Film Thickness Dependence of Protein Adsorption from Blood Serum and Plasma onto Poly(sulfobetaine)-Grafted Surfaces

Yang, Wei; Chen, Shengfu; Cheng, Gang; Vaisocherová, Hana; Xue, Hong; Li, Wei; Zhang, Jinli; Jiang, Shaoyi

doi:10.1021/la801487f

Cited by 221 publications

(232 citation statements)

References 39 publications

Supporting

Mentioning

216

Contrasting

Unclassified

Order By: Relevance

“…The adhesion of organisms to surfaces in nature is even more complex as the surfaces are immediately conditioned by biomacromolecules, altering the surface properties for further attachment. 44,63 To summarize, we found that surface hydration and surface energy was higher for the 64 , pSBMA 30 , pHEMA and pHPMA 65 , as well as poly(HEMA-co-PEG10MA) (this study), suggest that this is a phenomenon with some generality, and our results demonstrate that this is not limited to protein fouling, but applies also to fouling of marine organisms. We note that the polymers listed above all have chains with functional side-groups.…”

Section: Biofouling Assayssupporting

confidence: 67%

“…With regard to how the antifouling properties depend of coating thickness, this has been studied for protein adsorption in a number of systems, including early and fundamental work on poly(ethylene glycol) (PEG) coatings, [27][28] and more recent work on both hydrophilic and zwitterionic polymers. [29][30] With regard to marine fouling, in particular, there appears to be few studies on how polymer layer thicknesses influence fouling behavior, apart from the well-documented studies on the influence of polymer (PDMS) thickness at the scale of tens to hundreds of micrometres on the adhesion strength of macrofouling. 22,31 In this work a series of random poly(HEMA-co-PEG10MA) copolymer films with thicknesses from 50 to 1000 Å have been prepared via surface-initiated atom transfer radical polymerization (SI-ATRP), and the influence of polymer thickness on antifouling performance against some marine fouling species was studied in detail.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hydration and Chain Entanglement Determines the Optimum Thickness of Poly(HEMA-co-PEG₁₀MA) Brushes for Effective Resistance to Settlement and Adhesion of Marine Fouling Organisms

Yandi

Mieszkin

Martin‐Tanchereau

et al. 2014

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Understanding how surface physicochemical properties influence the settlement and adhesion of marine fouling organisms is important for the development of effective and environmentally benign marine antifouling coatings. We demonstrate that the thickness of random poly(HEMA-co-PEG10MA) copolymer brushes affect antifouling behaviour.Films of thicknesses ranging from 50 to 1000 Å were prepared via surface-initiated atom-transfer radical polymerization, and characterized using infrared spectroscopy, ellipsometry, atomic force microscopy and contact angle measurements. The fouling resistance of these films was investigated by protein adsorption, attachment of the marine bacterium Cobetia marina, settlement and strength of attachment tests of zoospores of the marine alga Ulva linza, and static immersion field tests. These assays show that the polymer film thickness influenced the antifouling performance, in that there is an optimum thickness range, 200-400 Å, where fouling of all types, as well as algal spore adhesion, was lower. Field test results also showed lower fouling within the same thickness range after two weeks' immersion. Studies by quartz crystal microbalance with dissipation (QCM-D) and underwater captive bubble contact angle measurements show strong correlation between lower fouling and higher hydration, viscosity and surface energy of the poly(HEMA-co-PEG10MA) brushes at thicknesses around 200-400 Å. We hypothesize that the reduced antifouling performance is caused by a lower hydration capacity of the polymer for thinner films, and that entanglement and crowding in the film reduces the conformational freedom, hydration capacity, and fouling resistance for thicker films.3

show abstract

Section: Biofouling Assayssupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Hydration and Chain Entanglement Determines the Optimum Thickness of Poly(HEMA-co-PEG₁₀MA) Brushes for Effective Resistance to Settlement and Adhesion of Marine Fouling Organisms

