Fine mapping a major QTL qFCC7 L for chlorophyll content in rice (Oryza sativa L.) cv. PA64s

Ye, Weijun; Hu, Shikai; Wu, Liwen; Ge, Changwei; Cui, Yongtao; Chen, Ping; Xu, Jing; Guo, Longbiao; Qian, Qian

doi:10.1007/s10725-016-0188-5

Cited by 29 publications

(13 citation statements)

References 43 publications

(43 reference statements)

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“…Chlorophyll content is a quantitative characteristic that is primarily controlled by nuclear genes and has high heritability. At present, research on chlorophyll QTLs has been performed on various crops, such as rice [8,10,12,13,15], wheat [9,11], beans [24,25], and cabbage [14], especially rice [13]. There have been studies on the location of chlorophyll in the embryo [16] and pericarp [17] of winter Brassica napus.…”

Section: Discussionmentioning

confidence: 99%

“…Chlorophyll content is a quantitative characteristic that is primarily controlled by nuclear genes and has high heritability. In recent years, researchers have analyzed QTLs of chlorophyll content in seedling leaves of different crops with different populations from different perspectives and made considerable progress, establishing a foundation for future research attempting to elucidate the molecular genetic mechanism of chlorophyll [9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Fine Mapping of the QTL cqSPDA2 for Chlorophyll Content in Brassica Napus L.

Liu

Zhao

et al. 2020

Preprint

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Background: Chlorophyll is the most important factor enabling plants to absorb, transfer and transform light energy and plays an important role in yield formation. Brassica napus is one of the most important oil crops. Qinghai Province is located in the Qinghai-Tibet Plateau in China. Solar radiation and its intensity are higher, and the light duration is longer. Under this light condition, conducting high light efficiency breeding of Brassica napus from the perspective of improving photosynthetic efficiency has considerable social and economic value. At present, the fine mapping of chlorophyll quantitative trait loci (QTLs) in many crops has been studied, especially in rice. In Brassica napus, there have been studies on the initial location of chlorophyll in seed embryos and pericarps, but there are few reports on the fine mapping of chlorophyll QTLs. We built near-isogenic lines, fine mapped the chlorophyll locus, and evaluated the effect of the dominant locus on agronomic traits.Results: The cqSPDA2 locus was mapped in an interval of 21.87-22.91 Mb on the A02 chromosome of Brassica napus using doubled haploid (DH) lines. To fine map cqSPDA2, we built near-isogenic lines and designed Indel primers covering the mapping interval. The 469 individuals in the BC3F2 population were analyzed using these Indel primers. Among the Indel primers, 15 narrowed the mapping interval to 5.2 cM between Indel3 and Indel15. Next, 16 Indel primers and 19 SSR primers were designed within the new narrow mapping interval, and 7 primers were polymorphic and tightly linked to the cqSPDA2 locus in the BC4F2 population. The mapping interval was narrowed to 152 kb on A02 between SSR2 and Indel15. We found three annotated genes in the mapping interval, including BnaA02g30260D, BnaA02g30290D and BnaA02g30310D, which may be responsible for chlorophyll synthesis by gene expression analysis. Conclusions: The locus cqSPDA2, the dominant QTL for chlorophyll content in Brassica napus, was fine mapped to the 21.89-22.04 Mb interval on A02. Three annotated genes (BnaA02g30260D, BnaA02g30290D and BnaA02g30310D) which may be responsible for chlorophyll synthesis were found.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fine Mapping of the QTL cqSPDA2 for Chlorophyll Content in Brassica Napus L.

Liu

Zhao

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Chlorophyll content is a quantitative characteristic that is primarily controlled by nuclear genes and has high heritability. At present, research on chlorophyll QTLs has been performed in different populations of various crops, such as rice [12,14,16,17], wheat [13,15], soybean [27,28], and cabbage [18], and especially rice [17]. Previous studies have examined the location of chlorophyll in the embryo [19] and pericarp [20] of winter Brassica napus., In addition, QTLs were detected under drought and salt stress conditions, and a candidate gene related to salt tolerance was predicted [21][22][23].…”

Section: Discussionmentioning

confidence: 99%

“…Overall, the molecular mechanisms of chlorophyll synthesis are very complex [11]. In recent years, researchers have analyzed QTLs for chlorophyll content in the seedling leaves of different populations of multiple crops from different perspectives, making considerable progress and establishing a foundation for future research attempting to elucidate the molecular genetic mechanisms that determine of chlorophyll content [12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.

