Filamentous Alphaproteobacteria Associated with Bulking in Industrial Wastewater Treatment Plants

Levantesi, Caterina; Beimfohr, Claudia; Geurkink, Bert; Rossetti, Simona; Thelen, Karin; Krooneman, Janneke; Snaidr, Jiri; Waarde, J. van der; Tandoi, Valter

doi:10.1078/0723202042369974

Cited by 110 publications

(84 citation statements)

References 25 publications

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“…They were present in about 65% of 86 industrial WWTPs investigated in former studies [26] but their broad distribution would suggest that none of the filamentous Alphaproteobacteria was preferentially associated to a plant treating a particular industrial waste [26]. However in the present case they seem to come with the treated effluent.…”

Section: Inoculum and Wastewater Compositionmentioning

confidence: 51%

Filamentous bacteria existence in aerobic granular reactors

Figueroa

Río

Campos

et al. 2014

Bioprocess Biosyst Eng

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Filamentous bacteria are associated to biomass settling problems in wastewater treatment plants. In systems based on aerobic granular biomass they have been proposed to contribute to the initial biomass aggregation process. However their development on mature aerobic granular systems has not been sufficiently studied. In the present research work filamentous bacteria were studied for the first time after long term operation (up to 300 days) of aerobic granular systems. Chloroflexi and Sphaerotilus natans have been observed in a reactor fed with synthetic wastewater. These filamentous bacteria could only come from the inoculated sludge. Thiothrix and Chloroflexi bacteria were observed in aerobic granular biomass treating wastewater from a fish canning industry. Meganema perideroedes was detected in a reactor treating wastewater from a plant processing marine products. As a conclusion the source of filamentous bacteria in these mature aerobic granular systems fed with industrial effluents was the incoming wastewater.

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Section: Inoculum and Wastewater Compositionmentioning

confidence: 51%

Filamentous bacteria existence in aerobic granular reactors

Figueroa

Río

Campos

et al. 2014

Bioprocess Biosyst Eng

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“…Amplification of 16S rRNA genes by PCR, purification of products and sequencing were performed as described by Levantesi et al (2004). The sequences were edited using Sequencer DNA sequencing software (Gene Codes Inc.).…”

Section: Methodsmentioning

confidence: 99%

Identity, abundance and ecophysiology of filamentous bacteria belonging to the Bacteroidetes present in activated sludge plants

Kragelund

Levantesi

Borger³

et al. 2008

Microbiology

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Filamentous members of the Bacteroidetes are commonly observed in activated sludge samples originating from both municipal and industrial wastewater treatment plants (WWTP), where they occasionally can cause bulking. Several oligonucleotide 16S rRNA-targeted probes were designed to target filaments with a needle-like appearance similar to Haliscomenobacter hydrossis. The design of these probes was based on an isolate and a sequence obtained from a micromanipulated filament. The abundance of filamentous Bacteroidetes was determined in 126 industrial samples applying already published and the newly developed probes. Small populations were found in 62 % of the WWTP investigated. However, only relatively few WWTP (13 %) contained large populations of filamentous Bacteroidetes potentially responsible for bulking incidences. The identity of the most abundant filamentous Bacteroidetes with H. hydrossis morphology could be detected by probes CFB719, SAP-309 and the newly designed probe HHY-654. A comprehensive study on the ecophysiology of probe-defined Bacteroidetes populations was conducted on Danish and Czech samples. The studies revealed that they were specialized bacteria involved in degradation of sugars, e.g. glucose and N-acetylglucosamine, and may participate in the conversion of lipopolysaccharides and peptidoglycan liberated by decaying cells. Many surface-associated exo-enzymes were excreted, e.g. chitinase, glucuronidase, esterase and phosphatase, supporting conversion of polysaccharides and possibly other released cell components. The role of filamentous bacteria with a H. hydrossis-like morphology in the activated sludge ecosystem is discussed.

