Finding a needle in a “needlestack”

Rodrigues, Melanie; Wong, Victor W.; Gurtner, Geoffrey C.

doi:10.1080/15384101.2016.1229017

Cited by 5 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The transcriptional data obtained from these tests can be subjected to hierarchical and k-means clustering to generate subsets of cells based on their intracellular transcriptional profile that can include genes for inflammation, growth factor release, extracellular matrix deposition or matrix lysis 70,104,105 . Correlation analyses can be conducted to reveal the best surface marker or surface marker combination unique to each cell subset 106,107 . Finally, the surface marker combinations can be used to isolate unique macrophage subsets by FACS.…”

Section: Identification Of Macrophage Phenotypes In Wound Healingmentioning

confidence: 99%

See 1 more Smart Citation

Black, White, and Gray: Macrophages in Skin Repair and Disease

Rodrigues

Gurtner

2017

Curr Pathobiol Rep

Self Cite

View full text Add to dashboard Cite

Purpose of Review Macrophages alter their responses during the temporal stages of wound healing. During the inflammatory phase macrophages perform phagocytosis. During neovascularization macrophages activate angiogenesis. In the proliferation phase of wound healing, macrophages deposit extracellular matrix and during wound resolution macrophages phagocytize excessive cellular components. This review addresses how these changing phenotypes affect skin repair and disease. Recent Findings Macrophages can determine the outcome of repair and can shift the normal wound healing response into fibrosis or chronic wounds. Emerging single cell technologies for the first time provide us with tools to uncover macrophage origin, heterogeneity and function. Summary Macrophages may exist as one population where all cells alter their phenotype in response to signals from the microenvironment. Alternatively, macrophages may exist as distinct subsets that can control wound outcomes. A clarified understanding will strengthen our knowledge of skin biology and aid in the development of wound healing therapies.

show abstract

Section: Identification Of Macrophage Phenotypes In Wound Healingmentioning

confidence: 99%

“…However, it is much less comprehensive compared to RNA sequencing and the primers used to probe the single cells need to be carefully selected. Despite the disadvantage, the reliability of the technique makes it is more suited for clinical diagnoses 106,107 .…”

Section: Identification Of Macrophage Phenotypes In Wound Healingmentioning

confidence: 99%