Fifteen microsatellite markers for the stripe-necked terrapin Mauremys caspica (Testudines: Geoemydidae) and cross-amplification tests in M. rivulata

Vamberger, Melita; Stuckas, Heiko; Fritz, Uwe

doi:10.1007/s12686-010-9297-z

Cited by 13 publications

(13 citation statements)

References 5 publications

(4 reference statements)

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“…For each sample, 14 unlinked microsatellite loci as characterized by Vamberger et al (2011) were studied. Four or five loci each were combined in multiplex PCRs.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

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Conservation genetics and phylogeography of the poorly known Middle Eastern terrapin Mauremys caspica (Testudines: Geoemydidae)

et al. 2012

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The West Asian stripe-necked terrapin Mauremys caspica is widespread throughout the Middle East-a region for which only few phylogeographic studies are available. Due to landscape alteration, pollution and intensification of water management, M. caspica is increasingly threatened. However, genetic diversity among and within populations is poorly known, impeding the identification of management units. Using a nearly rangewide sampling, we analyzed 14 microsatellite loci and mtDNA sequences in order to gain insight into the population structure and history of M. caspica.In agreement with a previous study, we found two clusters of mitochondrial haplotypes, with one cluster distributed in the east and the other in the west of the range. However, our microsatellite data suggested a more pronounced geographical structuring. When null alleles were coded as recessive with STRUCTURE 2.3.2, three clusters were revealed, with one cluster matching roughly the range of the western mitochondrial cluster, and the composite ranges of the two other microsatellite clusters correspond to the distribution of the eastern mitochondrial cluster. Naïve STRUCTURE analyses without correction for null alleles were congruent with respect to the two eastern microsatellite clusters, but subdivided the western cluster into two units, with an additional geographical divide corresponding to the 'Anatolian diagonal'-a wellknown high mountain barrier impeding exchange between western and eastern taxa. In naïve analyses, the westernmost microsatellite cluster (from Central Anatolia) is quite isolated from the others, and its distinctness is also supported by fixation indices resembling the values among the other three clusters. One of the two eastern clusters is distributed in the Caucasus region plus Iran, and terrapins from Saudi Arabia and Bahrain constitute the second eastern cluster, supporting Electronic supplementary material The online version of this article (

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“…For each sample, 14 unlinked microsatellite loci as characterized by Vamberger et al (2011) were studied. Four or five loci each were combined in multiplex PCRs.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

“…Besides the mitochondrial cyt b gene, we use 14 rapidly evolving microsatellite loci (Vamberger et al 2011) to achieve a better understanding of the phylogeography and population structuring of M. caspica. In particular, we re-assess the status of the critically endangered Arabian populations.…”

Section: Introductionmentioning

confidence: 99%

Conservation genetics and phylogeography of the poorly known Middle Eastern terrapin Mauremys caspica (Testudines: Geoemydidae)

et al. 2012

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“…Three hundred and twenty‐eight samples were genotyped for 13 unlinked microsatellite loci (Vamberger et al . ). Three to five loci each were combined in multiplex PCRs (Table S2).…”

Section: Methodsmentioning

confidence: 97%

“…Therefore, we re‐examine in the present paper geographically correlated genetic variation within M. rivulata based on a rangewide sampling and length polymorphisms of 13 unlinked microsatellite loci (Vamberger et al . ) and compare this information with the mitochondrial differentiation pattern using the cyt b gene. For doing so, we expand the sampling of the two previous studies (Mantziou et al .…”

Section: Introductionmentioning

confidence: 99%

Massive transoceanic gene flow in a freshwater turtle (Testudines: Geoemydidae: Mauremys rivulata)

et al. 2014

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The freshwater turtle Mauremys rivulata ranges from the Adriatic coast of the Balkan Peninsula through the Aegean region and coastal western and southern Turkey southwards to Israel. In addition, it occurs on several Aegean islands, Crete and Cyprus. Previous investigations using mtDNA sequences found virtually no genetic differentiation across its distribution range, despite some major biogeographical barriers for terrestrial and freshwater biota. Thus, the absence of any phylogeographical differentiation would be unexpected. To re‐examine genetic differentiation within M. rivulata, here we use a comprehensive rangewide sampling and information of 13 unlinked polymorphic microsatellite loci and compare these data against mtDNA variation. Our microsatellite analyses reveal a weak population structuring which conflicts, however, with most biogeographical barriers. We conclude that the genetic structure in the vast majority of the species' range has been shaped by massive transoceanic gene flow. This explanation is unlikely for the northernmost populations, which seem rather to be genetically impacted by intentionally released foreign turtles.

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“…Each sample was genotyped at 13 unlinked microsatellite loci as characterized by Vamberger et al . (), and the cytochrome b gene (cyt b ) was sequenced as a mitochondrial marker. As microsatellites are known to detect only recent hybrid and backcross generations (Sanz et al .…”

Section: Methodsmentioning

confidence: 99%

Unexpected hybridization patterns in Near Eastern terrapins (Mauremys caspica, M. rivulata) indicate ancient gene flow across the Fertile Crescent

et al. 2016

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U. (2017). Unexpected hybridization patterns in Near Eastern terrapins (Mauremys caspica, M. rivulata) indicate ancient gene flow across the Fertile Crescent. -Zoologica Scripta, 46, 401-413.Recent studies indicate that hybridization in animals occurs more frequently than previously thought and that it may play an important evolutionary role. Chelonians are capable of extensive hybridization, raising the question how chelonian species evolve and maintain genetic integrity despite hybridization. Here, we use two sister species with parapatric distribution, Mauremys caspica and M. rivulata, as our model. These taxa are estimated to have diverged some 5.3-7.0 million years ago. Using rangewide sampling and 13 unlinked polymorphic microsatellite markers, five nuclear loci and one mitochondrial gene, we show that hybridization is rare along the contact zone of the two species in Turkey. However, we discovered an unexpected hybrid swarm in the southern Levant that has been hitherto identified with M. rivulata. This hybrid swarm is separated from the inland species M. caspica by a 700-km-wide distribution gap corresponding to the Syrian Desert. Ecological palaeomodelling suggests that during more humid climatic episodes in the Last Glacial Maximum and mid-Holocene, the current contact zone extended into the southern Levant, facilitating the establishment of the now isolated hybrid swarm. Our results support that there is not necessarily a general hybridization pattern in a given species couple and that the extent of gene flow may differ considerably in different parts of the distribution range. Moreover, our results highlight that studies on hybridization should not focus only on extant contact and hybrid zones, but should use rangewide sampling to detect signals of ancient hybridization that might otherwise be missed.

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Fifteen microsatellite markers for the stripe-necked terrapin Mauremys caspica (Testudines: Geoemydidae) and cross-amplification tests in M. rivulata

Cited by 13 publications

References 5 publications

Conservation genetics and phylogeography of the poorly known Middle Eastern terrapin Mauremys caspica (Testudines: Geoemydidae)

Conservation genetics and phylogeography of the poorly known Middle Eastern terrapin Mauremys caspica (Testudines: Geoemydidae)

Massive transoceanic gene flow in a freshwater turtle (Testudines: Geoemydidae: Mauremys rivulata)

Unexpected hybridization patterns in Near Eastern terrapins (Mauremys caspica, M. rivulata) indicate ancient gene flow across the Fertile Crescent

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