This work presents a numerical investigation of the combined effects of thermal barrier coating (TBC) with mainstream turbulence intensity (Tu) on a modified vane of the real film-cooled nozzle guide vane (NGV) reported by Timko (NASA CR-168289). Using a 3D conjugate heat transfer (CHT) analysis, the NGVs with and without TBC are simulated at three Tus (Tu = 3.3%, 10% and 20%). The overall cooling effectiveness, TBC effectiveness and heat transfer coefficient are analyzed and discussed. The results indicate the following three interesting phenomena: (1) TBC on the pressure side (PS) is more effective than that on the suction side (SS) due to a fewer number of film holes on the SS; (2) for all three Tus, the variation trends of the overall cooling effectiveness are similar, and TBC plays the positive and negative roles in heat flux at the same time, and significantly increases the overall cooling effectiveness in regions cooled ineffectively by cooling air; (3) when Tu increases, the TBC effect is more significant, for example, at the highest Tu (Tu = 20%) the overall cooling effectiveness can increase as much as 24% in the film cooling ineffective regions, but near the trailing edge (TE) and the exits and downstream of film holes on the SS, this phenomenon is slight.