FimA, FimF, and FimH Are Necessary for Assembly of Type 1 Fimbriae on Salmonella enterica Serovar Typhimurium

Zeiner, Sarah A.; Dwyer, Brett E.; Clegg, Steven

doi:10.1128/iai.00331-12

Cited by 59 publications

(36 citation statements)

References 46 publications

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“…Choleraesuis was unable to produce type 1 fimbriae without FimH. This finding is in line with the report of Zeiner et al that indicated that FimA, FimF, and FimH are necessary for the assembly of type 1 fimbriae in Salmonella [ 7 ].…”

Section: Discussionsupporting

confidence: 90%

“…The phenotypic expression of type 1 fimbriae is primarily a result of cooperation among several Fim proteins and arginine tRNA encoded by fimU within the fim gene cluster. FimA, FimI, FimH, and FimF are fimbrial subunits that form the shaft of type 1 fimbriae, with FimA as the major fimbrial subunit [ 7 ]. Type 1 fimbriae adhere to a variety of cells that possess mannose residues, such as erythrocytes, leukocytes, yeast, and respiratory cells [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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The Non-Fimbriate Phenotype Is Predominant among Salmonella enterica Serovar Choleraesuis from Swine and Those Non-Fimbriate Strains Possess Distinct Amino Acid Variations in FimH

Lee

Yeh

2016

PLoS ONE

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Although most Salmonella serovars are able to infect a range of animal hosts, some have acquired the ability to cause systemic infections of specific hosts. For example, Salmonella enterica serovar Choleraesuis is primarily associated with systemic infection in swine. Adherence to host epithelial cells is considered a prerequisite for initial infection, and fimbrial appendages on the outer membrane of the bacteria are implicated in this process. Although type 1 fimbriae encoded by the fim gene cluster are commonly found in Salmonella serovars, it is not known whether S. Choleraesuis produces this fimbrial type and if and how fimbriae are involved in pathogenesis. In the present study, we demonstrated that only four out of 120 S. Choleraesuis isolates from pigs with salmonellosis produced type 1 fimbriae as assayed by the yeast agglutination test and electron microscopy. One of the 116 non-type 1 fimbria-producing isolates was transformed with plasmids carrying different fim genes from S. Typhimurium LB5010, a type 1 fimbria-producing strain. Our results indicate that non-type 1 fimbria-producing S. Choleraesuis required only an intact fimH to regain the ability to produce fimbrial appendages. Sequence comparison revealed six amino acid variations between the FimH of the non-type 1 fimbria-producing S. Choleraesuis isolates and those of the type 1 fimbria-producing S. Choleraesuis isolates. S. Choleraesuis that produced type 1 fimbriae contained FimH with an amino acid sequence identical to that of S. Typhimurium LB5010. Site-directed mutagenesis leading to the replacement of the non-conserved residues revealed that a change from glycine to valine at position of 63 (G63V) resulted in a non-type 1 fimbria-producing S. Choleraesuis being able to express type 1 fimbriae on its outer membrane. It is possible that this particular amino acid change prevents this polypeptide from proper interaction with other Fim subunits required for assembly of an intact type 1 fimbrial shaft in S. Choleraesuis; however, it remains to be determined if and how the absence of type 1 fimbriae production is related to the systemic infection of the swine host by S. Choleraesuis.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

The Non-Fimbriate Phenotype Is Predominant among Salmonella enterica Serovar Choleraesuis from Swine and Those Non-Fimbriate Strains Possess Distinct Amino Acid Variations in FimH

Lee

Yeh

2016

PLoS ONE

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“…Another gene cluster that was ubiquitously present were the fim genes that encodes for type 1 fimbriae. This cluster is comprised of the fimAICDHF operon and three regulatory genes fimW, fimY, and fimZ and mediates adherence to eukaryotic cells [ 21 ]. However, the fimY gene was not detected in ten isolates at the BLAST search cut-off level we used.…”

