Filtration of protein-based solutions with ceramic ultrafiltration membrane. Study of selectivity, adsorption, and protein denaturation

Miron, Simona; Dutournié, Patrick; Thabet, Kawther; Ponche, Arnaud

doi:10.1016/j.crci.2018.09.011

Cited by 14 publications

(8 citation statements)

References 31 publications

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“…The results show that the membranes have an apparent different pore size distribution (different rejection rates of vitamin B12). In a previous study 4 , it was observed that during the ultrafiltration of lysozyme, there was a decrease in the hydraulic performances of the membrane, while there was an increase in the selectivity. This behavior was related to a phenomenon of protein adsorption.…”

Section: Resultsmentioning

confidence: 92%

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Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme

Miron

Espindola

Dutournié

et al. 2021

Sci Rep

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During the processing of biomolecules by ultrafiltration, the lysozyme enzyme undergoes conformational changes, which can affect its antibacterial activity. Operational conditions are considered to be one of the main parameters responsible for such changes, especially when using the same membrane and molecule. The present study demonstrates that, the same cut-off membrane (commercial data) can result in different properties of the protein after filtration, due to their different pore network. The filtration of lysozyme, regardless of the membrane, produces a decrease in the membrane hydraulic permeability (between 10 and 30%) and an increase in its selectivity in terms of observed rejection rate (30%). For the filtrated lysozyme, it appears that the HPLC retention time increases depending on the membrane used. The antibacterial activity of the filtrated samples is lower than the native protein and decreases with the increase of the applied pressure reaching 55–60% loss for 12 bar which has not been reported in the literature before. The observed results by SEC-HPLC and bacteriological tests, suggest that the conformation of the filtrated molecules are indeed modified. These results highlight the relationship between protein conformation or activity and the imposed shear stress.

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Section: Resultsmentioning

confidence: 92%

“…Some of applied unitary operations are purification, concentration, mixing, pumping, heating, and delivery. These procedure steps can induce protein denaturation and as a consequence the loss of biological activity 4 , 5 . The common technique used to guarantee good quality of products is to add additives, preservatives, buffer, etc.…”

Section: Introductionmentioning

confidence: 99%

Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme

Miron

Espindola

Dutournié

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The three raw membranes have no lysozyme ltration history, while the others have performed lysozyme ltrations before cleaning process. In a previous study [4], it was observed that during the ultra ltration of lysozyme regardless of the membrane used, there is a decrease in the hydraulic performances of the membrane, while there is an increase in the selectivity. This behavior is related to a phenomenon of protein adsorption.…”

Section: Filtration Studymentioning

confidence: 93%

“…These procedure steps can induce protein denaturation and as consequence the loss of biological activity. [4,5]. The commonly technique used to guarantee good quality of products is to add additives, preservatives, buffer, etc.…”

Section: Introductionmentioning

confidence: 99%

Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme

Miron

Espindola

Dutournié

et al. 2021

Preprint

Self Cite

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During the processing of biomolecules by ultrafiltration, the lysozyme enzyme suffers conformational changes, which can affect its antibacterial activity. Operational conditions are considered ones of the principal responsible for the modifications, especially when using the same membrane and molecule. The present study demonstrates that, the same cut-off membrane (commercial data) can produce different properties on the protein after filtration, due to their different pore network. The filtration of lysozyme, regardless of the membrane, produces a decrease in the membrane hydraulic permeability (between 10–30%) and an increase in its selectivity in terms of observed rejection rate (30%). For the filtrated lysozyme, it appears that the HPLC retention time increases depending on the membrane used. The antibacterial activity of the filtrated samples is lower than the native protein and decreases with the increase of the applied pressure reaching 55–60% loss for 12 bar which is not reported in the literature. The observed results by SEC-HPLC and bacteriological tests, suggest that the conformation of the filtrated molecules are indeed modified. These results highlight the relationship between protein conformation or activity and the imposed shear stress.

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“…The ultrafiltration is a commonly used approach for protein separation, fractionation, and purification. However, adsorption, molecule aggregation, and denaturation are the main problems that a scientist faces during the ultrafiltration process of proteins in the native form [21,22]. Aggregation is a general term that encompasses several types of interactions or characteristics.…”

Section: Purification Of Animal Proteins By Membrane Techniquesmentioning

confidence: 99%

Technological approaches to the extraction and purification by ultrafiltration techniques of target protein molecules from animal tissues: a review

Kotenkova

Polishchuk

2022

Teor. prakt. pererab. mâsa (Print)

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Effective isolation and purification of protein is a great challenge nowadays. The key aspect is protein stability and solubility, which primarily depend on protein structure and its amino acid sequence. Manipulations with pH and ionic strength are the first at tempts to increase protein stability and solubility. Different additives that are allowed or prohibited in the food industry are applied for overcoming protein aggregation. Sugars, polyhydric alcohols and amino acids are the most attractive among them. Trehalose, glycerol, arginine, glycine and proline demonstrated outstanding properties that make them perspective for application during iso lation and purification of proteins singly or in combination with each other or othercompounds. However, the algorithm of effective isolation and purification of protein could be significantly varied depending on its structure.

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Filtration of protein-based solutions with ceramic ultrafiltration membrane. Study of selectivity, adsorption, and protein denaturation

Cited by 14 publications

References 31 publications

Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme

Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme

Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme

Technological approaches to the extraction and purification by ultrafiltration techniques of target protein molecules from animal tissues: a review

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