Fine discrimination of volatile compounds by graphene-immobilized odorant-binding proteins

Kotlowski, Caroline; Larisika, Melanie; Guérin, Patrick M.; Kleber, Christoph; Kröber, Thomas; Mastrogiacomo, Rosa; Nowak, Christoph; Pelosi, Paolo; Schütz, Stefan; Schwaighofer, Andreas; Knoll, Wolfgang

doi:10.1016/j.snb.2017.10.093

Cited by 45 publications

(42 citation statements)

References 52 publications

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“…Interestingly, this idea can also be useful to improve a natural binder. Kotlowski et al [96] produced mutants of the Italian honeybee OBP 14 with an additional disulfide bond, reducing its dynamics and leading to a higher affinity for eugenol. The team of Dr. Y. Hou [97][98][99][100] proposed another approach to improve the selectivity of artificial tongues, which could be adapted to artificial noses.…”

Section: Improvement Of the Selectivitymentioning

confidence: 99%

Section: Increase Of the Sensitivitymentioning

confidence: 99%

“…To mimic this strategy, (b) Mascini et al [103] modified ZnO nanoparticles with peptides, (c) Tanaka et al [82] immobilized peptides specific to benzaldehyde on ZnO nanowires. (d) Jaffar-Bandjee et al [108] fabricated artificial "moth antennae" (e) Gray et al [109] proposed liposomes displaying tetrameric peptides to enable cooperative binding (f) Kotlowski et al [96] used a bio-functional linker to optimize the orientation of OBPs on a graphitic surface.…”

Section: Increase Of the Sensitivitymentioning

confidence: 99%

“…In the literature, various ingenious strategies have been developed for this purpose. OBPs can be immobilized using a cysteine [37] or histidine tag [96,113] at their N-terminus so as to keep a good access of the VOCs to the binding pocket. Kotlowski et al [114], Larisika et al [114], and Zhang et al [115] proposed a solution with a bi-functional linker (Figure 7f).…”

Section: Increase Of the Sensitivitymentioning

confidence: 99%

See 3 more Smart Citations

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

Hurot

Scaramozzino

Buhot

et al. 2020

Sensors

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Artificial noses are broad-spectrum multisensors dedicated to the detection of volatile organic compounds (VOCs). Despite great recent progress, they still suffer from a lack of sensitivity and selectivity. We will review, in a systemic way, the biomimetic strategies for improving these performance criteria, including the design of sensing materials, their immobilization on the sensing surface, the sampling of VOCs, the choice of a transduction method, and the data processing. This reflection could help address new applications in domains where high-performance artificial noses are required such as public security and safety, environment, industry, or healthcare.

show abstract

Section: Improvement Of the Selectivitymentioning

confidence: 99%

Section: Increase Of the Sensitivitymentioning

confidence: 99%

Section: Increase Of the Sensitivitymentioning

confidence: 99%

Section: Increase Of the Sensitivitymentioning

confidence: 99%

See 2 more Smart Citations

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

Hurot

Scaramozzino

Buhot

et al. 2020

Sensors

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show abstract

“…In some recently assembled devices, field effect transistors have been modified by attaching OBPs to the gate electrode and measuring the variations in current produced in the presence of various ligands. A study of this type, using the OBP14 of the honey bee linked to reduced graphene oxide, successfully detected eugenol, the best volatile found for the same protein in solution fluorescent assays, but was insensitive to methyleugenol, thus exhibiting a finely tuned specificity [91,92]. High selectivity was also reported for the pigOBP1 immobilised on the gold gate electrode of a transistor, that was able to discriminate between the two enantiomers of carvone, again producing responses in agreement with data obtained by fluorescent binding assays [76].…”

Section: Transducing Strategiesmentioning

confidence: 99%

From Gas Sensors to Biomimetic Artificial Noses

2018

Self Cite

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Since the first attempts to mimic the human nose with artificial devices, a variety of sensors have been developed, ranging from simple inorganic and organic gas detectors to biosensing elements incorporating proteins of the biological olfactory system. In order to design a device able to mimic the human nose, two major issues still need to be addressed regarding the complexity of olfactory coding and the extreme sensitivity of the biological system. So far, only 50 of the approximately 300-400 functioning olfactory receptors have been de-orphanized, still a long way from breaking the human olfactory code. On the other hand, the exceptional sensitivity of the human nose is based on amplification mechanisms difficult to reproduce with electronic circuits, and perhaps novel approaches are required to address this issue. Here, we review the recent literature on chemical sensing both in biological systems and artificial devices, and try to establish the state-of-the-art towards the design of an electronic nose.

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Advances in the Production of Olfactory Receptors for Industrial Use

Cho

Park

2023

Advanced Biology

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In biological olfactory systems, olfactory receptors (ORs) can recognize and discriminate between thousands of volatile organic compounds with very high sensitivity and specificity. The superior properties of ORs have led to the development of OR‐based biosensors that have shown promising potential in many applications over the past two decades. In particular, newly designed technologies in gene synthesis, protein expression, solubilization, purification, and membrane mimetics for membrane proteins have greatly opened up the previously inaccessible industrial potential of ORs. In this review, gene design, expression and solubilization strategies, and purification and reconstitution methods available for modern industrial applications are examined, with a focus on ORs. The limitations of current OR production technology are also estimated, and future directions for further progress are suggested.

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Fine discrimination of volatile compounds by graphene-immobilized odorant-binding proteins

Cited by 45 publications

References 52 publications

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review

From Gas Sensors to Biomimetic Artificial Noses

Advances in the Production of Olfactory Receptors for Industrial Use

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