FINDUS: An Open-Source 3D Printable Liquid-Handling Workstation for Laboratory Automation in Life Sciences

Barthels, Fabian; Barthels, Ulrich; Schwickert, Marvin; Schirmeister, Tanja

doi:10.1177/2472630319877374

Cited by 38 publications

(35 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Incorporation of synthetic SrtA substrates on S. aureus : The FAM‐GSLPETGGS‐NH 2 substrate was synthesized using a 3D‐printed solid‐phase peptide synthesizer (detailed procedure in the Supporting Information). The incorporation of a synthetic substrate on the S. aureus cell wall was conducted as described previously with minor modifications .…”

Section: Methodsmentioning

confidence: 99%

Asymmetric Disulfanylbenzamides as Irreversible and Selective Inhibitors of Staphylococcus aureus Sortase A

et al. 2020

Self Cite

View full text Add to dashboard Cite

Staphylococcus aureus is one of the most frequent causes of nosocomial and community-acquired infections, with drugresistant strains being responsible for tens of thousands of deaths per year. S. aureus sortase A inhibitors are designed to interfere with virulence determinants. We have identified disulfanylbenzamides as a new class of potent inhibitors against sortase A that act by covalent modification of the active-site cysteine. A broad series of derivatives were synthesized to derive structure-activity relationships (SAR). In vitro and in silico methods allowed the experimentally observed binding affinities and selectivities to be rationalized. The most active compounds were found to have single-digit micromolar K i values and caused up to a 66 % reduction of S. aureus fibrinogen attachment at an effective inhibitor concentration of 10 μM. This new molecule class exhibited minimal cytotoxicity, low bacterial growth inhibition and impaired sortase-mediated adherence of S. aureus cells. a 100 % � 0.5 % 93 % � 5.6 % n.i. 7 a 98 % � 0.1 % 14 % � 8.9 % 18 % � 1.1 % 7 f 60 % � 0.5 % n.i. 12 % � 3.1 % 7 g 18 % � 3.9 % n.i. 17 % � 7.7 % 7 h 69 % � 1.7 % 23 % � 11 % 13 % � 8.0 % 12 a 90 % � 0.5 % n.i. 15 % � 5.7 % n.i. = no inhibition at 20 μM compound concentration. All results include the mean value and standard deviations from triplicate measurements.

show abstract

Section: Methodsmentioning

confidence: 99%

Asymmetric Disulfanylbenzamides as Irreversible and Selective Inhibitors of Staphylococcus aureus Sortase A

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Across laboratory protocols there are process steps that are common, and it these where commercially available systems are more likely to be of assistance to the individual researcher. Liquid handling, through the manipulation of pipettes and receptacles is a one example ubiquitous to a range of molecular biology protocols, with a growing number of competing vendors offering more affordable and adaptable automation options (Barthels et al, 2020).…”

Section: Protocol Variation and Usagementioning

confidence: 99%

“…The number of automation development tools, components and virtual training options available to research laboratories continues to broaden, increasing their capability to develop low-cost solutions to labour intensive processes. The advent of affordable 3D printing modalities (Jones et al, 2011;Zluhan et al, 2016;Capel et al, 2018), off the shelf actuators and readily programable microcontrollers (Mabbott, 2014;Kim et al, 2015;Wong et al, 2018) has given research laboratories the ability to produce componentry that can then be assembled, controlled and automated all for a relatively low cost (Courtemanche et al, 2018;Needs et al, 2019;Barthels et al, 2020). Open source designs and software have an important enabling effect for researchers who may not have engineering or programming expertise.…”

Section: In-house Laboratory Automationmentioning

confidence: 99%

Automation in the Life Science Research Laboratory

Holland

Davies

2020

Front. Bioeng. Biotechnol.

101

View full text Add to dashboard Cite

“…Open-source hardware has started to revolutionise wet labs in recent years. Numerous devices have been proposed in order to reduce the costs and make tools accessible [8][9][10][11][25][26][27]. Most of these works are based on a combination of 3D printing and open-source electronics, which reduces the complexity involved in building the devices.…”

Section: Open-source Hardwarementioning

confidence: 99%

“…This is unfortunate as this is the case in the early stages of biochemical research, which then lacks automation support. As an answer to this problem, there is an emerging trend in research labs to build ad hoc robots for specific experiments based on open-source 3D printing technology (e.g., [5][6][7][8][9][10]). Similarly the commercial ones, do it yourself (DIY) robots also use air displacement pipettes or syringes.…”

Section: Introductionmentioning

confidence: 99%

EvoBot: An Open-Source, Modular, Liquid Handling Robot for Scientific Experiments

2020

View full text Add to dashboard Cite

Commercial liquid handling robots are rarely appropriate when tasks change often, which is the case in the early stages of biochemical research. In order to address it, we have developed EvoBot, a liquid handling robot, which is open-source and employs a modular design. The combination of an open-source and a modular design is particularly powerful because functionality is divided into modules with simple, well-defined interfaces, hence customisation of modules is possible without detailed knowledge of the entire system. Furthermore, the modular design allows end-users to only produce and assemble the modules that are relevant for their specific application. Hence, time and money are not wasted on functionality that is not needed. Finally, modules can easily be reused. In this paper, we describe the EvoBot modular design and through scientific experiments such as basic liquid handling, nurturing of microbial fuel cells, and droplet chemotaxis experiments document how functionality is increased one module at a time with a significant amount of reuse. In addition to providing wet-labs with an extendible, open-source liquid handling robot, we also think that modularity is a key concept that is likely to be useful in other robots developed for scientific purposes.

show abstract

FINDUS: An Open-Source 3D Printable Liquid-Handling Workstation for Laboratory Automation in Life Sciences

Cited by 38 publications

References 30 publications

Asymmetric Disulfanylbenzamides as Irreversible and Selective Inhibitors of Staphylococcus aureus Sortase A

Asymmetric Disulfanylbenzamides as Irreversible and Selective Inhibitors of Staphylococcus aureus Sortase A

Automation in the Life Science Research Laboratory

EvoBot: An Open-Source, Modular, Liquid Handling Robot for Scientific Experiments

Contact Info

Product

Resources

About