Yandi

Mieszkin

Martin‐Tanchereau

et al. 2014

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…[32,43,58,59] The ''graft-to'' method involves (i) the preparation of polymers containing nonfouling and adhesive groups, such as 3,4-dihydroxyphenylalanine (DOPA) groups [24,60] and polylysine segments, [61] and (ii) the direct attachment of the polymers onto the surface. pSB and pCB have been grafted to or from surfaces via several common adhesive linkers, including thiols for a gold surface, [43,45,47,50,51,62,63] silanes for a glass surface, [64] hydrophobic moieties for a hydrophobic surface, [41,65,66] or DOPA for various surfaces, [67,68] or via interpenetrating networks (IPNs) into polyurethane (SPU) matrices. [69] pSB-based hydrogels have also been prepared.…”

Section: Ultralow Fouling Psb and Pcb Zwitterionic Materialsmentioning

confidence: 99%

“…While protein adsorption from single-protein solutions is not very sensitive to film thickness, protein adsorption from complex media such as undiluted blood plasma and serum depends on the film thickness. Protein adsorption on pSB-grafted surfaces of different film thicknesses [62] was first performed with two common proteins, i.e., fibrinogen (340 kD, pI ¼ 5.5, where pI ¼ isoelectric point) and lysozyme (14 kD, pI ¼ 12). From Figure 3a, it can be seen that all surfaces exhibit lysozyme and fibrinogen adsorption of <3 ng cm…”

Section: à2mentioning

confidence: 99%

Ultralow‐Fouling, Functionalizable, and Hydrolyzable Zwitterionic Materials and Their Derivatives for Biological Applications

2010

Self Cite

View full text Add to dashboard Cite

In recent years, zwitterionic materials such as poly(carboxybetaine) (pCB) and poly(sulfobetaine) (pSB) have been applied to a broad range of biomedical and engineering materials. Due to electrostatically induced hydration, surfaces coated with zwitterionic groups are highly resistant to nonspecific protein adsorption, bacterial adhesion, and biofilm formation. Among zwitterionic materials, pCB is unique due to its abundant functional groups for the convenient immobilization of biomolecules. pCB can also be prepared in a hydrolyzable form as cationic pCB esters, which can kill bacteria or condense DNA. The hydrolysis of cationic pCB esters into nonfouling zwitterionic groups will lead to the release of killed microbes or the irreversible unpackaging of DNA. Furthermore, mixed-charge materials have been shown to be equivalent to zwitterionic materials in resisting nonspecific protein adsorption when they are uniformly mixed at the molecular scale.

show abstract

“…pCBMA-1 C2 and control coatings were grafted by surface-initiated atom transfer radical polymerization (ATRP) onto a gold surface covered with initiators. The thicknesses of the obtained polymer coatings, as measured by atomic force microscopy (AFM), [26] were 26-32 nm (Table 1).The bactericidal activity of pCBMA-1 C2 surfaces was determined using E. coli K12, according to a modified literature procedure.[2] The permanently cationic poly(methacryloyloxyethyldimethyloctylammonium bromide) (pC8NMA, cationic control) and the zwitterionic poly (2-carboxy-N,N-dimethyl-N-[2'-(methacryloyloxy)ethyl]ethanaminium) (pCBMA-2, zwitterionic control) were used as the positive and the negative control surfaces, respectively (Scheme 1). The antimicrobial efficiency was defined as the amount of live cells on the tested surfaces relative to those on the pCBMA-2 surface.…”

mentioning

confidence: 99%

A Switchable Biocompatible Polymer Surface with Self‐Sterilizing and Nonfouling Capabilities