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“…Two probes were designed for the strains isolated in this study: S-Ss-Meg-983-a-A-19 (Meg983), 59-CGGGATGTCAAAAGGTGG-39, and S-Ss-Meg-1028-a-A-19 (Meg1028), 59-CTGTCACCGAGTCCCTTGC-39. Both probes targeted the novel strains perfectly, as well as the strains described by Levantesi et al (2004). Probe binding profiles of hybridized pure cultures (Gr1 T , Gr2 and Gr10) and Grindsted sludge samples showed probe Meg983 hybridized well up to 35 % formamide, while the intensity of probe Meg1028 decreased above 50 % formamide.…”

mentioning

confidence: 99%

“…The closest relative was Brevundimonas vesicularis LMG 2350 T , which showed 89?8 % identity with strain Gr1 T . Levantesi et al (2004) reported on other filamentous alphaproteobacteria. They are so far described only by 16S rRNA gene sequences from uncultured bacteria or from isolates that have not yet been described.…”

mentioning

confidence: 99%

Meganema perideroedes gen. nov., sp. nov., a filamentous alphaproteobacterium from activated sludge

Thomsen

Blackall

Muro

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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Meganema perideroedes gen. nov., sp. nov., a filamentous alphaproteobacterium from activated sludge An industrial wastewater treatment plant at Grindsted, Denmark, has suffered from bulking problems for several years caused by filamentous bacteria. Five strains were isolated from the sludge by micromanipulation. Phylogenetic analysis of the 16S rRNA gene sequences showed that the strains formed a monophyletic cluster in the Alphaproteobacteria, and they were phenotypically different from their closest relatives and from all hitherto known filamentous bacteria described (closest relative Brevundimonas vesicularis ATCC 11426 T , 89?8 % sequence similarity). In pure culture, the cells (1?5-2?0 mm) in filaments are Gram-negative and contain polyphosphate and polyhydroxyalkanoates. The optimum temperature for growth is 30 6C and the strains grow in 2 % NaCl and are oxidase-and catalase-positive. Ubiquinone 10 is the major quinone. The major fatty acid (C 18 : 1 v7c) and smaller amounts of unsaturated fatty acids, 3-hydroxy fatty acids with a chain length of 16 and 18 carbon atoms and small amounts of 10-methyl-branched fatty acids with 18 carbon atoms (C 19 : 0 10-methyl) affiliated the strains with the Methylobacterium/Xanthobacter group in the Alphaproteobacteria. The G+C content of the DNA is 42?9 mol% (for strain Gr1 T ).The two most dissimilar isolates by 16S rRNA gene comparison (Gr1 T and Gr10; 97?7 % identical)showed 71?5 % DNA-DNA relatedness. Oligonucleotide probes specific for the pure cultures were designed for fluorescence in situ hybridization and demonstrated that two filamentous morphotypes were present in the Grindsted wastewater treatment plant. It is proposed that the isolates represent a new genus and species, Meganema perideroedes gen. nov., sp. nov. The type strain of Meganema perideroedes is strain Gr1 T (=DSM 15528Filamentous bacteria affiliated to the Alphaproteobacteria were isolated from activated sludge biomass from a wastewater treatment plant (WWTP) in Grindsted, Denmark. The treatment plant has carbon removal, the mean cell residence time (sludge age) is 8-10 days, the annual mean temperature is 15-25 u C and the salinity is relatively high, with a conductivity between 15 and 23 mS cm 21 and a sodium concentration around 5-7 g l 21. The plant has been affected by bulking problems for several years, mainly caused by Thiothrix spp. (Nielsen et al., 2000) and other bacteria similar to the isolates reported in this study. Five strains (Gr1 T , Gr2, Gr5, Gr10 and Gr28) were isolated by micromanipulation using a Skerman micromanipulator as described by Blackall (1991) and grown on R2A agar medium (Reasoner & Geldreich, 1985). Gliding motility, Gram-and Neisser-staining (for Gram-reaction and polyphosphate inclusions, respectively; Eikelboom & van Buijsen, 1983) and poly-b-hydroxybutyrate (PHB) inclusions as visualized by Nile blue staining (Ostle & Holt, 1982) were investigated. Only Gr1 T and Gr10 were characterized phenotypically, but they were not able to grow on minimal media (Williams & ...

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Filamentous Alphaproteobacteria Associated with Bulking in Industrial Wastewater Treatment Plants

Cited by 110 publications

References 25 publications

Filamentous bacteria existence in aerobic granular reactors

Filamentous bacteria existence in aerobic granular reactors

Identity, abundance and ecophysiology of filamentous bacteria belonging to the Bacteroidetes present in activated sludge plants

Meganema perideroedes gen. nov., sp. nov., a filamentous alphaproteobacterium from activated sludge

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