Fine mapping of an up-curling leaf locus (BnUC1) in Brassica napus

Yang, Mao; Huang, Chen-Han; Wang, Mingming; Huang, Fan; Wan, Shubei; Wang, Yangming; He, Jianbo; Guan, Rongzhan

doi:10.1186/s12870-019-1938-0

Cited by 13 publications

(18 citation statements)

References 63 publications

(53 reference statements)

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“…PCR was performed in a 20-μL reaction solution containing 2 μL DNA, 2 μL 2 mM dNTPs, 2 μL 10× PCR buffer, 1 μL Taq, 1 μL of 2 μM forward and reverse primers and 12 μL of ddH 2 O. The PCR program was carried out according to Yang’s method with minor modifications [ 41 ]. The PCR products were separated on 6% nondenatured polyacrylamide gels and detected by silver staining [ 42 ].…”

Section: Methodsmentioning

confidence: 99%

Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.

Liu

Zhao

et al. 2020

BMC Plant Biol

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Background Chlorophyll is the most important factor enabling plants to absorb, transfer and transform light energy and plays an important role in yield formation. Brassica napus is one of the most important oil crops. Breeding Brassica napus for high light efficiency by improving photosynthetic efficiency has considerable social and economic value. In Brassica napus, there have been studies of the initial location of chlorophyll in seed embryos and pericarps, but there are few reports on the fine mapping of chlorophyll QTLs. We constructed near-isogenic lines (NIL), fine-mapped a chlorophyll locus, and evaluated the effect of this dominant locus on agronomic traits. Results The cqSPDA2 locus was mapped to an interval of 21.87–22.91 Mb on the chromosome A02 of Brassica napus using doubled haploid (DH) lines. To fine-map cqSPDA2, we built NIL and designed Indel primers covering the mapping interval. The 469 individuals in the BC3F2 population were analyzed using these indel primers. Among these indel primers, 15 could narrow the mapping interval to 188 kb between Indel3 and Indel15. Next, 16 indel primers and 19 SSR primers were designed within the new narrower mapping interval, and 5 of the primer-amplified fragments were found to be polymorphic and tightly linked to the cqSPDA2 locus in the BC4F2 population. The mapping interval was narrowed to 152 kb on A02 between SSR2 and Indel15. By gene expression analysis, we found three annotated genes in the mapping interval, including BnaA02g30260D, BnaA02g30290D and BnaA02g30310D, which may be responsible for chlorophyll synthesis. Conclusions The locus cqSPDA2, a dominant QTL for chlorophyll content in Brassica napus, was fine-mapped to a 21.89–22.04 Mb interval on A02. Three annotated genes (BnaA02g30260D, BnaA02g30290D and BnaA02g30310D) that may be responsible for chlorophyll synthesis were found.

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Section: Methodsmentioning

confidence: 99%

Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.

Liu

Zhao

et al. 2020

BMC Plant Biol

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“…PCR was performed in a 20-µL reaction solution containing 2 µL DNA, 2 µL 2 mM dNTPs, 2 µL 10 × PCR buffer, 1 µL Taq, 1 µL of 2 µM forward and reverse primers and 12 µL of ddH 2 O. The PCR program was carried out according to Yang's method with minor modi cations [36]. The PCR products were separated on 6% nondenatured polyacrylamide gels and detected by silver staining [37].…”

Section: Dna Extraction and Development Of Molecular Markersmentioning

confidence: 99%

Fine Mapping of the QTL cqSPDA2 for Chlorophyll Content in Brassica Napus L.