et al. 2008

Self Cite

View full text Add to dashboard Cite

Microbial adhesion onto implanted biomaterials and the subsequent formation of biofilms is one of the major causes of biomedical device failure. The use of antimicrobial and nonfouling coatings are two strategies for the prevention of the attachment and spreading of microorganisms on the surfaces of implantable materials. Antimicrobial surfaces containing covalently linked quaternary ammonium compounds (QACs) have proved to be able to efficiently kill a variety of microorganisms. [1][2][3][4][5][6][7] A major problem with QAC surfaces is the attachment of dead microorganisms remaining on antimicrobial coatings, which can trigger an immune response and inflammation, and block its antimicrobial functional groups. In addition, such antimicrobial coatings can not fulfill the requirements of nonfouling [8] and biocompatibility as implantable biomaterials. Poly(ethylene glycol) (PEG) derivatives [9][10][11][12][13][14] or zwitterionic polymers [15][16][17][18] have been extensively used as nonfouling materials to reduce bacterial attachment and biofilm formation. However, the susceptibility of PEG to oxidation damage has limited its long-term application in complex media. [11,19] We recently showed that zwitterionic materials such as poly(sulfobetaine methacrylate) (pSBMA) were able to dramatically reduce bacterial attachment and biofilm formation [18] and were highly resistant to nonspecific protein adsorption, even from undiluted blood plasma and serum. [20][21][22][23][24] Although zwitterionic coatings can reduce the initial attachment and delay colonization of microbes on surfaces, there is a possibility of introducing pathogenic microbes into the patient during implantation operations and catheter insertions, which results in the failure of implanted devices; the use of antimicrobial agents will then be necessary to eliminate these microbes. Surface-responsive materials have been developed for a broad spectrum of applications, [25] but it is still a great challenge to develop biocompatible materials that have both antimicrobial and nonfouling capabilities. To the best of our knowledge, no such materials have been reported to date.Herein we report a new switchable polymer surface coating, which combines the advantages of both nonfouling and cationic antimicrobial materials and overcomes their disadvantages (Figure 1). In this system, poly(N,N-dimethyl-N-(ethoxycarbonylmethyl)-N-[2'-(methacryloyloxy)ethyl]-ammonium bromide) (pCBMA-1 C2, cationic precursor) on a surface can kill greater than 99.9 % of Escherichia coli K12 in one hour, and 98 % of the dead bacterial cells can be released when the cationic derivatives are hydrolyzed to nonfouling zwitterionic polymers. pCBMA-1 C2 and control coatings were grafted by surface-initiated atom transfer radical polymerization (ATRP) onto a gold surface covered with initiators. The thicknesses of the obtained polymer coatings, as measured by atomic force microscopy (AFM), [26] were 26-32 nm (Table 1).The bactericidal activity of pCBMA-1 C2 surfaces was determined using E. co...

show abstract

Film Thickness Dependence of Protein Adsorption from Blood Serum and Plasma onto Poly(sulfobetaine)-Grafted Surfaces

Cited by 221 publications

References 39 publications

Hydration and Chain Entanglement Determines the Optimum Thickness of Poly(HEMA-co-PEG₁₀MA) Brushes for Effective Resistance to Settlement and Adhesion of Marine Fouling Organisms

Hydration and Chain Entanglement Determines the Optimum Thickness of Poly(HEMA-co-PEG₁₀MA) Brushes for Effective Resistance to Settlement and Adhesion of Marine Fouling Organisms

Ultralow‐Fouling, Functionalizable, and Hydrolyzable Zwitterionic Materials and Their Derivatives for Biological Applications

A Switchable Biocompatible Polymer Surface with Self‐Sterilizing and Nonfouling Capabilities

Contact Info

Product

Resources

About

Film Thickness Dependence of Protein Adsorption from Blood Serum and Plasma onto Poly(sulfobetaine)-Grafted Surfaces

Cited by 221 publications

References 39 publications

Hydration and Chain Entanglement Determines the Optimum Thickness of Poly(HEMA-co-PEG10MA) Brushes for Effective Resistance to Settlement and Adhesion of Marine Fouling Organisms

Hydration and Chain Entanglement Determines the Optimum Thickness of Poly(HEMA-co-PEG10MA) Brushes for Effective Resistance to Settlement and Adhesion of Marine Fouling Organisms

Ultralow‐Fouling, Functionalizable, and Hydrolyzable Zwitterionic Materials and Their Derivatives for Biological Applications

A Switchable Biocompatible Polymer Surface with Self‐Sterilizing and Nonfouling Capabilities

Contact Info

Product

Resources

About

Hydration and Chain Entanglement Determines the Optimum Thickness of Poly(HEMA-co-PEG₁₀MA) Brushes for Effective Resistance to Settlement and Adhesion of Marine Fouling Organisms

Hydration and Chain Entanglement Determines the Optimum Thickness of Poly(HEMA-co-PEG₁₀MA) Brushes for Effective Resistance to Settlement and Adhesion of Marine Fouling Organisms