Liu

Zhao

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Chlorophyll is the most important factor enabling plants to absorb, transfer and transform light energy and plays an important role in yield formation. Brassica napus is one of the most important oil crops. Breeding Brassica napus for high light efficiency by improving photosynthetic efficiency has considerable social and economic value. In Brassica napus , there have been studies of the initial location of chlorophyll in seed embryos and pericarps, but there are few reports on the fine mapping of chlorophyll QTLs. We constructed near-isogenic lines (NIL), fine-mapped a chlorophyll locus, and evaluated the effect of this dominant locus on agronomic traits. Results The cqSPDA2 locus was mapped to an interval of 21.87-22. 91 Mb on the chromosome A02 of Brassica napus using doubled haploid (DH) lines. To fine-map cqSPDA2 , we built NIL and designed Indel primers covering the mapping interval. The 469 individuals in the BC 3 F 2 population were analyzed using these indel primers. Among the indel primers, 15 could narrow the mapping interval to 188 kb between Indel3 and Indel15. Next, 16 indel primers and 19 SSR primers were designed within the new narrower mapping interval , and 5 of the primer-amplified fragments were found to be polymorphic and tightly linked to the cqSPDA2 locus in the BC 4 F 2 population. The mapping interval was narrowed to 152 kb on A02 between SSR2 and Indel15. By gene expression analysis, we found three annotated genes in the mapping interval, including BnaA02g30260D , BnaA02g30290D and BnaA02g30310D , which may be responsible for chlorophyll synthesis. Conclusions The locus cqSPDA2 , a dominant QTL for chlorophyll content in Brassica napus , was fine-mapped to a 21.89-22.04 Mb interval on A02 . Three annotated genes ( BnaA02g30260D , BnaA02g30290D and BnaA02g30310D ) that may be responsible for chlorophyll synthesis were found.

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“…A genetic linkage map was constructed using 208 polymorphic markers to assay leaves of the 219 lines of the Lemont ´ Yangdao4 RIL population. DNA extraction and PCR protocol followed Ye et al (2017).…”

Section: Construction Of Genetic Linkage Mapmentioning

confidence: 99%

Control of plant height by 24 alleles at 12 quantitative trait loci in rice

Zeng

Chen

et al. 2019

Crop Breed. Appl. Biotechnol.

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Plant height (PH) is controlled by quantitative trait loci (QTLs) in rice. In the present study, a recombinant inbred line population developed by crossing two rice cultivars, Lemont and Yangdao4, was grown in eight environments for QTL analysis. Multiple interval mapping detected 53 PH-QTLs, 39 of which clustered at 12 chromosome regions/putative loci. An examination of the 12 putative loci identified 24 alleles that are simultaneously involved in controlling PH. Linear regression analyses suggested that these 24 alleles function additively across the 12 loci to control PH, and plants carrying more PH-increasing alleles at the 12 loci were taller than those carrying more PH-decreasing alleles. Multiple comparison tests indicated that the effect of a single allele at the 12 loci was small and that multiple alleles must be pyramided to attain a statistically significant effect. The closest markers to the 12 loci can be used directly in marker-assisted breeding to manipulate PH.

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Fine mapping a major QTL qFCC7 L for chlorophyll content in rice (Oryza sativa L.) cv. PA64s

Abstract: Candidate gene analysis showed that there were sequence variations and expression differences in the predicted candidate gene between the two parents. These results suggest that the QTL qFCC7 L may be useful for breeding the rice varieties with higher photosynthetic rate and grain yield.

Cited by 29 publications

References 43 publications

Fine Mapping of the QTL cqSPDA2 for Chlorophyll Content in Brassica Napus L.

Fine Mapping of the QTL cqSPDA2 for Chlorophyll Content in Brassica Napus L.

Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.

Control of plant height by 24 alleles at 12 quantitative trait loci in rice

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