Filtered platelet concentrates from pooled buffy coats show comparable storagelesions when stored for 9 d at 20–24°C or when supplemented with ThromboSol^™at 2–6°C

Abstract

The present study investigated the quality of platelet concentrates from pooled buffy coat (PCBC) along different production steps and during storage to characterize storage lesions reflected by platelet activation, changing metabolic and cell turnover status (pH, LDH activity). These criteria were compared in conventionally stored PCBCs (20-24 degrees C, n = 8, Group I) to cold stored PCBCs (2-6 degrees C, n = 8, Group II) when supplemented with ThromboSol. Platelet activation was measured on days 1, 3, 7 and 9 by flow cytometry using fluoresceinisothiocyanate-labeled monoclonal antibodies (mAbs) against glycoprotein IIb/IIIa (CD41a, PAC-1 and LIBS-1), P-selectin (CD62P) or CD40 ligand receptor (CD40L) in combination with a phycoerythrin-labeled panspecific platelet marker against GPIb (CD42b). The platelet activation assessed with mAbs PAC-1, CD41a, LIBS-1 and CD40L showed an overall activation of 98 +/- 4% (mean value +/- 1 SD) at day 7 in both groups, except for CD62P, which was significantly lower in Group II. A storage-dependent greater platelet loss occurred in Group II compared to Group I, 42% vs. 19% (p < 0.05 on day 9). We could demonstrate that platelets stored in ThromboSol show about the same in-vitro activation as conventionally stored PCBCs, but their clinical usefulness needs to be investigated.

Manufacturing variables and hemostatic function of cold‐stored platelets: a systematic review of the literature

“…Of the 19 (58%) articles in which the CSP products were WBD, 10 (53%) articles were produced by buffy coat preparations, and nine (47%) were produced via platelet‐rich plasma (PRP) preparations . The predominant anticoagulant for WBD platelets was citrate–phosphate‐dextrose, which was reported in 10 (53%) articles (Table ) .…”

“…The time interval between platelet donation and the start of cold storage was specified in 20 (60%) of the short‐listed articles . The time interval was reported as being within 6 hours of donation in 10 articles, between 6 and 24 hours in three articles, and more than 24 hours in seven articles (Fig.…”

“…In all of the 29 (89%) articles in which RTPs were used as a comparator to CSPs, the RTPs were stored at 22 ± 2°C with agitation, but collection and storage methods varied . However, in 28 articles, the collection and storage methods for RTPs were the same as the CSP test product.…”

PLTs of Blood Group A1 donors express increased surface A antigen owing to apheresis and prolonged storage

“…Even under optimal conditions, stored PCs demonstrate a storage lesion characterized by a variety of abnormalities, among them increased expression of surface glycoproteins, 5 whereby only more sensitive methods can document the formerly assumed stability of PLT surface antigens during storage. It was shown earlier that expression of the PLT surface glycoproteins CD41a and P‐selectin are increasing in pooled PCs 6 . It is hypothesized that the amount of ABH antigen expressed on a PLT's surface might be related to its activation status.…”

Platelet in vitro assays: their correspondence with their in vivo hemostatic potential

“…Expression of activated forms of GPIIb‐IIIa have been evaluated in different PCs using a specific antibody (PAC‐1). Results of several studies indicate that PAC‐1–positive platelets are significantly increased in different platelet products, indicating that they have been preactivated . Reduced platelet aggregation should be expected for platelet products showing an enhanced binding of PAC‐1.…”