Fine Mapping of <i>ui6.1</i>, a Gametophytic Factor Controlling Pollen-Side Unilateral Incompatibility in Interspecific Solanum Hybrids

Li, Wentao; Royer, Suzanne M.; Chetelat, Roger T.

doi:10.1534/genetics.110.116343

Cited by 28 publications

(40 citation statements)

References 39 publications

(40 reference statements)

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“…DNA was extracted from the mapping population in 96-well microtiter plates as previously described (19). Marker sequences and restriction enzymes used for revealing polymorphism are listed in SI Appendix , Table S1.…”

Section: Methodsmentioning

confidence: 99%

“…This cross ensured that all pollen express functional ui6.1; because both pollen factors are required for a compatible reaction on the allotriploid tester stock, inferring the genotype of ui1.1 is only possible if pollen express ui6.1. The heterozygous ui1.1/+ plant was used as female parent in the cross because recombination frequency is normally higher in female than in male gametes of tomato (19,23,24).…”

Section: Ui11mentioning

confidence: 99%

“…The ability to reject tomato pollen is dominant in interspecific F 1 hybrids between cultivated tomato and related wild SI species (i.e., SC × SI hybrids), although pollen tube arrest occurs lower in the style of hybrids, suggesting that expression of the pistil side barrier is weakened (19). Allotriploid hybrids comprised of two genomes from S. lycopersicum (SC) and one genome from S. lycopersicoides (SI) reject tomato pollen tubes lower in the style than corresponding diploid hybrids (19).…”

mentioning

confidence: 99%

“…We previously reported that two pollen factors from S. pennellii, ui1.1 and ui6.1, are required and sufficient to overcome the UI barrier on allotriploid S. lycopersicum × S. lycopersicoides hybrids (19,20). These two factors are not sufficient for compatibility on Significance Self-incompatibility (SI) in plants prevents inbreeding by rejection of pollen from closely related individuals of the same species.…”

mentioning

confidence: 99%

“…The results provide further evidence that pollen rejection in UI involves biochemical mechanisms related to SI. diploid S. lycopersicum × S. lycopersicoides hybrids (19). The ui6.1 locus encodes a pollen specific Cullin1 (CUL1) protein (21) that functions in pollen recognition by UI and SI (8).…”

mentioning

confidence: 99%

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Unilateral incompatibility gene ui1.1 encodes an S-locus F-box protein expressed in pollen of Solanum species

Chetelat

2015

Proc. Natl. Acad. Sci. U.S.A.

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109

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Unilateral interspecific incompatibility (UI) is a postpollination, prezygotic reproductive barrier that prevents hybridization between related species when the female parent is self-incompatible (SI) and the male parent is self-compatible (SC). In tomato and related Solanum species, two genes, ui1.1 and ui6.1, are required for pollen compatibility on pistils of SI species or hybrids. We previously showed that ui6.1 encodes a Cullin1 (CUL1) protein. Here we report that ui1.1 encodes an S-locus F-box (SLF) protein. The ui1.1 gene was mapped to a 0.43-cM, 43.2-Mbp interval at the S-locus on chromosome 1, but positional cloning was hampered by low recombination frequency. We hypothesized that ui1.1 encodes an SLF protein(s) that interacts with CUL1 and Skp1 proteins to form an SCF-type (Skp1, Cullin1, F-box) ubiquitin E3 ligase complex. We identified 23 SLF genes in the S. pennellii genome, of which 19 were also represented in cultivated tomato (S. lycopersicum). Data from recombination events, expression analysis, and sequence annotation highlighted 11 S. pennellii genes as candidates. Genetic transformations demonstrated that one of these, SpSLF-23, is sufficient for ui1.1 function. A survey of cultivated and wild tomato species identified SLF-23 orthologs in each of the SI species, but not in the SC species S. lycopersicum, S. cheesmaniae, and S. galapagense, pollen of which lacks ui1.1 function. These results demonstrate that pollen compatibility in UI is mediated by protein degradation through the ubiquitinproteasome pathway, a mechanism related to that which controls pollen recognition in SI.interspecific incompatibility | self-incompatibility | Solanum lycopersicum | Solanum pennellii | S-locus F-box protein

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Section: Methodsmentioning

confidence: 99%

Section: Ui11mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Unilateral incompatibility gene ui1.1 encodes an S-locus F-box protein expressed in pollen of Solanum species

Chetelat

2015

Proc. Natl. Acad. Sci. U.S.A.

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109

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Enabling proteomic studies with RNA‐Seq: The proteome of tomato pollen as a test case

et al. 2012

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Effective proteome profiling is generally considered to depend heavily on the availability of a high-quality DNA reference database. As such, proteomics has long been taxonomically restricted, with limited inroads being made into the proteomes of "non-model" organisms. However, next generation sequencing (NGS), and particularly RNA-Seq, now allows deep coverage detection of expressed genes at low cost, which in turn potentially facilitates the matching of peptide mass spectra with cognate gene sequence. To test this, we performed a quantitative analysis of the proteomes of pollen from domesticated tomato (Solanum lycopersicum) and two wild relatives that exhibit differences in mating systems and in interspecific reproductive barriers. Using a custom tomato RNA-Seq database created through 454 pyrosequencing, more than 1200 proteins were identified, with subsets showing expression differences between genotypes or in the accumulation of the corresponding transcripts. Importantly, no major qualitative or quantitative differences were observed in the characterized proteomes when mass spectra were used to interrogate either a highly curated community database of tomato sequences generated through traditional sequencing technologies, or the RNA-Seq database. We conclude that RNA-Seq provides a cost-effective and robust platform for protein identification and will be increasingly valuable to the field of proteomics.

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Interspecific reproductive barriers in the tomato clade: opportunities to decipher mechanisms of reproductive isolation

et al. 2010

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The tomato clade within the genus Solanum has numerous advantages for mechanistic studies of reproductive isolation. Its thirteen closely related species, along with four closely allied Solanum species, provide a defined group with diverse mating systems that display complex interspecific reproductive barriers. Several kinds of pre- and postzygotic barriers have already been identified within this clade. Well-developed genetic maps, introgression lines, interspecific bridging lines, and the newly available draft genome sequence of the domesticated tomato (Solanum lycopersicum) are valuable tools for the genetic analysis of interspecific reproductive barriers. The excellent chromosome morphology of these diploid species allows detailed cytological analysis of interspecific hybrids. Transgenic methodologies, well developed in the Solanaceae, allow the functional testing of candidate reproductive barrier genes as well as live imaging of pollen rejection events through the use of fluorescently tagged proteins. Proteomic and transcriptomics approaches are also providing new insights into the molecular nature of interspecific barriers. Recent progress toward understanding reproductive isolation mechanisms using these molecular and genetic tools is assessed in this review.

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Fine Mapping of ui6.1, a Gametophytic Factor Controlling Pollen-Side Unilateral Incompatibility in Interspecific Solanum Hybrids

Cited by 28 publications

References 39 publications

Unilateral incompatibility gene ui1.1 encodes an S-locus F-box protein expressed in pollen of Solanum species

Unilateral incompatibility gene ui1.1 encodes an S-locus F-box protein expressed in pollen of Solanum species

Enabling proteomic studies with RNA‐Seq: The proteome of tomato pollen as a test case

Interspecific reproductive barriers in the tomato clade: opportunities to decipher mechanisms of reproductive isolation

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