Abstract:Plastic pollution is an international environmental problem.
Desire
to act is shared from the public to policymakers, yet motivation and
approaches are diverging. Public attention is directed to reducing
plastic consumption, cleaning local environments, and engaging in
citizen science initiatives. Policymakers and regulators are working
on prevention and mitigation measures, while international, regional,
and national bodies are defining monitoring recommendations. Research
activities are focused on validating… Show more
“…To further enhance our approach, several avenues could be explored, including (1) utilizing additional FC settings to capture unique features of MPs, such as using UV laser light for excitation since MPs can absorb UV light. This could provide valuable information for differentiating polymer composition.…”
Section: Discussionmentioning
confidence: 99%
“…Freshwater systems link terrestrial sources of plastics pollution and the ocean, which highlights their significance in understanding the associated transport pathways of microplastics (MPs). 1,2 MPs (<5 mm in size) enter the environment either directly (primary MPs) or are generated through the fragmentation of macroplastics due to physical and chemical weathering processes (secondary MPs). 3 Currently, there are no standardized approaches to measure MPs, and monitoring MPs in freshwater can be challenging.…”
Section: Introductionmentioning
confidence: 99%
“…Freshwater systems link terrestrial sources of plastics pollution and the ocean, which highlights their significance in understanding the associated transport pathways of microplastics (MPs). , MPs (<5 mm in size) enter the environment either directly (primary MPs) or are generated through the fragmentation of macroplastics due to physical and chemical weathering processes (secondary MPs) . Currently, there are no standardized approaches to measure MPs, and monitoring MPs in freshwater can be challenging. , Complicating their identification in complex environmental matrices further, MPs in freshwater are often suspended with other particulate matter in the water column, surrounded by an ecocorona of biomacromolecules on their surface, or incorporated with biofilms on stream and river beds. , Given that MPs are a diverse suite of contaminants, the reliable identification and quantification of MPs necessitates advanced analytical methods and robust reference libraries.…”
Microplastics (MPs) in natural waters are heterogeneously mixed with other natural particles including algal cells and suspended sediments. An easy-to-use and rapid method for directly measuring and distinguishing MPs from other naturally present colloids in the environment would expedite analytical workflows. Here, we established a database of MP scattering and fluorescence properties, either alone or in mixtures with natural particles, by stain-free flow cytometry. The resulting highdimensional data were analyzed using machine learning approaches, either unsupervised (e.g., viSNE) or supervised (e.g., random forest algorithms). We assessed our approach in identifying and quantifying model MPs of diverse sizes, morphologies, and polymer compositions in various suspensions including phototrophic microorganisms, suspended biofilms, mineral particles, and sediment. We could precisely quantify MPs in microbial phototrophs and natural sediments with high organic carbon by both machine learning models (identification accuracies over 93%), although it was not possible to distinguish between different MP sizes or polymer compositions. By testing the resulting method in environmental samples through spiking MPs into freshwater samples, we further highlight the applicability of the method to be used as a rapid screening tool for MPs. Collectively, this workflow can be easily applied to a diverse set of samples to assess the presence of MPs in a time-efficient manner.
