Fine mapping and candidate gene analysis of a novel PANICLE AND SPIKELET DEGENERATION gene in rice

Abstract

To better understand the molecular mechanisms that regulate inflorescence and flower architecture, we characterized the rice panicle and spikelet degeneration (psd) mutant, which exhibited small panicles with a reduced number of primary and secondary branches. The psd mutant had few spikelets per panicle, and they were deformed and could not form seeds. The psd mutant had a unique panicle organization phenotype that was not present in other aberrant panicle organization mutants. Genetic analysis revealed that the mutation was controlled by a single recessive gene. A map-based cloning strategy was employed to isolate the PSD locus. The PSD locus was initially located between RM13046 and RM5356 on chromosome 2. Recessive individuals (n = 7307) in F 2 mapping populations from two crosses between heterozygous Psdpsd plants and two indica rice varieties, IR28 and 93-11, were used to finely map the PSD locus.

FAR1-RELATED SEQUENCE (FRS) and FRS-RELATED FACTOR (FRF) Family Proteins in Arabidopsis Growth and Development

“…Beside Arabidopsis thaliana , the physiological functions of FRS family members have not been investigated in other higher plant groups. However, recent studies have suggested that putative FRS members are the candidate genes for Panicle and Spikelet Degeneration ( PSD ) gene in rice ( Zhang et al, 2015 ), and a photoperiod-dependent flowering time regulator in wheat ( Kiseleva et al, 2017 ). In addition, genomic sequencing and population genomic analysis of silver birch ( Betula pendula ) also demonstrated that a putative FRS10 member might be the candidate gene correlated with the adaptation to environment ( Salojarvi et al, 2017 ).…”

A new method based on SNP of nrDNA-ITS to identify Saccharum spontaneum and its progeny in the genus Saccharum

“…Tetra-primer ARMS PCR is rapid, simple, and economical. According to the SNP site, the tetra-primer ARMS PCR technique has been used to identify various germplasm genotypes in rice, wheat, capsicum, and other crops [ 16 – 18 ].…”

Genome-Wide Identification and Functional Characterization of FAR1-RELATED SEQUENCE (FRS) Family Members in Potato (Solanum tuberosum)

“…Prior research on Arabidopsis FHY3 and FAR1 has clarified the vital roles of FRSs in plant growth and development [54]. There are ongoing studies on the FRSs in several species, such as rice, wheat, tomato, cotton, tea, eucalyptus, silver birch (Betula pendula) and Tibetan Prunus [30][31][32]53,55,[57][58][59]. In the present study, we identified StFRS family members in potato for the first time, identified the biological pathways in which they might participate, and focused on their roles in the abiotic stress response.…”