-related gene expression patterns in F. fujikuroi–colonized rice stem tissues. Twelve out of the 14 selected DEGs were for the first time shown to be associated with disease resistance, and JA-mediated resistance was identified as a crucial component of rice defense against F. fujikuroi. Detailed mechanisms underlying the JA-mediated bakanae resistance and the novel defense-related DEGs are worthy of further investigation.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Early Defenses in Rice against Fusarium fujikuroi

Cheng

Chen

Lai

et al. 2020

Rice

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“…Ji et al (2017) found that QTL qFfR1 was located in a 230 kb region of rice chromosome 1 in Korean japonica variety Nampyeong, and suggested that the three QTLs qBK1, qBK1.1, and qFfR1 might indicate the same gene. Lee et al (2019) narrowed down the position of the qBK1 locus to a 35 kb region between InDel 18 and InDel 19-14, and revealed that location of qBK1 is close to those of qBK1.1, and qFfR1, does not overlapping each other. Two additional QTLs including qB1 from Chunjiang 06 (Yang et al 2006) and qBK1 WD from Wonseadaesoo (Lee et al 2018) also were also found on chromosome 1.…”

Section: Discussionmentioning

confidence: 96%

“…The inoculation and evaluation of bakanae disease were conducted using a method that described by Lee et al (Lee et al 2019). The isolate CF283 of F. fujikuroi (imperfect stage of G. fujikuroi) were obtained from National Academy Agricultural Science in Korea.…”

Section: Evaluation Of Bakanae Resistance and Statistical Analysismentioning

confidence: 99%

“…Hur et al (2015) identi ed a major QTL, qBK1, on chromosome 1 from 168 BC 6 F 4 near isogenic lines (NILS) generated by crossing the resistant indica variety Shingwang with susceptible japonica variety Ilpum. Lee et al (2019) delimited the location of qBK1 to 35 kb interval between two InDel markers, InDel 18 (23.637 Mbp) and . Fiyaz et al (2016) identi ed three QTLs (qBK1.1, qBK1.2, and qBK1.3) on chromosome 1 and one QTL (qBK3.1) on chromosome 3 from the highly resistant variety Pusa 1342.…”

Section: Introductionmentioning

confidence: 99%

“…Fiyaz et al (2016) identi ed three QTLs (qBK1.1, qBK1.2, and qBK1.3) on chromosome 1 and one QTL (qBK3.1) on chromosome 3 from the highly resistant variety Pusa 1342. Physical location of qBK1.1 is close to qBK1 (Fiyaz et al 2016;Lee et al 2019). Ji et al (2017) mapped a major QTL (qFfR1) in 22.56-24.10 Mbp region on chromosome 1 using 180 F 2:3 lines derived from a cross between a resistant Korean japonica variety Nampyeong and a susceptible Korean japonica line, DongjinAD.…”

Section: Introductionmentioning

confidence: 99%

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Mapping of a major QTL, qBK1Z, for bakanae disease resistance in rice

Lee

Kim

et al. 2020

Preprint

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Background: Bakanae disease is a fungal disease of rice (Oryza sativa L.) caused by the pathogen Gibberella fujikuroi (also known as Fusarium fujikuroi). Recently the disease incidence has increased in several Asian countries and continues to spread throughout the world. No rice varieties have been developed yet to be completely resistant to this disease. With increasing need to identify various genetic resources to impart resistance to local elite varieties, this study was carried out to identify novel quantitative trait loci (QTLs) from an indica variety Zenith. Results: We performed a QTL mapping using 180 F2:9 recombinant inbred lines (RILs) derived from a cross between the resistant variety, Zenith, and the susceptible variety, Ilpum. A primary QTL study using the genotypes and phenotypes of the RILs indicated that the locus qBK1z conferring bakanae disease resistance from the Zenith was located in a 2.8 Mb region bordered by the two SSR markers, RM1331 and RM3530 on chromosome 1. The log of odds (LOD) score of qBK1z was 13.43, accounting for 30.9% of the total phenotypic variation. A finer localization of qBK1z was delimited at an approximate 730 kb interval in the physical map between Chr01_1435908 (1.43 Mbp) and RM10116 (2.16 Mbp).Conclusion: The development of a rice variety with a higher level of resistance against bakanae disease is a major challenge in many rice growing countries. Introducing qBK1z or pyramiding with other previously identified QTLs could provide effective genetic control of bakanae disease in rice.

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Targeted deletion of three CYP51s in Fusarium fujikuroi and their different roles in determining sensitivity to 14α‐demethylase inhibitor fungicides

Chengxin¹,

Luo

Zhang³

2022

Pest Management Science

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Background Fusarium fujikuroi is the pathogenic agent of rice bakanae disease and has developed serious resistance to prochloraz, a 14α‐demethylase inhibitor (DMI). Prochloraz resistance in F. fujikuroi is caused by cooperation between FfCyp51B with Cyp51A and shows cross‐resistance only to prothioconazole but not to tebuconazole, difenoconazole, propiconazole, metconazole, hexaconazole, and triadimefon. This study aimed to analyze the functions of the three Cyp51s in F. fujikuroi, especially their role in determining sensitivity to DMIs. Results The respective deletion of FfCyp51A, Cyp51B, and Cyp51C had no obvious effect on morphology, conidium germination, or pathogenicity. The involvement of growth, growth and ergosterol biosynthesis, and conidium production and ergosterol biosynthesis was observed for FfCyp51A, Cyp51B, and Cyp51C, respectively. Compared with the sensitive isolate of F. fujikuroi, the effect on sensitivity to the tested DMIs was divided into four groups: (i) both of Cyp51A and Cyp51B positively regulate the sensitivity to prochloraz and prothioconazole; (ii) Cyp51B positively regulate the sensitivity to tebuconazole and metconazole, but negatively regulate the sensitivity to difenoconazole; (iii) Cyp51A and Cyp51B play opposite roles in the sensitivity to triadimefon. Therefore, deletion of Cyp51A in F. fujikuroi confers a higher sensitivity to triadimefon, while deletion of Cyp51B results in a triadimefon‐resistant mutant isolate; (iv) deletion of Cyp51B yielded a mutant isolate that was more resistant to propiconazole and hexaconazole. Conclusion Sophisticated interactions exist within the three Cyp51 genes to DMIs fungicides sensitivity in F. fujikuroi, and Cyp51B probably plays a more critical role than Cyp51A and Cyp51C. © 2022 Society of Chemical Industry.

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Fine mapping of qBK1, a major QTL for bakanae disease resistance in rice

Cited by 27 publications

References 21 publications

Transcriptome Analysis of Early Defenses in Rice against Fusarium fujikuroi

Transcriptome Analysis of Early Defenses in Rice against Fusarium fujikuroi

Mapping of a major QTL, qBK1Z, for bakanae disease resistance in rice

Targeted deletion of three CYP51s in Fusarium fujikuroi and their different roles in determining sensitivity to 14α‐demethylase inhibitor fungicides

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