Field validation of a Leishmania (Leishmania) mexicana exo-antigens ELISA for diagnosing tegumentary leishmaniasis in regions of Leishmania (Viannia) predominance

Soares, Killarney Ataide; Urdapilleta, Ada Amalia Ayala; Santos, Gilcilene Maria dos; Carneiro, Andrea Lisboa; Gomes, Ciro Martins; Roselino, Ana Maria Ferreira; Sampaio, Raimunda Nonata Ribeiro

doi:10.1016/j.bjid.2015.03.010

Cited by 8 publications

(4 citation statements)

References 29 publications

(32 reference statements)

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“…Currently available parasitological tests based on stained smears and culture offer low sensitivity, and other options, such as the Montenegro (leishmanin) skin test (MST), have high sensitivity but low specificity (3,6,7). Additionally, serological techniques may yield widely varied accuracy values (3).…”

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confidence: 99%

Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic

et al. 2017

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The precise diagnosis of American tegumentary leishmaniasis (ATL) is an essential task due to the disease's associated morbidity. A noninvasive, extremely sensitive, and highly specific exam is critical, particularly for mucosal leishmaniasis (ML), in which a low parasite quantity is expected. We aimed to compare the diagnostic accuracy of swab and biopsy sample analysis using SYBR Green-and TaqManbased real-time PCR (qPCR) assays with that of a composite reference standard consisting of the Montenegro skin test, serology, histopathology, smears, culture, and conventional PCR. In total, 55 patients with ATL (ML, 18 patients; cutaneous leishmaniasis [CL], 37 patients) and 36 patients without ATL were studied. qPCR analysis of swabs was more accurate when using SYBR Green (87.88%; 95% confidence interval [CI], 77.86 to 93.73 patients) than when using TaqMan (78.79%; 95% CI, 67.49 to 86.92%) (P ϭ 0.031). SYBR Green (84.72%; 95% CI, 74.68 to 91.25%) was also more accurate than TaqMan (73.61%; 95% CI, 62.42 to 82.41%) for biopsy samples (P ϭ 0.008). All qPCR methods were 100% specific. Swabs and biopsy specimens had similar sensitivity when using the same chemistry (P ϭ 0.125 for SYBR Green and P ϭ 0.625 for TaqMan). Moreover, qPCR achieved better performance than most existing techniques used for the diagnosis of ATL and also detected the Leishmania parasite in a greater proportion of patients than the associated histopathology, smear, culture, and conventional PCR techniques did. Swabs therefore represent a useful diagnostic tool because they not only are noninvasive but also can achieve an accuracy similar to that of biopsy samples. The high accuracy of SYBR Green-based qPCR may also reduce the requirement for associated parasitological tests for ATL diagnosis.

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confidence: 99%

Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic

et al. 2017

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“…Also, non-infective promastigotes can be lysed through the activity of neutrophil extracellular traps although metacyclic promastigotes are resistant to this innate immunity mechanism (Guimaraes-Costa et al, 2009 , 2014 ; Hurrell et al, 2016 ). The release of different intracellular antigenic proteins contained in secreted vesicles (Silverman et al, 2008 ; Cuervo et al, 2009 ; Torrecilhas et al, 2012 ) may be also an alternative form of presentation of those antigens to the host immune system, since Leishmania is able to release microvesicles in the mammalian host (Silverman and Reiner, 2012 ) and their contents have been found to induce humoral responses in murine susceptible hosts (Hernández-Chinea, 2007 ), canine (Lima et al, 2016 ), and human patients (Soares et al, 2015 ) as well as inhibitory signaling for dendritic cells activation (Markikou-Ouni et al, 2015 ; Iborra et al, 2016 ; von Stebut and Tenzer, 2018 ). Interestingly, whereas in patients with the active form of the disease they induce strong humoral responses (Requena et al, 2000a ; Maalej et al, 2003 ; Rafati et al, 2007 ; Costa et al, 2012 ; Souza et al, 2013 ) as well as IL-10 mediated responses (Bottrel et al, 2001 ; de Carvalho et al, 2003 ; Antonelli et al, 2004 ; Carvalho et al, 2005 ) in asymptomatic or in cured patients these proteins usually induce Th1-like responses (Probst et al, 2001 ; Bourreau et al, 2003 ; Baharia et al, 2014 ; Jaiswal et al, 2014 ; Cecilio et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis

Garde

Ramírez

Corvo

et al. 2018

Front. Cell. Infect. Microbiol.

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Different members of intracellular protein families are recognized by the immune system of the vertebrate host infected by parasites of the genus Leishmania. Here, we have analyzed the antigenic and immunogenic properties of the Leishmania eIF2 and eIF2B translation initiation factors. An in silico search in Leishmania infantum sequence databases allowed the identification of the genes encoding the α, β, and γ subunits and the α, β, and δ subunits of the putative Leishmania orthologs of the eukaryotic initiation factors F2 (LieIF2) or F2B (LieIF2B), respectively. The antigenicity of these factors was analyzed by ELISA using recombinant versions of the different subunits. Antibodies against the different LieIF2 and LieIF2B subunits were found in the sera from human and canine visceral leishmaniasis patients, and also in the sera from hamsters experimentally infected with L. infantum. In L. infantum (BALB/c) and Leishmania major (BALB/c or C57BL/6) challenged mice, a moderate humoral response against these protein factors was detected. Remarkably, these proteins elicited an IL-10 production by splenocytes derived from infected mice independently of the Leishmania species employed for experimental challenge. When DNA vaccines based on the expression of the LieIF2 or LieIF2B subunit encoding genes were administered in mice, an antigen-specific secretion of IFN-γ and IL-10 cytokines was observed. Furthermore, a partial protection against murine CL development due to L. major infection was generated in the vaccinated mice. Also, in this work we show that the LieIF2α subunit and the LieIF2Bβ and δ subunits have the capacity to stimulate IL-10 secretion by spleen cells from naïve mice. B-lymphocytes were identified as the major producers of this anti-inflammatory cytokine. Taking into account the data found in this study, it may be hypothesized that these proteins act as virulence factors implicated in the induction of humoral responses as well as in the production of the down-regulatory IL-10 cytokine, favoring a pathological outcome. Therefore, these proteins might be considered markers of disease.

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“…and anti- T. cruzi antibodies has been overlooked. In contrast, exoantigens of T. cruzi 15 , and Leishmania spp 16 have been studied, have performed well in the diagnosis of CD 17 , 18 , and have also been promising for the diagnosis of VL 19 - 21 and ATL 20 - 23 . …”

Section: Short Communicationmentioning

confidence: 99%

Leptomonas seymouri and Crithidia fasciculata exoantigens can discriminate human cases of visceral leishmaniasis from American tegumentary leishmaniasis ones

Kesper

Teixeira

Lindoso

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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Field validation of a Leishmania (Leishmania) mexicana exo-antigens ELISA for diagnosing tegumentary leishmaniasis in regions of Leishmania (Viannia) predominance

Cited by 8 publications

References 29 publications

Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic

Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic

Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis

Leptomonas seymouri and Crithidia fasciculata exoantigens can discriminate human cases of visceral leishmaniasis from American tegumentary leishmaniasis ones

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