Fine mapping of Ne1, the hybrid necrosis gene complementary to Ne2 in common wheat (Triticum aestivum L.)

Li, Na; Tan, Qiuyi; Ding, Jinhua; Pan, Xinglai; Ma, Zhengqiang

doi:10.1007/s00122-021-03860-9

Cited by 4 publications

(6 citation statements)

References 57 publications

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“…We mapped to a physical interval from 383.40 Mb to 388.01 Mb on chromosome 5B of Chinese spring RefSeq v1.0, which was consistent with the interval mapped by Li and Si (Li et al 2021;Si et al 2021a). The Ne1 intersection region of the three genetic maps contains 14 candidate genes (2 encoding initiation factor 4F subunit, 2 alpha/beta-hydrolases superfamily protein, 1 late embryogenesis abundant (LEA) hydroxyproline-rich glycoprotein and 9 serine protease HtrA).…”

Section: Discussionsupporting

confidence: 85%

Fine Mapping and Distribution Analysis of Hybrid Necrosis Genes Ne1 and Ne2 in Wheat in China

Zhang

Wang

et al. 2021

Preprint

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Hybrid necrosis of wheat is caused by two dominant complementary genes Ne1 and Ne2 present in normal phenotype parents and is regarded as a barrier to gene flow between crop species. However, the necrosis alleles still occur at high frequency in modern wheat varieties. In this study, we constructed two high-density genetic maps of Ne1 and Ne2 in winter wheat. In these cultivars, Ne1 was found to be located in a span interval of 0.50 centimorgan (cM) on chromosome 5BL delimited by markers Nwu_5B_4137 and Nwu_5B_5114, while Ne2 co-segregated with markers Lseq102 and TC67744 on 2BS. Statistical analysis confirmed that the dosage effect of Ne alleles also existed in moderate and severe hybrid necrosis systems, and the symptoms of necrosis can also be affected by the genetic background. Furthermore, we clarified the discrete distribution and proportion of the Ne1 and Ne2 in China’s major wheat regions, and concluded that introduced modern cultivars directly affect the frequencies of necrosis genes in modern Chinese cultivars (lines), especially that of Ne2. Taking investigations in spring wheat together, we proposed that hybrid necrosis alleles could positively affect breeding owing to their linked excellent genes. Additionally, based on the pedigree, we speculated that the Ne1 and Ne2 in winter wheat may directly originate from wild emmer and introduced cultivars or hexaploid triticale, respectively.

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Section: Discussionsupporting

confidence: 85%

Fine Mapping and Distribution Analysis of Hybrid Necrosis Genes Ne1 and Ne2 in Wheat in China

Zhang

Wang

et al. 2021

Preprint

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“…40 Mb to 388.01 Mb on chromosome 5B of Chinese spring RefSeq v1.0, which was consistent with the interval mapped by Li and Si(Li et al 2021;Si et al 2021a). The Ne1 intersection region of the three genetic maps contains 14 candidate genes (2 encoding initiation factor 4F subunit, 2 alpha/beta-hydrolases superfamily protein, 1 late embryogenesis abundant (LEA) hydroxyproline-rich glycoprotein and 9 serine protease HtrA).…”

supporting

confidence: 88%

“…The genetic distances from Ne1 and Ne2 to their nearest SSR markers, Xbarc74 and Xbarc55, were 2.0 centimorgan (cM) and 3.2 cM, respectively (Chu et al 2006). Recently, two high-density maps of Ne1 have been published (Li et al 2021, Si et al 2021a), in which Ne1 was delimited into an approximate 4 Mb interval; however, high-throughput molecular markers for screening the necrosis gene have not yet been developed. Although Ne2 has been cloned (Si et al2021b, Hewitt et al 2021Yan et al 2021), the mechanism of hybrid necrosis is still unclear and the application of Ne2 gene-speci c selective markers in breeding is limited.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, he demonstrated that the degree in weak necrosis system is affected by the number of dominant Ne alleles (gene dosage) (Hermsen 1963a). Recently, by genotyping and phenotypic identi cation of the F 2 population of Pan555/Zheng891, Li con rmed that incomplete dominance at the two Ne loci controlled the timing and severity of necrosis in moderate and severe hybrid necrosis systems (Li et al 2021). However, the dose-effect of hybrid necrosis genes in hybrids needs to be further demonstrated.…”

Section: Introductionmentioning

confidence: 99%

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Fine mapping and distribution analysis of hybrid necrosis genes Ne1 and Ne2 in wheat in China

