Fifteen new nucleotide substitutions in variants of human papillomavirus 18 in Korea

Kim, Namhee; Park, Jeong Su; Kim, Ji Eun; Park, Jae Hyeon; Park, Hyunwoong; Roh, Eun Youn; Yoon, Jong Hyun; Shin, Sue

doi:10.1186/s12985-020-01337-7

Cited by 5 publications

(3 citation statements)

References 33 publications

(53 reference statements)

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“…In our population, eight substitutions were found in HPV18 variants which were not previously reported in the country. Following further examination, we found these mutations previously reported by Korean researchers [ 18 ], a finding that supports the geographical distribution of HPV lineages. Previous studies have found that HPV18 lineage B could be associated with a higher risk of CC than lineage A, and a higher risk of persistence and progression [ 60 , 66 ].…”

Section: Discussionsupporting

confidence: 88%

“…Furthermore, HPV16 sublineages A3, A4, and D have been reported to be associated with higher risks of CC [ 14 – 16 ]. Conversely, most studies do not support any of HPV18 lineages or sublineages carrying a higher risk of cancer compared to others [ 17 , 18 ]. However, these findings were not replicated globally and the inconsistent results highlight the necessity of further studies on the distribution of HPV16/18 lineages and sublineages in different regions and their oncogenicity considering ethnicity.…”

Section: Introductionmentioning

confidence: 97%

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Type distribution of human papillomaviruses in ThinPrep cytology samples and HPV16/18 E6 gene variations in FFPE cervical cancer specimens in Fars province, Iran

Farhadi,

Abuei,

Okhovat

et al. 2023

Cancer Cell Int

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Background There exists strong evidence that human papillomavirus (HPV) is associated with cervical cancer (CC). HPV E6 is a major oncogene whose sequence variations may be associated with the development of CC. There is not sufficient data on the distribution of HPV types in ThinPrep cytology specimens and HPV 16/18 E6 gene variations among CC patients in the southwest of Iran. This study was conducted to contribute to HPV screening and vaccination in Iran. Methods A total of 648 women screened for cervicitis, intraepithelial neoplasia or CC were included in the study. All participants underwent ThinPrep cytology testing, single-step HPV DNA detection and allele-specific reverse hybridization assays. Moreover, a total of 96 specimens previously tested positive for single infection with HPV16 or 18 were included for variant analysis. HPV16/18 lineages and sublineages were determined by PCR assays followed by sequencing the E6 gene and the construction of neighbor-joining phylogenetic trees. Results Overall, HPV DNA was detected in 62.19% of all the screened subjects. The detection rates of HPV DNA among individuals with normal, ASC-US, ASC-H, LSIL, and HSIL cervical cytology were 48.9%, 93.6%, 100%, 100%, and 100%, respectively. Low-risk HPVs were detected more frequently (46.9%) than high-risk (38.9%) and possible high-risk types (11.1%). Of 403 HPV-positive subjects, 172 (42.7%) had single HPV infections while the remaining 231 (57.3%) were infected with multiple types of HPV. Our results indicated a remarkable growth of high-risk HPV66 and 68 and low-risk HPV81 which have rarely been reported in Iran and HPV90 and 87 that are reported for the first time in the country. In addition, 3 lineages (A, D, and C) and 6 sublineages (A1, A2, A4, C1, D1, and D2) of HPV16, and one lineage and 4 sublineages (A1, A3, A4, and A5) of HPV18 were identified. The studied HPV16 and 18 variants mainly belonged to the D1 and A4 sublineages, respectively. Conclusion The present study suggests that the prevalence of HPV infection in women of all age groups with or without premalignant lesions in the southwestern Iran is high and the predominant HPV types in the southwest of Iran may differ from those detected in other parts of the country. This study also highlights the necessity of not only initiating HPV vaccination for the general population but also developing new vaccines that confer immunity against the prevalent HPV types in the area and national cervical screening programs using a combination of thinPrep cytology test and HPV detection assays in order to improve the accuracy of the screening.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 97%

Type distribution of human papillomaviruses in ThinPrep cytology samples and HPV16/18 E6 gene variations in FFPE cervical cancer specimens in Fars province, Iran

Farhadi,

Abuei,

Okhovat

et al. 2023

Cancer Cell Int

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“…Moreover, sublineage A1 comprised 91.7%. Fifteen new nucleotide substitutions were identified among 44 nucleotide substitutions from which six substitutions at positions 317, 443, 5467, 5560, 6462, and 6823 resulted in amino acid changes in E6 including F71L, N113K; L1: H13R, H44P, A345T, and N465S [ 32 ]. Among the reference sublineages, A3 was reported to harbor amino acid changes from E to G at position 43 in contrast to other HPV18 sublineages.…”

Section: Discussionmentioning

confidence: 99%

Determination of Human Papillomavirus Type 18 Lineage of E6: A Population Study from Iran

Larijani

Omrani

Soleimani‐Jelodar

et al. 2022

BioMed Research International

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The epidemiological studies in Iran on HPV18 nucleotide changes are rare. This type of virus is prevalent in the Iranian population. Therefore, in the present study, we aimed to identify the genetic variability in HPV18 in the E6 region to evaluate the prevalence of lineage distribution and sublineages in a sample population in Iran. Overall, 60 HPV18 confirmed cases were investigated between 2019 and 2021. The specimens were collected, and molecular genotyping was done using the Linear Array HPV Genotyping Test. DNA extraction was performed by a viral DNA/RNA kit. The HPV E6 gene was amplified by using type-specific primers designed according to the HPV18 genome prototype sequence. The sequencing of the E6 region was successfully done on 43 samples which were then compared to the reference sequence. The most frequent sublineage of HPV18 in this study was A4 (69.7%), followed by A1 (18.6%) and A3 (11.6%). Neither A2 nor A5 sublineage was not detected in this study. The related nucleotide acid changes according to the main references were as follows: A3: T104C/T232G/T485C/C549A, A4: T104C/T485C/C549A. The predominance of A lineage with the high frequency of A4 sublineage was found in the present research. The importance of sublineages in susceptibility to a progressive form of infection requires to be more investigated among the different population.

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HPV prevalence and genotype distribution among Vietnamese cervical cancer patients in the northern region of Vietnam, from 2018 – 2020

Van Nam,

Son,

Bang

et al. 2024

Journal of Clinical Virology Plus

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Fifteen new nucleotide substitutions in variants of human papillomavirus 18 in Korea

Cited by 5 publications

References 33 publications

Type distribution of human papillomaviruses in ThinPrep cytology samples and HPV16/18 E6 gene variations in FFPE cervical cancer specimens in Fars province, Iran

Type distribution of human papillomaviruses in ThinPrep cytology samples and HPV16/18 E6 gene variations in FFPE cervical cancer specimens in Fars province, Iran

Determination of Human Papillomavirus Type 18 Lineage of E6: A Population Study from Iran

HPV prevalence and genotype distribution among Vietnamese cervical cancer patients in the northern region of Vietnam, from 2018 – 2020

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