“…To further enhance our approach, several avenues could be explored, including (1) utilizing additional FC settings to capture unique features of MPs, such as using UV laser light for excitation since MPs can absorb UV light. This could provide valuable information for differentiating polymer composition.…”
Section: Discussionmentioning
confidence: 99%
“…Freshwater systems link terrestrial sources of plastics pollution and the ocean, which highlights their significance in understanding the associated transport pathways of microplastics (MPs). 1,2 MPs (<5 mm in size) enter the environment either directly (primary MPs) or are generated through the fragmentation of macroplastics due to physical and chemical weathering processes (secondary MPs). 3 Currently, there are no standardized approaches to measure MPs, and monitoring MPs in freshwater can be challenging.…”
Section: Introductionmentioning
confidence: 99%
“…Freshwater systems link terrestrial sources of plastics pollution and the ocean, which highlights their significance in understanding the associated transport pathways of microplastics (MPs). , MPs (<5 mm in size) enter the environment either directly (primary MPs) or are generated through the fragmentation of macroplastics due to physical and chemical weathering processes (secondary MPs) . Currently, there are no standardized approaches to measure MPs, and monitoring MPs in freshwater can be challenging. , Complicating their identification in complex environmental matrices further, MPs in freshwater are often suspended with other particulate matter in the water column, surrounded by an ecocorona of biomacromolecules on their surface, or incorporated with biofilms on stream and river beds. , Given that MPs are a diverse suite of contaminants, the reliable identification and quantification of MPs necessitates advanced analytical methods and robust reference libraries.…”
Microplastics (MPs) in natural waters are heterogeneously mixed with other natural particles including algal cells and suspended sediments. An easy-to-use and rapid method for directly measuring and distinguishing MPs from other naturally present colloids in the environment would expedite analytical workflows. Here, we established a database of MP scattering and fluorescence properties, either alone or in mixtures with natural particles, by stain-free flow cytometry. The resulting highdimensional data were analyzed using machine learning approaches, either unsupervised (e.g., viSNE) or supervised (e.g., random forest algorithms). We assessed our approach in identifying and quantifying model MPs of diverse sizes, morphologies, and polymer compositions in various suspensions including phototrophic microorganisms, suspended biofilms, mineral particles, and sediment. We could precisely quantify MPs in microbial phototrophs and natural sediments with high organic carbon by both machine learning models (identification accuracies over 93%), although it was not possible to distinguish between different MP sizes or polymer compositions. By testing the resulting method in environmental samples through spiking MPs into freshwater samples, we further highlight the applicability of the method to be used as a rapid screening tool for MPs. Collectively, this workflow can be easily applied to a diverse set of samples to assess the presence of MPs in a time-efficient manner.
“…The need for the use of harmonised protocols for the sampling, processing and reporting of microplastics in environmental data has been voiced by many studies over the past years 9 , 38 . Generally, individual processes for the extraction, isolation, identification and quantification of microplastics in environmental samples are already harmonised and are presented in Fig.…”
Section: The Need For Harmonised Guidelines and Reportingmentioning
Infrastructure is often a limiting factor in microplastics research impacting the production of scientific outputs and monitoring data. International projects are therefore required to promote collaboration and development of national and regional scientific hubs. The Commonwealth Litter Programme and the Ocean Country Partnership Programme were developed to support Global South countries to take actions on plastics entering the oceans. An international laboratory network was developed to provide the infrastructure and in country capacity to conduct the collection and processing of microplastics in environmental samples. The laboratory network was also extended to include a network developed by the University of East Anglia, UK. All the laboratories were provided with similar equipment for the collection, processing and analysis of microplastics in environmental samples. Harmonised protocols and training were also provided in country during laboratory setup to ensure comparability of quality-controlled outputs between laboratories. Such large networks are needed to produce comparable baseline and monitoring assessments.
“…Investigation of this kind will unravel the relevant concentrations and the polymer type, size distribution and particle features of common MPs in freshwater bodies, providing guidance for future exposure and effect tests in laboratory. A main hindrance in this process is the need for data and methods harmonization, which is unfortunately a known issue in MPs research [ 119 , 122 ].…”
Section: Studies On Zooplankton and Microplastics In Freshwater Lakesmentioning
Lakes are essentially interlinked to humans as they provide water for drinking, agriculture, industrial and domestic purposes. The upsurge of plastic usage, its persistence, and potential detrimental effects on organisms cause impacts on the trophic food web of freshwater ecosystems; this issue, however, still needs to be explored. Zooplankton worldwide is commonly studied as an indicator of environmental risk in aquatic ecosystems for several pollutants. The aim of the review is to link the existing knowledge of microplastic pollution in zooplankton to assess the potential risks linked to these organisms which are at the first level of the lacustrine trophic web. A database search was conducted through the main databases to gather the relevant literature over the course of time. The sensitivity of zooplankton organisms is evident from laboratory studies, whereas several knowledge gaps exist in the understanding of mechanisms causing toxicity. This review also highlights insufficient data on field studies hampering the understanding of the pollution extent in lakes, as well as unclear trends on ecosystem–level cascading effects of microplastics (MPs) and mechanisms of toxicity (especially in combination with other pollutants). Therefore, this review provides insight into understanding the overlooked issues of microplastic in lake ecosystems to gain an accurate ecological risk assessment.
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