Filamentous actin accumulates during plant cell penetration and cell wall plug formation in Phytophthora infestans

Kots, Kiki; Meijer, H.J.G.; Bouwmeester, Klaas; Govers, F.; Ketelaar, Tijs

doi:10.1007/s00018-016-2383-y

Cited by 22 publications

(21 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, a study in Ph. infestans using GFP-labeled F-actin identified an asterlike structure where appressoria contact the leaf, which may focus cargo transport to the penetration peg (Kots et al, 2017). Also, a basic leucine zipper domain transcription factor and mitogen-activated protein kinase were shown to regulate appressorium formation (Blanco and Judelson, 2005;Li et al, 2010).…”

Section: B Box 1 a Diversity Of Infectious Propagulesmentioning

confidence: 99%

Exchanges at the Plant-Oomycete Interface That Influence Disease

Judelson

Ah‐Fong

2018

Plant Physiol.

View full text Add to dashboard Cite

The microbial eukaryotes known as oomycetes comprise more than 1,500 species, including many important phytopathogens. Most exhibit filamentous growth and feed osmotrophically. Oomycetes appear fungus-like but are classified as stramenopiles along with brown algae and diatoms (Beakes et al., 2012). Unlike most fungi, oomycetes are diploid, have cell walls made primarily of cellulose and b-glucans instead of chitin, make aseptate hyphae, undergo oogamous reproduction, and produce few secondary metabolites (Fawke et al., 2015). Oomycetes exhibit diverse lifestyles across terrestrial and aquatic niches. While best known as pathogens of leaves, stems, roots, and fruit, some oomycetes are endophytes, infect animals, or are saprophytes (Lamour and Kamoun, 2009; Ploch and Thines, 2011; Aram and Rizzo, 2018). Many are highly host adapted, unculturable on artificial media, and grow only on living plants as biotrophs. Examples include downy mildew pathogens such as Plasmopara viticola, which infects grapevine (Vitis vinifera), and Albugo candida, which causes white rust on crucifers (Kamoun et al., 2015). The obligate pathogens typically cause minimal damage to the plant but reduce yield and raise susceptibility to secondary infection or abiotic stress. Many oomycetes are hemibiotrophs, which start infections like biotrophs but cause necrosis late in the disease cycle. Most belong to the genus Phytophthora, including Phytophthora cinnamomi, which infects hundreds of agricultural, forest, and ornamental hosts; Phytophthora infestans, which blights potato (Solanum tuberosum) and tomato (Solanum lycopersicum); and Phytophthora sojae, which colonizes soybean (Glycine max) and lupines. Some species, such as Ph. cinnamomi, shift to necrotrophy early in infection, while others, such as Ph. infestans, make the transition much later, reflecting differences in how the species balance the two

show abstract

Section: B Box 1 a Diversity Of Infectious Propagulesmentioning

confidence: 99%

Exchanges at the Plant-Oomycete Interface That Influence Disease

Judelson

Ah‐Fong

2018

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…Actin was a structural component in the plant cell wall and a typical feature in plant defense. 71 The change in actin was mediated by actin depolymerizing factor through anchoring, cross-linking and regulating the network in cell. 72 Actin-depolymerizing factors were negatively correlated with aphid fecundity and population.…”

Section: Relative Fold Expression Of Defense-related Genesmentioning

confidence: 99%

Protein elicitor PeaT1 enhanced resistance against aphid (Sitobion avenae) in wheat

Wang

Yang

et al. 2019

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND Sitobion avenae, a dominant aphid in wheat that causes huge annual losses in agriculture, is mainly controlled using chemical pesticides. In this study, we investigated a protein elicitor, PeaT, for its induction of the defense response in wheat against Sitobion avenae. RESULTS Intrinsic rates of increase in second and third generations of S. avenae decreased in the PeaT1 (second generation 0.28 ± 0.03, third generation 0.26 ± 0.01) treatment compared with controls (second generation 0.31 ± 0.01, third generation 0.28 ± 0.01). S. avenae preferred to colonize control rather than PeaT1‐treated wheat seedlings in a host selection test. PeaT1‐treated wheat leaves possessed more trichomes and wax that formed a disadvantageous surface environment for S. avenae. Both salicylic acid (SA) and jasmonic acid (JA) accumulated significantly in PeaT1‐treated wheat seedlings. CONCLUSION These results showed that PeaT1 modified physical surface structures in wheat to reduce reproduction and deter colonization by S. avenae. SA and JA were involved in the induced physical defense process. This study provided evidence for use of PeaT1 as a ‘vaccine’ to protect wheat from Sitobion avenae. © 2019 Society of Chemical Industry

show abstract

“…After using the platform previously to characterize the protrusive forces exhibited by A. bisexualis hyphae and the relationship between hyphal diameter and force magnitude [20], we used the optimized devices to demonstrate confocal imaging of hyphal tip -pillar interactions. In view of the role the cytoskeleton likely plays in the generation of protrusive force [9], [28]- [31], it is important that the platform is compatible with high-resolution and high-contrast imaging techniques such as confocal microscopy. The images shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of In-Channel High-Aspect Ratio Sensing Pillars for Protrusive Force Measurements on Fungi and Oomycetes

Sun

Tayagui

Garrill

et al. 2018

J. Microelectromech. Syst.

View full text Add to dashboard Cite

This paper reports the fabrication and application of a Lab-on-a-Chip platform containing single-elastomeric micropillars in channel constrictions, which enable the measurement of protrusive forces exerted by individual fungal hyphae. We show the device design, the fabrication process, and photoresist optimization required to adapt the microfluidic platform to relatively thin hyphae. To demonstrate the applicability of the devices, the oomycete Achlya bisexualis and the fungus Neurospora crassa were cultured on PDMS chips. Devices were combined with confocal imaging to study the interaction of A. bisexualis hyphae with the measurement pillars. The force exerted by individual hyphae of N. crassa was measured and compared with a hyphal growth rate and diameter. The platform provides a new tool to help understand the molecular processes that underlie protrusive growth and this may present new ways to tackle the pathogenic growth of these organisms and thus combat the loss of diversity that they cause. This paper is based on the conference proceedings presented at the 31st IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2018), Belfast. [2018-0090] Index Terms-Lab-on-a-chip, force sensor, PDMS micropillars, fungi and oomycetes.

show abstract

Filamentous actin accumulates during plant cell penetration and cell wall plug formation in Phytophthora infestans

Cited by 22 publications

References 47 publications

Exchanges at the Plant-Oomycete Interface That Influence Disease

Exchanges at the Plant-Oomycete Interface That Influence Disease

Protein elicitor PeaT1 enhanced resistance against aphid (Sitobion avenae) in wheat

Fabrication of In-Channel High-Aspect Ratio Sensing Pillars for Protrusive Force Measurements on Fungi and Oomycetes

Contact Info

Product

Resources

About