Section: Resultsmentioning

confidence: 99%

Whole genome sequencing-based detection of antimicrobial resistance and virulence in non-typhoidal Salmonella enterica isolated from wildlife

et al. 2017

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The aim of this study was to generate a reference set of Salmonella enterica genomes isolated from wildlife from the United States and to determine the antimicrobial resistance and virulence gene profile of the isolates from the genome sequence data. We sequenced the whole genomes of 103 Salmonella isolates sampled between 1988 and 2003 from wildlife and exotic pet cases that were submitted to the Oklahoma Animal Disease Diagnostic Laboratory, Stillwater, Oklahoma. Among 103 isolates, 50.48% were from wild birds, 0.9% was from fish, 24.27% each were from reptiles and mammals. 50.48% isolates showed resistance to at least one antibiotic. Resistance against the aminoglycoside streptomycin was most common while 9 isolates were found to be multi-drug resistant having resistance against more than three antibiotics. Determination of virulence gene profile revealed that the genes belonging to csg operons, the fim genes that encode for type 1 fimbriae and the genes belonging to type III secretion system were predominant among the isolates. The universal presence of fimbrial genes and the genes encoded by pathogenicity islands 1–2 among the isolates we report here indicates that these isolates could potentially cause disease in humans. Therefore, the genomes we report here could be a valuable reference point for future traceback investigations when wildlife is considered to be the potential source of human Salmonellosis.Electronic supplementary materialThe online version of this article (10.1186/s13099-017-0213-x) contains supplementary material, which is available to authorized users.

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“…The selection of an appropriate target gene for Salmonella detection is key to developing a great visualized LAMP method. Salmonella possesses many types of pili; type I pili are widely distributed among various serotypes, and the fimW gene is one of the major regulatory genes of type I pilus subunit proteins ( Supreet et al., 2009 , Zeiner et al., 2012 ). A search of sequences in GenBank using the fimW gene sequence as a query showed that fimW is a unique gene that is only present in different Salmonella serotypes, and no homologous sequences were found in other pathogens; therefore, we selected the fimW gene as an ideal target for the method.…”

Section: Discussionmentioning

confidence: 99%

A rapid novel visualized loop-mediated isothermal amplification method for Salmonella detection targeting at fimW gene

et al. 2020

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Salmonella infection causes huge losses in the poultry industry worldwide. With the aim to prevent infectious diseases caused by Salmonella and to achieve rapid visualized Salmonella detection in poultry production, we used cresol red as an indicator to develop a novel visualized loop-mediated isothermal amplification method that targets the Salmonella fimW gene firstly in related field. The detection limit was 7.3 × 10 1 CFU/mL, and the method was highly specific and showed a high clinical detection rate. The entire reaction can be completed in about 40 min and only requires a water bath at 62°C, which makes the method extremely suitable for application to poultry production.

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FimA, FimF, and FimH Are Necessary for Assembly of Type 1 Fimbriae on Salmonella enterica Serovar Typhimurium

Abstract: ABSTRACTSalmonella entericaserovar Typhimurium is a Gram-negative member of the familyEnterobacteriaceaeand is a common cause of bacterial food poisoning in humans. The fimbrial appendages are found on the surface of… Show more

Cited by 59 publications

References 46 publications

The Non-Fimbriate Phenotype Is Predominant among Salmonella enterica Serovar Choleraesuis from Swine and Those Non-Fimbriate Strains Possess Distinct Amino Acid Variations in FimH

The Non-Fimbriate Phenotype Is Predominant among Salmonella enterica Serovar Choleraesuis from Swine and Those Non-Fimbriate Strains Possess Distinct Amino Acid Variations in FimH

Whole genome sequencing-based detection of antimicrobial resistance and virulence in non-typhoidal Salmonella enterica isolated from wildlife

A rapid novel visualized loop-mediated isothermal amplification method for Salmonella detection targeting at fimW gene

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