Finding their way: themes in germ cell migration

Barton, Lacy J.; LeBlanc, Michelle G.; Lehmann, Ruth

doi:10.1016/j.ceb.2016.07.007

Cited by 65 publications

(72 citation statements)

References 103 publications

(100 reference statements)

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“…During the first day of embryonic development, zebrafish primordial germ cells (PGCs) migrate from the positions at which they are specified towards the region where the gonad develops (Barton et al, 2016; Paksa and Raz, 2015). The migrating zebrafish PGCs can be visualized within the developing embryo by expression of fluorescent protein fusions that label specific cellular structures (Blaser et al, 2005; Kardash et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

“…Here we used primordial germ cells (PGCs) that migrate through the developing embryo (Barton et al, 2016) as an in vivo model for investigating the cellular mechanisms contributing to protrusion formation in single cell migration. We find that the rapid expansion of the bleb-type protrusions in zebrafish PGCs depends on invaginations found around the cell circumference.…”

Section: Introductionmentioning

confidence: 99%

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Bleb Expansion in Migrating Cells Depends on Supply of Membrane from Cell Surface Invaginations

et al. 2017

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Cell migration is essential for morphogenesis, for organ formation and homeostasis, with relevance for clinical conditions. The migration of primordial germ cells (PGCs) is a useful model to study this process in the context of the developing embryo. Zebrafish PGC migration depends on the formation of cellular protrusions in form of blebs, a type of protrusion found in various cell types. Here we report on the mechanisms allowing the inflation of the membrane during bleb formation. We show that the rapid expansion of the protrusion depends on membrane invaginations that are localized preferentially at the cell front. The formation of these invaginations requires the function of Cdc42, and their unfolding allows bleb inflation and dynamic cell-shape changes performed by migrating cells. Inhibiting the formation and release of the invaginations strongly interfered with bleb formation, cell motility and with the ability of the cells to reach their target.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bleb Expansion in Migrating Cells Depends on Supply of Membrane from Cell Surface Invaginations

et al. 2017

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“…Cell homing behavior to various body locations probably follows biochemical gradient signals that guide cells to their specific destinations. Homing of some embryonic cells, such as primary germline cells, appears to be a biologic process that is highly conserved across animal species …”

Section: Natural Chimeras Exhibit Immune Tolerancementioning

confidence: 99%

“…Recall that virtually all marmoset twin chimeras and some human twin chimeras carry DZ cells in blood and extravascular tissues. Many embryonic cells, including germ cells, are capable of migration and homing to various somatic locations . Too few human twin chimeras have had tissues examined sufficiently to determine the proportions of DZ cell populations in extravascular tissues.…”

Section: Natural Human (“Macro‐“) Chimerasmentioning

confidence: 99%

A review of the biology and classification of human chimeras

Wenk¹

2018

Transfusion

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Many human chimeras remain undetected. The states of health and disease of human chimeras remain largely unknown. Of four ways to detect human chimeras, molecular typing is the most sensitive and specific. Before systematic and temporal studies can be undertaken, improved cell sampling and better analytical detection methods are necessary. Chimeras may be sought among dizygotic twins and children born after IVF procedures.

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“…The ICM differentiates into the epiblast and hypoblast, that will form the fetus and amniotic sac, respectively (Cockburn and Rossant, 2010;Rossant, 2016). The epiblast cells also give rise to primordial germ cells (PGC) (McLaren, 2000), that migrate to gonadal ridges and form the germ cells lineages (Barton et al, 2016).…”

Section: Pluripotencymentioning

confidence: 99%

Lessons learned from functional assessment of pluripotency-associated transcription factors during early embryogenesis and embryonic stem cells

et al. 2017

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Pluripotency-associated transcription factors (PATF) play significant roles during early embryogenesis and in embryonic stem (ES) cells, such as control of cell-cycle progression, modulation of cellular metabolism, and transcriptional control of differentiation-inducing factors. The review aims to describe the current understanding of how these PATFs contribute to the early embryo and the ES-cell phenotypes. By a selection of representative examples of such PATFs, their roles are described, and some interesting questions are presented concerning their activity in pluripotent cells which have yet to be addressed.Keywords: embryology; pluripotent; preimplantation development; totipotency. ResumoFatores de transcrição relacionados à pluripotência (FTRP) apresentam importantes funções durante a embriogênese inicial e em células-tronco embrionárias (CTE), como o controle da progressão do ciclo celular, modulação do metabolismo celular e controle transcricional de fatores indutores de diferenciação celular. O objetivo da revisão foi descrever o conhecimento vigente sobre como estes FTRPs contribuem para o fenótipo dos embriões e CTEs. Através da seleção de exemplos representativos destes FTRPs, suas funções são descritas e algumas perguntas interessantes são apresentadas considerando suas atividades em células pluripotentes, mas que ainda não foram devidamente respondidas.Palavras-Chave: embriologia; pluripotente; desenvolvimento pré-implantacional; totipotência.

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Finding their way: themes in germ cell migration

Cited by 65 publications

References 103 publications

Bleb Expansion in Migrating Cells Depends on Supply of Membrane from Cell Surface Invaginations

Bleb Expansion in Migrating Cells Depends on Supply of Membrane from Cell Surface Invaginations

A review of the biology and classification of human chimeras

Lessons learned from functional assessment of pluripotency-associated transcription factors during early embryogenesis and embryonic stem cells

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