Fine mapping and characterization of the or gene in Chinese cabbage (Brassica rapa L. ssp pekinensis)

Abstract

ABSTRACT. Orange inner leaves/heads is a qualitative trait in Chinese cabbage that is controlled by a single recessive gene. Orange head Chinese cabbage contain more carotenoids than its white head counterpart; hence, this trait is of interest to both researchers and consumers. In this study, we selected the orange head Chinese cabbage line 07A163 and the white head Chinese cabbage line Chiifu as test materials. We localized the target gene controlling the orange head trait to the A09 linkage group, with a physical distance of approximately 19.9 kb between the two markers, syau26 and syau28. This region contains six candidate genes, including Bra031539, which was predicted to encode CRTISO, a carotenoid isomerase specifically required for carotenoid biosynthesis. A comparison of the nucleic acid sequences of the two test materials revealed 88 and 7-bp deletions and 88 SNPs in the promoter region of Bra031539 in line 07A163, along with a 6-bp deletion in the first exon and early termination at the 3' end of this gene. BLAST analysis revealed that 22 amino acids were altered and 17 amino acids were lost in Bra031539 in the orange head line 07A163. We developed the BrPro1 molecular marker in the promoter region of Bra031539 that can be used for early identification of orange head materials, thereby accelerating the breeding process of orange head Chinese cabbage.

Fine mapping and candidate gene analysis of Brtri1, a gene controlling trichome development in Chinese cabbage (Brassica rapa L. ssp pekinensis)

“…One gene reported from Chinese cabbage, Bra009770, the homolog of TTG1 on chromosome A06, regulates trichome development and the seed coat color (Zhang et al, 2009). Map-based cloning is one of the most effective ways to isolate genes controlling important agronomic traits (Wei et al, 2013;Ngu et al, 2014;Zou et al, 2016). In our paper, we attempted to clone the gene controlling trichome development in Chinese cabbage using this method.…”

Developmental Stage and Shape of Embryo Determine the Efficacy of Embryo Rescue in Introgressing Orange/Yellow Color and Anthocyanin Genes of Brassica Species

“…Finally, the PCR was cooled down and kept at 4 °C. The OR mutant, responsible for orange color, was confirmed by using the OR mutant-specific primers [41].…”

RETRACTED ARTICLE: BrRNE cleaves RNA in chloroplasts, regulating retrograde signals in Brassica rapa L. ssp. pekinensis

“…The Or gene is known to cause weakly pigmented or unpigmented tissues to turn orange in cauliflower (Li et al 2001), and the Br-Or gene was mapped and shown to confer orange inner leaf color in Chinese cabbage (Feng et al 2012). Molecular markers derived from Br-or and BrCRISTO1 cause orange-colored inner leaves in Brassica rapa (Zhang et al 2013;Lee et al 2014;Zou et al 2016), and loss of BrCRISTO1 function confers orange color to the inner leaves of Chinese cabbage (Su et al 2015;Zhang et al 2015). In addition, BrPur and BrMYB2 have been mapped and shown to be tightly linked to the purple-leaf phenotype (Wang et al 2014;He et al 2020).…”