Liu

Zhao

et al. 2020

Preprint

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Background: Chlorophyll is the most important factor enabling plants to absorb, transfer and transform light energy and plays an important role in yield formation. Brassica napus is one of the most important oil crops. Qinghai Province is located in the Qinghai-Tibet Plateau in China. Solar radiation and its intensity are higher, and the light duration is longer. Under this light condition, conducting high light efficiency breeding of Brassica napus from the perspective of improving photosynthetic efficiency has considerable social and economic value. At present, the fine mapping of chlorophyll quantitative trait loci (QTLs) in many crops has been studied, especially in rice. In Brassica napus, there have been studies on the initial location of chlorophyll in seed embryos and pericarps, but there are few reports on the fine mapping of chlorophyll QTLs. We built near-isogenic lines, fine mapped the chlorophyll locus, and evaluated the effect of the dominant locus on agronomic traits.Results: The cqSPDA2 locus was mapped in an interval of 21.87-22.91 Mb on the A02 chromosome of Brassica napus using doubled haploid (DH) lines. To fine map cqSPDA2, we built near-isogenic lines and designed Indel primers covering the mapping interval. The 469 individuals in the BC3F2 population were analyzed using these Indel primers. Among the Indel primers, 15 narrowed the mapping interval to 5.2 cM between Indel3 and Indel15. Next, 16 Indel primers and 19 SSR primers were designed within the new narrow mapping interval, and 7 primers were polymorphic and tightly linked to the cqSPDA2 locus in the BC4F2 population. The mapping interval was narrowed to 152 kb on A02 between SSR2 and Indel15. We found three annotated genes in the mapping interval, including BnaA02g30260D, BnaA02g30290D and BnaA02g30310D, which may be responsible for chlorophyll synthesis by gene expression analysis. Conclusions: The locus cqSPDA2, the dominant QTL for chlorophyll content in Brassica napus, was fine mapped to the 21.89-22.04 Mb interval on A02. Three annotated genes (BnaA02g30260D, BnaA02g30290D and BnaA02g30310D) which may be responsible for chlorophyll synthesis were found.

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“…Polymerase chain reaction (PCR) amplifications of molecular markers were performed as described previously [54]. Total DNA extraction and linkage map construction were carried out according to previous reports [61].…”

Section: Genetic Mapping Of the Bnuc2 Locusmentioning

confidence: 99%

“…Six homozygous up-curled-leaf and six flat-leaf plants were selected randomly from the BC 5 F 3 population at the seedling stage for determination of photosynthetic efficiency. The photosynthetic characteristics were determined using a Li-Cor 6400 portable photosynthesis system (Li-Cor Inc., Lincoln, NE, USA) at 23°C as described previously [54]. All data were collected between 09:00 am and 11:00 am.…”

Section: Determination Of Chlorophyll Content and Photosynthetic Effimentioning

confidence: 99%

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Fine mapping of the BnUC2 locus related to leaf up-curling and plant semi-dwarfing in Brassica napus

et al. 2020

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Background: Studies of leaf shape development and plant stature have made important contributions to the fields of plant breeding and developmental biology. The optimization of leaf morphology and plant height to improve lodging resistance and photosynthetic efficiency, increase planting density and yield, and facilitate mechanized harvesting is a desirable goal in Brassica napus. Results: Here, we investigated a B. napus germplasm resource exhibiting up-curled leaves and a semi-dwarf stature. In progeny populations derived from NJAU5737 and Zhongshuang 11 (ZS11), we found that the up-curled leaf trait was controlled by a dominant locus, BnUC2. We then fine mapped the BnUC2 locus onto an 83.19-kb interval on chromosome A05 using single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) markers. We further determined that BnUC2 was a major plant height QTL that explained approximately 70% of the phenotypic variation in two BC 5 F 3 family populations derived from NJAU5737 and ZS11. This result implies that BnUC2 was also responsible for the observed semi-dwarf stature. The fine mapping interval of BnUC2 contained five genes, two of which, BnaA05g16700D (BnaA05.IAA2) and BnaA05g16720D, were revealed by comparative sequencing to be mutated in NJAU5737. This result suggests that the candidate gene mutation (BnaA05g16700D, encoding Aux/IAA2 proteins) in the conserved Degron motif GWPPV (P63S) was responsible for the BnUC2 locus. In addition, investigation of agronomic traits in a segregated population indicated that plant height, main inflorescence length, and branching height were significantly reduced by BnUC2, whereas yield was not significantly altered. The determination of the photosynthetic efficiency showed that the BnUC2 locus was beneficial to improve the photosynthetic efficiency. Our findings may provide an effective foundation for plant type breeding in B. napus. Conclusions: Using SNP and SSR markers, a dominant locus (BnUC2) related to up-curled leaves and semi-dwarf stature in B. napus has been fine mapped onto an 83.19-kb interval of chromosome A05 containing five genes. The BnaA05.IAA2 is inferred to be the candidate gene responsible for the BnUC2 locus.

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Fine mapping of an up-curling leaf locus (BnUC1) in Brassica napus

Cited by 13 publications

References 63 publications

Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.

Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.

Fine Mapping of the QTL cqSPDA2 for Chlorophyll Content in Brassica Napus L.

Fine mapping of the BnUC2 locus related to leaf up-curling and plant semi-dwarfing in Brassica napus

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