Filamentous virus decoration with gold nanoparticles: global fingerprints of bionanocomposites acquired with SERS

Montalvan-Sorrosa, Danai; González-Solís, JL; Mas‐Oliva, Jaime; Castillo, Rolando

doi:10.1039/c4ra10656c

Cited by 8 publications

(25 citation statements)

References 37 publications

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“…Sample preparation for SERS only requires the addition of noble metal nanoparticles (AuNP's) to the different fractions to obtain enhanced signals by several orders of magnitude . Taking advantage of the potential of SERS for membrane studies and the characterization of bionanocomposites, we used this methodology to analyze the sphingomyelin/cholesterol‐rich domains of the plasma membrane fractions isolated from liver cells (fractions F5–F12). We employed this method in conjunction with PCA because the very small volumes of sample needed and no requirements for labeling.…”

Section: Resultsmentioning

confidence: 99%

“…Synthesis of gold nanoparticles (AuNPs) was performed as reported elsewhere and achieved by mixing 30 ml of a solution of gold hydrochloride (Sigma‐Aldrich) with the same volume of a preheated sodium citrate (Sigma‐Aldrich) solution used as reducing agent, employing gentle stirring and a temperature of 100 °C. Both solutions were used at the same concentration (0.5 mM) and prepared with ultrapure water.…”

Section: Methodsmentioning

confidence: 99%

“…For SERS, samples were prepared by adding freshly prepared 30 nm AuNPs to each fraction (3:1 v /v). Mixtures were vigorously agitated and maintained at RT for 12–24 h . A drop of sample (10 μl) was placed in a CaF 2 slide and spectra collected as fast as possible to avoid drop evaporation.…”

Section: Methodsmentioning

confidence: 99%

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Label‐free surface‐enhanced Raman spectroscopy of lipid‐rafts from hepatocyte plasma membranes

Delgado–Coello

Montalvan-Sorrosa

Cruz-Rangel

et al. 2017

J Raman Spectroscopy

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Lipid rafts are sphingomyelin/cholesterol-rich domains present in the plasma membrane of eukaryotic cells. In the hepatocyte, it has been shown that these domains intervene and modify a wide range of functions from which cell signaling in health and disease is of major importance. The present investigation proposes a novel strategy for the study of plasma membrane lipid rafts using surface-enhanced Raman spectroscopy (SERS) and spectra processing employing the principal component analysis (PCA) in correlation with conventional biochemical techniques. SERS has been used for artificial membranes; our approach has the advantage that allows studying purified biological membranes using small volumes of biological samples and a very simple protocol. Therefore, the use of SERS/PCA represents an important advantage for the study not only of the structure and composition of biological membranes but also for the understanding of the functions carried by the membrane-embedded proteins. Our work using SERS/PCA for the first time shows a correlation when studying the composition of lipid rafts from the cell plasma membrane and the catalytic activity of the Ca 2+ -ATPase, and its direct association with the presence of specific lipids located in membrane lipid rafts. Also, we show that changes in plasma membrane and the catalytic activity of the Ca 2+ -ATPase activity present in the plasma membrane of hepatocytes after partial depletion of membrane cholesterol carried out by methyl-beta-cyclodextrin correlate with the changes in the enzyme activity and the presence of cholesterol-rich domains of lipid rafts.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Label‐free surface‐enhanced Raman spectroscopy of lipid‐rafts from hepatocyte plasma membranes

Delgado–Coello

Montalvan-Sorrosa

Cruz-Rangel

et al. 2017

J Raman Spectroscopy

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“…El hígado es un órgano esencial encargado de una gran diversidad de funciones, entre las de tipo sintéticas destacan, la síntesis de albúmina, el metabolismo de lípidos y carbohidratos; también lleva a cabo la secreción de ácidos biliares y la eliminación (detoxificación) de ciertos químicos. En etapas fetales, el hígado participa además en hematopoiesis y en la homeostasis de calcio (Delgado-Coello y Mas- Oliva, 2014). El hígado recibe un doble aporte sanguíneo, el 75% proveniente de los intestinos, del bazo y del páncreas a través de la vena porta y el 25% del suministro sanguíneo de la arteria hepática, el cual contribuye a la intercomunicación del hígado con estos órganos que es fundamental para realizar sus funciones.…”

Section: Generalidades De La Anatomía Y Fisiología Hepáticaunclassified

Avances en el estudio experimental de la Bioquímica Hepática

Coello¹,

Oliva²

2017

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“… 20 , 28 − 31 Therefore, by exploring the well-known affinity of the thiol group for gold, M13 viruses modified with thiol groups (M13-SH) have been used as the efficient templates for gold reduction or assembling of preformed gold nanoparticles. 19 , 20 , 28 , 30 , 32 Convenience, versatility of chemical modifications and diverse metal affinity of the thiol groups are the advantages of M13-SH for gold extraction. 19 , 20 , 28 , 30 , 32 …”

Section: Introductionmentioning

confidence: 99%

Bioreduction of Gold Ions under Greener Conditions by the Thiol-Modified M13 Bacteriophage and with Hydroxylamine as the Autocatalytic Reducing Agent

et al. 2022

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Bioreduction of gold ions by the thiol-modified M13 bacteriophage (M13-SH) has been exploited as the potential alternative to conventional methods based on toxic chemicals, due to the gold affinity of the thiol groups, inherent gold reduction, and high specific surface area of the filamentous virus. Such efforts have been hindered by harsh conditions involving strong reducing agents and extreme pH that are harmful to the virus. Herein, a virus-friendly and greener method of bioreduction of AuCl 4 – at neutral pH based on M13-SH is demonstrated. M13-SH was prepared by coupling the virus with N -succinimidyl S -acetylthioacetate, followed by deacylation in the presence of hydroxylamine·HCl to expose the thiol groups. The key finding is that without time-consuming purification, the mixture after deacylation consisting of M13-SH, residual hydroxylamine, and so forth can directly turn ionic gold species into gold, leading to macroscopic precipitated products with interconnected linear structures consisting of fused gold nanoparticles. Besides working as the virus-friendly reducing agent with a unique autocatalytic style, hydroxylamine diminishes disulfide bonding-induced intervirus bundling of M13-SH so as to maintain its efficient biosorption of ionic gold precursors. This work demonstrates a general and green strategy of bioreduction of gold via combination of the gold-affinity proteins or organisms and the unique autocatalytic reduction of hydroxylamine.

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Filamentous virus decoration with gold nanoparticles: global fingerprints of bionanocomposites acquired with SERS

Abstract: A bioconjugation reaction is used to obtain fd viruses with one gold nanoparticle at the tip and gold nanowire-like structures.

Cited by 8 publications

References 37 publications

Label‐free surface‐enhanced Raman spectroscopy of lipid‐rafts from hepatocyte plasma membranes

Label‐free surface‐enhanced Raman spectroscopy of lipid‐rafts from hepatocyte plasma membranes

Avances en el estudio experimental de la Bioquímica Hepática

Bioreduction of Gold Ions under Greener Conditions by the Thiol-Modified M13 Bacteriophage and with Hydroxylamine as the Autocatalytic Reducing Agent

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