FIMBRIN1 Is Involved in Lily Pollen Tube Growth by Stabilizing the Actin Fringe

Su, Hui; Zhu, Jinsheng; Cai, Chao; Pei, Weike; Wang, Jiaojiao; Dong, Huaijian; Ren, Haiyun

doi:10.1105/tpc.112.099358

Cited by 59 publications

(57 citation statements)

References 63 publications

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“…We found that most of the CKL2 remained in the supernatant in both the absence and presence of F-actin; however, a significant amount of the actin binding protein Fim1 (Su et al, 2012) was pulled down by F-actin (Supplemental Figure 4A). …”

Section: Ckl2 Colocalizes With Actin Filaments In Cells But Does Not mentioning

confidence: 99%

“…Actin filaments are highly dynamic, undergoing rapid reorganization and turnover regulated by actin binding proteins such as ADF/cofilin, villin, profilin, fimbrin, and capping protein (Wasteneys and Galway, 2003;Hussey et al, 2006;Staiger and Blanchoin, 2006;Higaki et al, 2007;Thomas et al, 2009;Li et al, 2010;Su et al, 2012;Qu et al, 2013;Wang et al, 2015). ADF/cofilin proteins function as key regulators of actin filament dynamics and reorganization through binding to both globular and filamentous actin.…”

Section: Introductionmentioning

confidence: 99%

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CASEIN KINASE1-LIKE PROTEIN2 Regulates Actin Filament Stability and Stomatal Closure via Phosphorylation of Actin Depolymerizing Factor

et al. 2016

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The opening and closing of stomata are crucial for plant photosynthesis and transpiration. Actin filaments undergo dynamic reorganization during stomatal closure, but the underlying mechanism for this cytoskeletal reorganization remains largely unclear. In this study, we identified and characterized Arabidopsis thaliana casein kinase 1-like protein 2 (CKL2), which responds to abscisic acid (ABA) treatment and participates in ABA-and drought-induced stomatal closure. Although CKL2 does not bind to actin filaments directly and has no effect on actin assembly in vitro, it colocalizes with and stabilizes actin filaments in guard cells. Further investigation revealed that CKL2 physically interacts with and phosphorylates actin depolymerizing factor 4 (ADF4) and inhibits its activity in actin filament disassembly. During ABA-induced stomatal closure, deletion of CKL2 in Arabidopsis alters actin reorganization in stomata and renders stomatal closure less sensitive to ABA, whereas deletion of ADF4 impairs the disassembly of actin filaments and causes stomatal closure to be more sensitive to ABA. Deletion of ADF4 in the ckl2 mutant partially recues its ABA-insensitive stomatal closure phenotype. Moreover, Arabidopsis ADFs from subclass I are targets of CKL2 in vitro. Thus, our results suggest that CKL2 regulates actin filament reorganization and stomatal closure mainly through phosphorylation of ADF.

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Section: Ckl2 Colocalizes With Actin Filaments In Cells But Does Not mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

CASEIN KINASE1-LIKE PROTEIN2 Regulates Actin Filament Stability and Stomatal Closure via Phosphorylation of Actin Depolymerizing Factor

et al. 2016

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“…In Arabidopsis and lily (Lilium longiflorum), fimbrins (FIM), domain of Lin11, Isl-1, and Mec-3 proteins, actin-depolymerizing factors, several formins, and villins (VLN) have been shown to be involved in the formation and maintenance of longitudinal actin cables in the shank region of pollen tubes Zheng et al, 2013;Fu, 2015). VLN2, VLN5, AtFIM5, and LlFIM1 bundle apical PM-anchored F-actin and contribute to the formation of actin fringe or collar structures in the subapical region (Su et al, 2012;Qu et al, 2013;Zhang et al, 2016a). In the apex, several actin-binding proteins, including VLN and Ropinteractive CRIB Motif-containing Protein1, are implicated in the patterning of apical F-actin dynamics Zhou et al, 2015).…”

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confidence: 99%

Rice Morphology Determinant-Mediated Actin Filament Organization Contributes to Pollen Tube Growth

Yang

Zhang

et al. 2018

Plant Physiol.

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“…The reorganization of actin filaments is regulated by an array of actin binding proteins, such as monomer binding protein profilin, side binding protein fimbrin, actin bundling protein villin, and actin depolymerizing factor (ADF) [1,2,[7][8][9]. ADF proteins have ability to bind both G-actin monomer and F-actin filament, and modulate actin filament reorganization through depolymerizing filaments from their pointed ends, severing actin filaments, and increasing the concentration of barbed ends for promotion of actin polymerization [8][9][10][11][12].…”

mentioning

confidence: 99%

“…As one of major cytoskeleton networks, actin filaments constantly undergo rapid reorganization in plant cells, which is required for variety of cellular processes ranging from cell division, morphogenesis, elongation, tip growth and cell movement and plays an important role in plant growth, development and stress responses [1][2][3][4][5][6]. The reorganization of actin filaments is regulated by an array of actin binding proteins, such as monomer binding protein profilin, side binding protein fimbrin, actin bundling protein villin, and actin depolymerizing factor (ADF) [1,2,[7][8][9].…”

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confidence: 99%

14-3-3 λ protein interacts with ADF1 to regulate actin cytoskeleton dynamics in Arabidopsis

Zhao

Guo

2015

Sci. China Life Sci.

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Actin cytoskeleton dynamics is critical for variety of cellular events including cell elongation, division and morphogenesis, and is tightly regulated by numerous groups of actin binding proteins. However it is not well understood how these actin binding proteins are modulated in a physiological condition by their interaction proteins. In this study, we describe that Arabidopsis 14-3-3  protein interacted with actin depolymerizing factor 1 (ADF1) in plant to regulate F-actin stability and dynamics. Loss of 14-3-3  in Arabidopsis resulted in longer etiolated hypocotyls in dark and changed actin cytoskeleton architecture in hypocotyl cells. Overexpression of ADF1 repressed 14-3-3  mutant hypocotyl elongation and actin dynamic phenotype. In addition, the phosphorylation level of ADF1 was increased and the subcellular localization of ADF1 was altered in 14-3-3  mutant. Consistent with these observations, the actin filaments were more stable in 14-3-3  mutant. Our results indicate that 14-3-3  protein mediates F-actin dynamics possibly through inhibiting ADF1 phosphorylation in vivo.Arabidopsis, 14-3-3, ADF, phosphorylation, actin cytoskeleton dynamics Citation:Zhao SS, Zhao YX, Guo Y. 14-3-3  protein interacts with ADF1 to regulate actin cytoskeleton dynamics in

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FIMBRIN1 Is Involved in Lily Pollen Tube Growth by Stabilizing the Actin Fringe

Cited by 59 publications

References 63 publications

CASEIN KINASE1-LIKE PROTEIN2 Regulates Actin Filament Stability and Stomatal Closure via Phosphorylation of Actin Depolymerizing Factor

CASEIN KINASE1-LIKE PROTEIN2 Regulates Actin Filament Stability and Stomatal Closure via Phosphorylation of Actin Depolymerizing Factor

Rice Morphology Determinant-Mediated Actin Filament Organization Contributes to Pollen Tube Growth

14-3-3 λ protein interacts with ADF1 to regulate actin cytoskeleton dynamics in Arabidopsis

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