Zhang

Wang

et al. 2022

Theor Appl Genet

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Hybrid necrosis of wheat is caused by two dominant complementary genes Ne1 and Ne2 present in normal phenotype parents and is regarded as a barrier to gene ow between crop species. However, the necrosis alleles still occur at high frequency in modern wheat varieties. In this study, we constructed two high-density genetic maps of Ne1 and Ne2 in winter wheat. In these cultivars, Ne1 was found to be located in a span interval of 0.50 centimorgan (cM) on chromosome 5BL delimited by markers Nwu_5B_4137 and Nwu_5B_5114, while Ne2 co-segregated with markers Lseq102 and TC67744 on 2BS. Statistical analysis con rmed that the dosage effect of Ne alleles also existed in moderate and severe hybrid necrosis systems, and the symptoms of necrosis can also be affected by the genetic background. Furthermore, we clari ed the discrete distribution and proportion of the Ne1 and Ne2 in China's major wheat regions, and concluded that introduced modern cultivars directly affect the frequencies of necrosis genes in modern Chinese cultivars (lines), especially that of Ne2. Taking investigations in spring wheat together, we proposed that hybrid necrosis alleles could positively affect breeding owing to their linked excellent genes. Additionally, based on the pedigree, we speculated that the Ne1 and Ne2 in winter wheat may directly originate from wild emmer and introduced cultivars or hexaploid triticale, respectively. Key MessageFlanking markers useful for identifying hybrid necrosis alleles were identi ed by ne mapping Ne1 and Ne2 and the distribution of the two necrosis genes was investigated in Chinese elite wheat varieties.

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“…Hybrid necrosis in plants refers to a genetic autoimmune syndrome occurring in the first or subsequent generations of interspecific or intraspecific hybrid plants (Li et al 2021 ). It is favorable for reproductive isolation but harmful to gene flow among species (Chen et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic characterization and gene mapping of hybrid necrosis in Triticum durum–Haynaldia villosa amphiploids

Liu,

Huang

et al. 2024

Theor Appl Genet

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Key message Phenotypical, physiological and genetic characterization was carried out on the hybrid necrosis gene from Haynaldia villosa, and the related gene Ne-V was mapped to chromosome arm 2VL. Abstract Introducing genetic variation from wild relatives into common wheat through wide crosses is a vital strategy for enriching genetic diversity and promoting wheat breeding. However, hybrid necrosis, a genetic autoimmunity syndrome, often occurs in the offspring of interspecific or intraspecific crosses, restricting both the selection of hybrid parents and the pyramiding of beneficial genes. To utilize the germplasms of Haynaldia villosa (2n = 2x = 14, VV), we conducted wide hybridization between durum wheat (2n = 4x = 28, AABB) and multiple H. villosa accessions to synthesize the amphiploids (2n = 6x = 42, AABBVV). This study revealed that 61.5% of amphiploids derived from the above crosses exhibited hybrid necrosis, with some amphiploids even dying before reaching maturity. However, the initiation time and severity of necrosis varied dramatically among the progenies, suggesting that there were multiple genetic loci or multiple alleles in the same genetic locus conferring to hybrid necrosis in H. villosa accessions. Genetic analysis was performed on the F2 and derived F2:3 populations, which were constructed between amphiploid STH59-1 with normal leaves and amphiploid STH59-2 with necrotic leaves. A semidominant hybrid necrosis-related gene, Ne-V, was mapped to an 11.8-cM genetic interval on the long arm of chromosome 2V, representing a novel genetic locus identified in Triticum-related species. In addition, the hybrid necrosis was correlated with enhanced H2O2 accumulation and cell death, and it was influenced by the temperature and light. Our findings provide a foundation for cloning the Ne-V gene and exploring its molecular mechanism.

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Fine mapping of Ne1, the hybrid necrosis gene complementary to Ne2 in common wheat (Triticum aestivum L.)

Cited by 4 publications

References 57 publications

Fine Mapping and Distribution Analysis of Hybrid Necrosis Genes Ne1 and Ne2 in Wheat in China

Fine Mapping and Distribution Analysis of Hybrid Necrosis Genes Ne1 and Ne2 in Wheat in China

Fine mapping and distribution analysis of hybrid necrosis genes Ne1 and Ne2 in wheat in China

Phenotypic characterization and gene mapping of hybrid necrosis in Triticum durum–Haynaldia villosa amphiploids

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