Filamin A upregulation correlates with Snail-induced epithelial to mesenchymal transition (EMT) and cell adhesion but its inhibition increases the migration of colon adenocarcinoma HT29 cells

Wieczorek, Katarzyna; Wiktorska, Magdalena; Sacewicz-Hofman, Izabela; Boncela, Joanna; Lewiński, Andrzej; Kowalska, M. Anna; Niewiarowska, Jolanta

doi:10.1016/j.yexcr.2017.07.035

Cited by 18 publications

(12 citation statements)

References 38 publications

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“…Its overexpression is generally correlated with aggressiveness, lymph node metastases (LNM), tumor recurrence and poor prognosis [15,16]. Our previous experiments concerning colon adenocarcinoma HT29 cells also indicated that Snail-1 overexpression reduced cell adhesion and increased migratory properties, which coincides with the above observations [17]. Furthermore, it was shown that Snail-1 may also play a key role in cancer cell cytoskeleton reorganization, while a positive correlation between Snail-1 presence and Tubulin-β3, (TUBB3) (one of the main proteins building microtubules, a major component of the eukaryotic cytoskeleton) upregulation in adenocarcinoma cell lines has been observed [18].…”

Section: Introductionsupporting

confidence: 72%

Snail-1 Overexpression Correlates with Metastatic Phenotype in BRAFV600E Positive Papillary Thyroid Carcinoma

Wieczorek-Szukała

Kopczyński

Kowalska

et al. 2020

JCM

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The ability of cancer to metastasize is regulated by various signaling pathways, including transforming growth factor β (TGFβ), also implicated in the upregulation of Snail-1 transcription factor in malignant neoplasms. B-type Raf kinase gene (BRAF)V600E, the most common driving mutation in papillary thyroid carcinoma (PTC), induces epithelial to mesenchymal transition (EMT) in thyroid cancer cells through changes in the Snail-1 level, increasing cell migration and invasion. However, little is known about the mechanism of Snail-1 and BRAFV600E relations in humans. Our study included 61 PTC patients with evaluated BRAFV600E mutation status. A total of 18 of those patients had lymph node metastases—of whom 10 were BRAFV600E positive, and 8 negative. Our findings indicate that the expression of Snail-1, but not TGFβ1, correlates with the metastatic phenotype in PTC. This is the first piece of evidence that the upregulation of Snail-1 corresponds with the presence of BRAFV600E mutation and increased expression of Snail-1 in metastatic PTC samples is dependent on BRAFV600E mutation status.

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Section: Introductionsupporting

confidence: 72%

Snail-1 Overexpression Correlates with Metastatic Phenotype in BRAFV600E Positive Papillary Thyroid Carcinoma

Wieczorek-Szukała

Kopczyński

Kowalska

et al. 2020

JCM

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“…Filamin A silencing increases cell adhesion and decreased migration of the bronchial carcinoid H727 cells (Vitali et al, 2017). In contrast, silencing of Filamin A inhibits Snail-induced adhesion and increases migration of colon adenocarcinoma HT29 cells (Wieczorek et al, 2017). In accordance with these results, Filamin A is required to mediate SST2 effects on adhesion and migration of the pancreatic endocrine QGP1 cells (Vitali et al, 2016).…”

Section: Filamin a In Cell Adhesion And Migrationsupporting

confidence: 56%

Molecular Tuning of Filamin A Activities in the Context of Adhesion and Migration

Lamsoul

Dupré

Lutz

2020

Front. Cell Dev. Biol.

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The dynamic organization of actin cytoskeleton meshworks relies on multiple actin-binding proteins endowed with distinct actin-remodeling activities. Filamin A is a large multi-domain scaffolding protein that cross-links actin filaments with orthogonal orientation in response to various stimuli. As such it plays key roles in the modulation of cell shape, cell motility, and differentiation throughout development and adult life. The essentiality and complexity of Filamin A is highlighted by mutations that lead to a variety of severe human disorders affecting multiple organs. One of the most conserved activity of Filamin A is to bridge the actin cytoskeleton to integrins, thereby maintaining the later in an inactive state. We here review the numerous mechanisms cells have developed to adjust Filamin A content and activity and focus on the function of Filamin A as a gatekeeper to integrin activation and associated adhesion and motility.

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“…Our three events were also present in the EMT-associated submodule extracted by applying stringent cutoffs (gene log2 fold change > 2 and AS | dPSI | > 0.25) (Figure 3G). Our network revealed the functional interactions of TCF7L2 and FGFR2 with FLNA to be a network hub and to regulate EMT in tumor cells [36]. Occurrence of the representative three AS events in the genomic regions lead to changes in the protein domain, and these were shown to be strongly involved in EMT-associated functions based on previous literature [16,37,38].…”

Section: Resultsmentioning

confidence: 71%

ASpediaFI: Functional interaction analysis of alternative splicing events

Yu¹,

Lee²,

Hyung³

et al. 2020

Preprint

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Alternative splicing (AS) regulates biological process governing phenotype or disease. However, it is challenging to systemically analyze global regulation of AS events, their gene interactions, and functions. Here, we introduce a novel application, ASpediaFI for identifying AS events and co-regulated gene interactions implicated in pathways. Our method establishes an interaction network including AS events, performs random walk with restart, and finally identifies a functional subnetwork containing the AS event. We validated the capability of ASpediaFI to interpret biological relevance based on three case studies. Using simulation data, we achieved higher accuracy than with other methods and detected pathway-associated AS events.

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Filamin A upregulation correlates with Snail-induced epithelial to mesenchymal transition (EMT) and cell adhesion but its inhibition increases the migration of colon adenocarcinoma HT29 cells

Cited by 18 publications

References 38 publications

Snail-1 Overexpression Correlates with Metastatic Phenotype in BRAFV600E Positive Papillary Thyroid Carcinoma

Snail-1 Overexpression Correlates with Metastatic Phenotype in BRAFV600E Positive Papillary Thyroid Carcinoma

Molecular Tuning of Filamin A Activities in the Context of Adhesion and Migration

ASpediaFI: Functional interaction analysis of alternative splicing events

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