FijiWings: An Open Source Toolkit for Semiautomated Morphometric Analysis of Insect Wings

Dobens, Alexander C.; Dobens, Leonard L.

doi:10.1534/g3.113.006676

Cited by 33 publications

(40 citation statements)

References 51 publications

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“…Cell density and total trichome number were calculated using the Fijiwings 150px density tool. 45 The total number of trichomes were measured in a 150 £ 150 pixel area located between L4 and L5 veins and immediately distal to the cross vein. Total numbers of trichomes counted ranged from roughly 300-500 within the area measured.…”

Section: Wing Photographymentioning

confidence: 99%

Role ofDrosophilaretinoblastoma protein instability element in cell growth and proliferation

et al. 2015

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The RB tumor suppressor, a regulator of the cell cycle, apoptosis, senescence, and differentiation, is frequently mutated in human cancers. We recently described an evolutionarily conserved C-terminal "instability element" (IE) of the Drosophila Rbf1 retinoblastoma protein that regulates its turnover. Misexpression of wild-type or nonphosphorylatable forms of the Rbf1 protein leads to repression of cell cycle genes. In contrast, overexpression of a defective form of Rbf1 lacking the IE (DIE), a stabilized but transcriptionally less active form of the protein, induced ectopic S phase in cell culture. To determine how mutations in the Rbf1 IE may induce dominant effects in a developmental context, we assessed the impact of in vivo expression of mutant Rbf1 proteins on wing development. DIE expression resulted in overgrowth of larval wing imaginal discs and larger adult wings containing larger cells. In contrast, a point mutation in a conserved lysine of the IE (K774A) generated severely disrupted, reduced wings. These contrasting effects appear to correlate with control of apoptosis; expression of the pro-apoptotic reaper gene and DNA fragmentation measured by acridine orange stain increased in flies expressing the K774A isoform and was suppressed by expression of Rbf1DIE. Intriguingly, cancer associated mutations affecting RB homologs p130 and p107 may similarly induce dominant phenotypes.

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Section: Wing Photographymentioning

confidence: 99%

Role ofDrosophilaretinoblastoma protein instability element in cell growth and proliferation

et al. 2015

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“…Images were captured with cellSens Standard (V1.14) software. Cell density was quantified by counting trichomes on the surface of the wing using the ImageJ FijiWings macro (V2.2) (Dobens and Dobens, 2013). Each trichome represents a single cell (Dobzhansky, 1929).…”

Section: Methodsmentioning

confidence: 99%

Genetic and environmental canalization are not associated among altitudinally varying populations ofDrosophila melanogaster

Pesevski

Dworkin

2019

Preprint

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14Organisms are exposed to environmental and mutational effects influencing both mean and 15 variance of phenotypes. These potentially deleterious effects can be ameliorated by the 16 evolution of buffering (canalizing) mechanisms, resulting in reduced phenotypic variation. 17Under some population genetics models, the circumstances under which genetic canalization 18 evolves is limited. As such, it has been argued that canalizing mechanisms for environmental 19 and mutational stresses may co-evolve, and will be correlated. Yet, empirical evidence has 20 not consistently supported this prediction. In a recent study, a population of Drosophila 21 melanogaster adapted to high altitude showed evidence of genetic decanalization relative to 22 low-altitude populations. Using these populations, we tested if these populations also var-23 ied for environmental canalization. We reared strains derived from these populations under 24 different temperature rearing environments. Using the Drosophila wing, we quantified size, 25 shape, cell density and frequencies of mutational defects. We observed the expected differ-26 ences in size, shape, cell density and mutational defects between the high-and low-altitude 27 populations. However, we observed little evidence for a relationship between a number of 28 measures of environmental canalization with population of origin or visible defect frequency. 29Our results do not support the predicted association between genetic and environmental 30 canalization. 31

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“…1D,E ;Yoffe et al, 1995). Misexpression of Trbl in this domain has two effects on wing morphology (noted in Dobens and Dobens, 2013;Mata et al, 2000):…”

Section: The Trbl Wing Misexpression Phenotype Shows Interactions Witmentioning

confidence: 99%

A screen for targets of the Drosophila pseudokinase Tribbles identifies Neuralized and Mindbomb, ubiquitin ligases that mediate Notch signaling

Shipman

Nauman

Haymans

et al. 2018

Preprint

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and stabilizing Mindbomb and indicate that a wing misexpression approach is useful to identify novel components in a conserved Tribbles signaling pathway. AUTHOR SUMMARY (99 words)Tribbles pseudokinases are adaptor molecules, binding diverse targets regulating cell differentiation, growth and proliferation and directing their proteasomal degradation. To search for novel targets of Drosophila Tribbles, we adopted a wing co-misexpression scheme and measured changes in cell/tissue size to identify enhancers and suppressors of the Tribbles phenotype. We show the Notch pathway components Neuralized and Mindbomb1 E3 ligases act as Tribbles suppressors and demonstrate that Tribbles modulates their levels and activites. Recent demonstration that mammalian Tribbles 3 binds the E3 ligase Mindbomb to promote ligand-mediated Notch activation implies a conserved role for Tribbles family members in Notch activation.

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FijiWings: An Open Source Toolkit for Semiautomated Morphometric Analysis of Insect Wings

Cited by 33 publications

References 51 publications

Role ofDrosophilaretinoblastoma protein instability element in cell growth and proliferation

Role ofDrosophilaretinoblastoma protein instability element in cell growth and proliferation

Genetic and environmental canalization are not associated among altitudinally varying populations ofDrosophila melanogaster

A screen for targets of the Drosophila pseudokinase Tribbles identifies Neuralized and Mindbomb, ubiquitin ligases that mediate Notch signaling

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